Entry |
|
Name |
hyaluronan synthase;
spHAS;
seHAS;
Alternating UDP-alpha-N-acetyl-D-glucosamine:beta-D-glucuronosyl-(1->3)-[nascent hyaluronan] 4-N-acetyl-beta-D-glucosaminyltransferase and UDP-alpha-D-glucuronate:N-acetyl-beta-D-glucosaminyl-(1->4)-[nascent hyaluronan] 3-beta-D-glucuronosyltransferase
|
Class |
Transferases;
Glycosyltransferases;
Hexosyltransferases
|
Sysname |
Alternating UDP-N-acetyl-alpha-D-glucosamine:beta-D-glucuronosyl-(1->3)-[nascent hyaluronan] 4-N-acetyl-beta-D-glucosaminyltransferase and UDP-alpha-D-glucuronate:N-acetyl-beta-D-glucosaminyl-(1->4)-[nascent hyaluronan] 3-beta-D-glucuronosyltransferase (configuration-inverting)
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Reaction(IUBMB) |
(1) UDP-N-acetyl-alpha-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-[nascent hyaluronan] = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-[nascent hyaluronan] [RN: R05327 R06068];
(2) UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-[nascent hyaluronan] = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-[nascent hyaluronan]
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Reaction(KEGG) |
|
Substrate |
UDP-N-acetyl-alpha-D-glucosamine [CPD: C00043];
beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-[nascent hyaluronan];
UDP-alpha-D-glucuronate [CPD: C00167];
N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-[nascent hyaluronan]
|
Product |
UDP [CPD: C00015];
N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-[nascent hyaluronan];
beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-[nascent hyaluronan]
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Comment |
The enzyme from Streptococcus Group A and Group C requires Mg2+. The enzyme adds GlcNAc to nascent hyaluronan when the non-reducing end is GlcA, but it adds GlcA when the non-reducing end is GlcNAc [3]. The enzyme is highly specific for UDP-GlcNAc and UDP-GlcA; no copolymerization is observed if either is replaced by UDP-Glc, UDP-Gal, UDP-GalNAc or UDP-GalA. Similar enzymes have been found in a variety of organisms.
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History |
EC 2.4.1.212 created 2001, modified 2007
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Orthology |
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Genes |
» show all
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Reference |
|
Authors |
DeAngelis PL, Papaconstantinou J, Weigel PH |
Title |
Molecular cloning, identification, and sequence of the hyaluronan synthase gene from group A Streptococcus pyogenes. |
Journal |
J Biol Chem 268:19181-4 (1993) |
Sequence |
|
Reference |
|
Authors |
Jing W, DeAngelis PL. |
Title |
Dissection of the two transferase activities of the Pasteurella multocida hyaluronan synthase: two active sites exist in one polypeptide. |
Journal |
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Sequence |
|
Reference |
|
Authors |
DeAngelis PL. |
Title |
Molecular directionality of polysaccharide polymerization by the Pasteurella multocida hyaluronan synthase. |
Journal |
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Reference |
|
Authors |
Tlapak-Simmons VL, Baron CA, Weigel PH. |
Title |
Characterization of the purified hyaluronan synthase from Streptococcus equisimilis. |
Journal |
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Other DBs |
ExPASy - ENZYME nomenclature database: | 2.4.1.212 |
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LinkDB |
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