KEGG   ENZYME: 2.4.1.232
Entry
EC 2.4.1.232                Enzyme                                 
Name
initiation-specific alpha-1,6-mannosyltransferase;
alpha-1,6-mannosyltransferase;
GDP-mannose:oligosaccharide 1,6-alpha-D-mannosyltransferase;
GDP-mannose:glycolipid 1,6-alpha-D-mannosyltransferase;
glycolipid 6-alpha-mannosyltransferase;
GDP-mannose:oligosaccharide 1,6-alpha-D-mannosyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
GDP-mannose:oligosaccharide 6-alpha-D-mannosyltransferase
Reaction(IUBMB)
Transfers an alpha-D-mannosyl residue from GDP-mannose into lipid-linked oligosaccharide, forming an alpha-(1->6)-D-mannosyl-D-mannose linkage
Reaction(KEGG)
(other) R06986(G) R08599(G)
Comment
Requires Mn2+. In Saccharomyces cerevisiae, this enzyme catalyses an essential step in the outer chain elongation of N-linked oligosaccharides. Man8GlcNAc and Man9GlcNAc are equally good substrates.
History
EC 2.4.1.232 created 2004
Pathway
ec00513  Various types of N-glycan biosynthesis
ec01100  Metabolic pathways
Orthology
K05528  alpha 1,6-mannosyltransferase
K05529  mannan polymerase complexes MNN9 subunit
Genes
MNG: MNEG_9768
CME: CYME_CMT636C
GSL: Gasu_17110 Gasu_59940
SCE: YGL038C(OCH1) YPL050C(MNN9)
SEUB: DI49_1929 DI49_5408
SPAO: SPAR_G01980 SPAR_P02160
AGO: AGOS_AFR465C AGOS_AGL259C
ERC: Ecym_1409 Ecym_2550
KLA: KLLA0_C04125g KLLA0_E12849g KLLA0_F22220g
KMX: KLMA_10184(och1) KLMA_40267(OCH1) KLMA_70378(MNN9)
LTH: KLTH0E11616g KLTH0H09064g
VPO: Kpol_1026p11 Kpol_1031p78
ZRO: ZYRO0F08492g ZYRO0G09284g
CGR: 2886464(GVI51_A01485) 2890611(GVI51_L12749)
NCS: NCAS_0A01880(NCAS0A01880) NCAS_0B02440(NCAS0B02440) NCAS_0C02330(NCAS0C02330)
NDI: NDAI_0C05250(NDAI0C05250) NDAI_0D03810(NDAI0D03810) NDAI_0E03020(NDAI0E03020)
TPF: TPHA_0F00500(TPHA0F00500) TPHA_0K01810(TPHA0K01810) TPHA_0L01370(TPHA0L01370)
TBL: TBLA_0B00380(TBLA0B00380) TBLA_0C04290(TBLA0C04290) TBLA_0H03340(TBLA0H03340)
TGB: HG536_0D04830 HG536_0H01410
TDL: TDEL_0B01370(TDEL0B01370) TDEL_0F02760(TDEL0F02760)
KAF: KAFR_0F03660(KAFR0F03660) KAFR_0H01390(KAFR0H01390)
KNG: KNAG_0A02150(KNAG0A02150) KNAG_0A03990(KNAG0A03990) KNAG_0D03770(KNAG0D03770)
ZMK: HG535_0B05480 HG535_0C01350
SLUD: SCDLUD_001544 SCDLUD_001970
DHA: DEHA2C13508g DEHA2D15972g
PIC: PICST_56149 PICST_76016(OCH1)
PGU: PGUG_00592 PGUG_05622
SPAA: SPAPADRAFT_143242 SPAPADRAFT_143660 SPAPADRAFT_143760 SPAPADRAFT_155280(OCH1) SPAPADRAFT_49565 SPAPADRAFT_52588 SPAPADRAFT_53501 SPAPADRAFT_59962 SPAPADRAFT_61486 SPAPADRAFT_62310 SPAPADRAFT_62316 SPAPADRAFT_62531 SPAPADRAFT_72461
LEL: PVL30_003578(OCH1) PVL30_003659(MNN9)
LBG: 92208561(LODBEIA_P33650) 92208637(LODBEIA_P34410)
CAL: CAALFM_C306020WA(MNN9) CAALFM_C306090CA(OCH1)
CTP: CTRG_02254 CTRG_02261
COT: CORT_0C02360 CORT_0C04350
CDU: CD36_85950(MNN9) CD36_86020
CTEN: 18250825(OCH1)
CLU: CLUG_01658 CLUG_02025
CLUS: A9F13_02g02673 A9F13_13g01012
CAUR: CJI96_0000373 CJI96_0002796
PPA: PAS_chr1-3_0251 PAS_chr4_0103
PKZ: C5L36_0A08300 C5L36_0B02277 C5L36_0B07470 C5L36_0B08630 C5L36_0C02090
BNN: FOA43_003835(MNN9) FOA43_004150
BBRX: BRETT_000545 BRETT_002798 BRETT_003985
OPA: HPODL_01382 HPODL_01489 HPODL_01625 HPODL_02407 HPODL_02408 HPODL_03209 HPODL_04510 HPODL_04840
YLI: 2907625(YALI2_C00375g)
SLB: AWJ20_134(HOC1) AWJ20_191(MNN9)
NCR: NCU00609(och-1) NCU02635
NTE: NEUTE1DRAFT126859(NEUTE1DRAFT_126859) NEUTE1DRAFT91925(NEUTE1DRAFT_91925)
SMP: 10803572(SMAC4_05727) 10810094(SMAC4_01297)
PAN: PODANSg3939 PODANSg5619
PBEL: QC761_109230(MNN9) QC761_114710(och1)
PPSD: QC762_109230(MNN9) QC762_114710(och1)
PPSP: QC763_109230(MNN9) QC763_114710(och1)
PPSA: QC764_109230(MNN9) QC764_114710(och1)
TTT: THITE_2095073 THITE_2121506
MTM: MYCTH_2106248 MYCTH_2297411 MYCTH_60177
CTHR: CTHT_0014860 CTHT_0019180 CTHT_0057910
MGR: MGG_00171 MGG_02859 MGG_05011 MGG_08652
PPEI: PpBr36_03389 PpBr36_05778 PpBr36_08977 PpBr36_09260 PpBr36_10347 PpBr36_10775
PGRI: PgNI_01294 PgNI_08023 PgNI_10515 PgNI_11737
TMN: UCRPA7_2404 UCRPA7_8569
SSCK: SPSK_09242 SPSK_09403
FGR: FGSG_06856 FGSG_09425
FPU: FPSE_07960 FPSE_11809
FPOA: FPOAC1_010599 FPOAC1_012243
FVN: FVRRES_11598 FVRRES_13327
FVR: FVEG_03975 FVEG_05131
FOX: FOXG_01971 FOXG_06111
NHE: NECHADRAFT_105919 NECHADRAFT_123326
FFC: NCS54_00286700 NCS54_00561600
FKR: NCS57_00282000 NCS57_00599500
FMU: J7337_002618 J7337_003980
TRE: TRIREDRAFT_58609 TRIREDRAFT_65646 TRIREDRAFT_80340
TRR: M419DRAFT_124397 M419DRAFT_131661 M419DRAFT_74592
TATV: 25775360(TrAtP1_010807) 25784343(TrAtP1_012924) 90959456(TrAtP1_004198)
TASP: 36608430(TrAFT101_009823) 36611486(TrAFT101_003671) 36617403(TrAFT101_009279)
MAW: 19245306(MNN9) 19252792(och1_2)
MAJ: MAA_05737 MAA_08458
MBRN: 26238696(MNN9) 26244212(OCH1_0)
PCHM: VFPPC_00774 VFPPC_02981 VFPPC_10118
CMT: CCM_05217 CCM_06374
AMUS: LMH87_004972 LMH87_011232
PLJ: 28887492(PLICBS_002740) 28888152(PLICBS_003360)
PTKZ: JDV02_000482 JDV02_001108(MNN9)
VAL: VDBG_02349 VDBG_04673
VDA: VDAG_02820 VDAG_04214
CFJ: CFIO01_01535 CFIO01_03025
CLUP: CLUP02_05583
CHIG: CH63R_00695 CH63R_07086 CH63R_09786
CDET: 87939413(CDEST_02910) 87944260(CDEST_07757) 87950865(CDEST_14365)
SAPO: SAPIO_CDS10094 SAPIO_CDS3303 SAPIO_CDS7989
ELA: UCREL1_1692 UCREL1_7932 UCREL1_801 UCREL1_886
PFY: PFICI_00433 PFICI_09035 PFICI_09794 PFICI_14506
SSL: SS1G_02807 SS1G_03158
BFU: BCIN_06g03220(Bcmnn9) BCIN_08g03990
MBE: MBM_04797 MBM_07558
PSCO: LY89DRAFT_313255 LY89DRAFT_594840 LY89DRAFT_676037 LY89DRAFT_699034
GLZ: GLAREA_01840 GLAREA_05012 GLAREA_05067 GLAREA_10699
ANI: ANIA_04716 ANIA_07672
AFM: AFUA_2G01450 AFUA_5G08580
ACT: ACLA_011950 ACLA_033030 ACLA_093460
NFI: NFIA_033750 NFIA_078700
AOR: AO090120000208 AO090701000814
ANG: An03g05010 An07g04940
AFV: AFLA_005322 AFLA_009426
ALUC: AKAW2_11724A(OCH1_1) AKAW2_50871A(MNN9)
ACHE: ACHE_40559A(MNN9) ACHE_50551A(OCH1)
APUU: APUU_71012S(OCH1) APUU_80223A(MNN9)
PCS: N7525_004734 N7525_008975
PDP: PDIP_07370 PDIP_51700 PDIP_63540
POU: POX_a00184 POX_b03284
TMF: EYB26_000622 EYB26_004026
TRG: TRUGW13939_00569 TRUGW13939_03932 TRUGW13939_10718
CIM: CIMG_00141 CIMG_06774
CPW: 9692242(MNN9) 9695506(OCH1)
URE: UREG_00247 UREG_03690
ABE: ARB_05752 ARB_06643
TVE: TRV_02425 TRV_04489
PBL: PAAG_03802 PAAG_04051 PAAG_05983
PBN: PADG_03306 PADG_06012 PADG_07327
AJE: HCAG_03327
BGH: BDBG_01145 BDBG_06843
PNO: SNOG_02920 SNOG_10597
PTE: PTT_09202 PTT_11542 PTT_19588
PTRR: 6339861(PtrM4_027550) 6348321(PtrM4_083700)
BZE: COCCADRAFT_10069 COCCADRAFT_26402 COCCADRAFT_41957 COCCADRAFT_6319 COCCADRAFT_95570
BSC: COCSADRAFT_173170 COCSADRAFT_232790 COCSADRAFT_40014 COCSADRAFT_41481
BOR: COCMIDRAFT_101902 COCMIDRAFT_34243 COCMIDRAFT_368 COCMIDRAFT_4455
AALT: CC77DRAFT_1030960 CC77DRAFT_1052656 CC77DRAFT_361229
ADAC: 96082643(ACET3X_002321) 96087878(ACET3X_007556)
ARAB: EKO05_0001181 EKO05_0007840
ZTR: MYCGRDRAFT_103966 MYCGRDRAFT_33485 MYCGRDRAFT_70208
PFJ: MYCFIDRAFT_152600 MYCFIDRAFT_27664
FFU: CLAFUR5_01012 CLAFUR5_05986
CBET: CB0940_01079 CB0940_08082
BCOM: BAUCODRAFT_285440 BAUCODRAFT_30856 BAUCODRAFT_75204
NPA: UCRNP2_1719 UCRNP2_3813
TML: GSTUM_00004829001 GSTUM_00011096001
SPO: 2543147(och1) 2543252(mnn9)
SOM: SOMG_03600(mnn9) SOMG_03933(och1)
CNE: CNA07230 CND03380 CNF01430
CNB: CNBA7030 CNBD2980 CNBF3280
CNG: CNAG_00744 CNAG_01214 CNAG_05836
CGI: CGB_A8320C CGB_D6060C CGB_F4610W
CDEU: CNBG_0783 CNBG_1188 CNBG_1711
CDEP: 91087255(L203_103044) 91089017(L203_104808)
KMG: 30163117(I203_102109) 30165543(I203_100399) 30166578(I203_100701)
KNE: 92180126(IAR55_002868) 92181533(IAR55_004275) 92184179(IAR55_006921)
TMS: TREMEDRAFT_16005 TREMEDRAFT_58229 TREMEDRAFT_61657 TREMEDRAFT_70437
TASA: A1Q1_01062 A1Q1_01267
RSX: RhiXN_01790
UMA: UMAG_02624
PFP: PFL1_01943
MLR: MELLADRAFT_27594
TPS: THAPS_264481
AAF: AURANDRAFT_66517
 » show all
Reference
1  [PMID:2644248]
  Authors
Romero PA, Herscovics A.
  Title
Glycoprotein biosynthesis in Saccharomyces cerevisiae. Characterization of alpha-1,6-mannosyltransferase which initiates outer chain formation.
  Journal
J Biol Chem 264:1946-50 (1989)
Reference
2  [PMID:1820199]
  Authors
Reason AJ, Dell A, Romero PA, Herscovics A.
  Title
Specificity of the mannosyltransferase which initiates outer chain formation in Saccharomyces cerevisiae.
  Journal
Glycobiology 1:387-91 (1991)
DOI:10.1093/glycob/1.4.387
Reference
3  [PMID:8253757]
  Authors
Nakanishi-Shindo Y, Nakayama K, Tanaka A, Toda Y, Jigami Y.
  Title
Structure of the N-linked oligosaccharides that show the complete loss of alpha-1,6-polymannose outer chain from och1, och1 mnn1, and och1 mnn1 alg3 mutants of Saccharomyces cerevisiae.
  Journal
J Biol Chem 268:26338-45 (1993)
  Sequence
[sce:YGL038C]
Reference
4  [PMID:12784644]
  Authors
Yamamoto K, Okamoto M, Yoko-o T, Jigami Y.
  Title
Salt stress induces the expression of Schizosaccharomyces pombe och1+, which encodes an initiation-specific alpha-1,6-mannosyltransferase for N-linked outer chain synthesis of cell wall mannoproteins.
  Journal
Biosci Biotechnol Biochem 67:927-9 (2003)
DOI:10.1271/bbb.67.927
  Sequence
[spo:2543147]
Reference
5  [PMID:11754484]
  Authors
Cui Z, Horecka J, Jigami Y.
  Title
Cdc4 is involved in the transcriptional control of OCH1, a gene encoding alpha-1,6-mannosyltransferase in Saccharomyces cerevisiae.
  Journal
Yeast 19:69-77 (2002)
DOI:10.1002/yea.801
Reference
6
  Authors
Tsukahara K, Watanabe T, Yoko-o T, Chigami Y.
  Title
Schizosaccharomyces pombe och1+ gene encoding alpha-1,6-mannosyltransferase and use of och1+ gene knockout fission yeast for production of glycoproteins with reduced glycosylation.
  Journal
Jpn Kokai Tokkyo Koho Koho:11 (2001)
Reference
7  [PMID:9276464]
  Authors
Nakayama K, Nakanishi-Shindo Y, Tanaka A, Haga-Toda Y, Jigami Y.
  Title
Substrate specificity of alpha-1,6-mannosyltransferase that initiates N-linked mannose outer chain elongation in Saccharomyces cerevisiae.
  Journal
FEBS Lett 412:547-50 (1997)
DOI:10.1016/S0014-5793(97)00634-0
Reference
8  [PMID:8797833]
  Authors
Suzuki A, Shibata N, Suzuki M, Saitoh F, Takata Y, Oshie A, Oyamada H, Kobayashi H, Suzuki S, Okawa Y.
  Title
Characterization of alpha-1,6-mannosyltransferase responsible for the synthesis of branched side chains in Candida albicans mannan.
  Journal
Eur J Biochem 240:37-44 (1996)
DOI:10.1111/j.1432-1033.1996.0037h.x
Reference
9  [PMID:8146181]
  Authors
Yip CL, Welch SK, Klebl F, Gilbert T, Seidel P, Grant FJ, O'Hara PJ, MacKay VL
  Title
Cloning and analysis of the Saccharomyces cerevisiae MNN9 and MNN1 genes required for complex glycosylation of secreted proteins.
  Journal
Proc Natl Acad Sci U S A 91:2723-7 (1994)
DOI:10.1073/pnas.91.7.2723
  Sequence
[sce:YPL050C]
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.232
IUBMB Enzyme Nomenclature: 2.4.1.232
ExPASy - ENZYME nomenclature database: 2.4.1.232
BRENDA, the Enzyme Database: 2.4.1.232
CAS: 346003-17-6
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