KEGG   ENZYME: 2.4.1.360
Entry
EC 2.4.1.360                Enzyme                                 
Name
2-hydroxyflavanone C-glucosyltransferase;
OsCGT
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
UDP-alpha-D-glucose:2'-hydroxy-beta-oxodihydrochalcone C6/8-beta-D-glucosyltransferase
Reaction(IUBMB)
UDP-alpha-D-glucose + a 2'-hydroxy-beta-oxodihydrochalcone = UDP + a 3'-(beta-D-glucopyranosyl)-2'-hydroxy-beta-oxodihydrochalcone [RN:R12290]
Reaction(KEGG)
R12290
Substrate
UDP-alpha-D-glucose [CPD:C00029];
2'-hydroxy-beta-oxodihydrochalcone [CPD:C22066]
Product
UDP [CPD:C00015];
3'-(beta-D-glucopyranosyl)-2'-hydroxy-beta-oxodihydrochalcone [CPD:C22067]
Comment
The enzyme has been characterized in Oryza sativa (rice), various Citrus spp., Glycine max (soybean), and Fagopyrum esculentum (buckwheat). Flavanone substrates require a 2-hydroxy group. The meta-stable flavanone substrates such as 2-hydroxynaringenin exist in an equilibrium with open forms such as 1-(4-hydroxyphenyl)-3-(2,4,6-trihydroxyphenyl)propane-1,3-dione, which are the actual substrates for the glucosyl-transfer reaction (see EC 1.14.14.162, flavanone 2-hydroxylase). The enzyme can also act on dihydrochalcones. The enzymes from citrus plants can catalyse a second C-glycosylation reaction at position 5.
History
EC 2.4.1.360 created 2018
Orthology
K23154  2-hydroxyflavanone C-glucosyltransferase
Genes
CIT102618156
CICCICLE_v10013670mg CICLE_v10031419mg
PVY116127954 116141746 116145333
MINC123206911 123215088 123215092 123217198 123224883
TCC18601595 18610301 18610303 18610304
GRA105780808
GHI107893931 107897380 107920122 121216078
GAB108459103 108462068
DZI111288214 111292515
EGR104423661 104442902 104445237 104445238 104447177 104447179 104447180 104447181 104447183
GMX100776116(UGT708D1) 100816555
GSJ114368684 114390418
PVUPHAVU_004G137900g PHAVU_004G1381000 PHAVU_004G138200g PHAVU_004G1384000
VRA106757078 106772373
VAR108344625 108344626 108344628
VUN114182225 114182226 114182227
CCAJ109792376 109792446 109792447 109792448
APRC113860191 113867818 113867999 113868048 113869104 113869105
MTRMTR_4g082810 MTR_4g082820 MTR_6g078320
CAM101494394(UGT708B1)
LJALj2g3v0604430.1(Lj2g3v0604430.1)
ADU107489286
AIP107641855 107643597
AHF112750303 112750304 112801561 112801564
LANG109330820 109340164 109340165 109341800 109356596
FVE101310443 105352450
RCN112185174 112186643 112199715
PPER18782310 18782830 18783263 18792255
PMUM103321673 103330063 103330064 103330065
PAVI110751271 110755571 110774182 110774187
MDM103410171 103416637 103417071
PXB103951017 103951080
ZJU107415869 107415922 107415924 107415925 107428877
MNT21403834 21403836 21403839 21403843
CSV101222215
CMO103489166
BHJ120080748
MCHA111019065
CMAX111482455
CMOS111458784
CPEP111777626
RCU8281269
JCU105630954 105644118 105644120
HBR110666182 110666183
MESC110609574 110623334
POP7472619 7484554
PEU105111096 105133493
PALZ118049548 118055117
QSU112009093 112012259 112012284 112022402
QLO115975566 115978863 115978864
TWL119991268 119992245
SLY101265797 101267953
SPEN107009690 107009692
SOT102596262 102597670 102601756
CANN107860487 107860489 107860490
NTA107778051 107781900
NSY104241134
NTO104092631
NAU109240202
INI109180632
ITR116012027
SIND105177295 105177356 105177357
OEU111400904
EGT105971021 105974614 105974623
SSPL121754543 121757663
ECAD122578608
CCAV112513046
DCR108196233 108226125
CSIN114256993 114269560 114296545 114308090 114308973
BVG104886914 104887295
SOE110796490
CQI110735058
NNU104598527 104603347
MING122063843 122091300
TSS122672102 122672103
PSOM113346508
NCOL116258506 116258585 116258828
OSA4340787 4340788 9266253 9270077
DOSAOs06t0288300-01(Os06g0288300) Os06t0289200-01(Os06g0289200) Os06t0289900-01(Os06g0289900)
OBR102712546 102712824 102713645 102720054
BDI100825799 100826193
ATS109732191 109732196 109764127 109764128 109782690
TDC119284006 119327435 119328551 119332597 119336082 119336186 119340053 119341887 119348704 119348705
TAES123060189 123094271 123094282 123094294 123137106 123150761 123153518 123154251 123158939 123159486 123159778 123165825 123166487 123166980 123182377
SBI8066139 8073750 8076408 8076410
ZMA100191917 100194125 100217220 100281608 100282556
SITA101781599 101782011
SVS117851226 117851227 117851257
PVIR120643951 120684770 120703361
PHAI112888580 112889866 112889867
PDA103708718 103716468 120111946
EGU105049993 105050648 105060740
MUS103974397
DCT110094296 110097095 110103872 110116333
PEQ110031195 110031196 110031197 110037294
AOF109849013
ATR18438457
 » show all
Reference
1  [PMID:19411659]
  Authors
Brazier-Hicks M, Evans KM, Gershater MC, Puschmann H, Steel PG, Edwards R
  Title
The C-glycosylation of flavonoids in cereals.
  Journal
J Biol Chem 284:17926-34 (2009)
DOI:10.1074/jbc.M109.009258
  Sequence
[osa:9266253]
Reference
2  [PMID:25142187]
  Authors
Nagatomo Y, Usui S, Ito T, Kato A, Shimosaka M, Taguchi G
  Title
Purification, molecular cloning and functional characterization of flavonoid C-glucosyltransferases from Fagopyrum esculentum M. (buckwheat) cotyledon.
  Journal
Plant J 80:437-48 (2014)
DOI:10.1111/tpj.12645
  Sequence
Reference
3  [PMID:25979175]
  Authors
Hirade Y, Kotoku N, Terasaka K, Saijo-Hamano Y, Fukumoto A, Mizukami H
  Title
Identification and functional analysis of 2-hydroxyflavanone C-glucosyltransferase in soybean (Glycine max).
  Journal
FEBS Lett 589:1778-86 (2015)
DOI:10.1016/j.febslet.2015.05.010
  Sequence
[gmx:100776116]
Reference
4  [PMID:28370711]
  Authors
Ito T, Fujimoto S, Suito F, Shimosaka M, Taguchi G
  Title
C-Glycosyltransferases catalyzing the formation of di-C-glucosyl flavonoids in citrus plants.
  Journal
Plant J 91:187-198 (2017)
DOI:10.1111/tpj.13555
  Sequence
[cit:102618156]
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.360
IUBMB Enzyme Nomenclature: 2.4.1.360
ExPASy - ENZYME nomenclature database: 2.4.1.360
BRENDA, the Enzyme Database: 2.4.1.360
LinkDB

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