KEGG   ENZYME: 2.4.1.65
Entry
EC 2.4.1.65                 Enzyme                                 
Name
3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase;
(Lea)-dependent (alpha-3/4)-fucosyltransferase;
alpha(1,3/1,4) fucosyltransferase III;
alpha-(1->4)-L-fucosyltransferase;
alpha-4-L-fucosyltransferase;
beta-acetylglucosaminylsaccharide fucosyltransferase;
FucT-II;
Lewis alpha-(1->3/4)-fucosyltransferase;
Lewis blood group alpha-(1->3/4)-fucosyltransferase;
Lewis(Le) blood group gene-dependent alpha-(1->3/4)-L-fucosyltransferase;
blood group Lewis alpha-4-fucosyltransferase;
blood-group substance Lea-dependent fucosyltransferase;
guanosine diphosphofucose-beta-acetylglucosaminylsaccharide 4-alpha-L-fucosyltransferase;
guanosine diphosphofucose-glycoprotein 4-alpha-L-fucosyltransferase;
guanosine diphosphofucose-glycoprotein 4-alpha-fucosyltransferase;
3-alpha-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase;
GDP-beta-L-fucose:3-beta-D-galactosyl-N-acetyl-D-glucosaminyl-R 4I-alpha-L-fucosyltransferase;
GDP-L-fucose:3-beta-D-galactosyl-N-acetyl-D-glucosaminyl-R 4I-alpha-L-fucosyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
GDP-beta-L-fucose:beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-R 4I-alpha-L-fucosyltransferase (configuration-inverting)
Reaction(IUBMB)
GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-R = GDP + beta-D-galactosyl-(1->3)-[alpha-L-fucosyl-(1->4)]-N-acetyl-beta-D-glucosaminyl-R [RN:R04553]
Reaction(KEGG)
R04553 > R06155(G);
(other) R06025(G) R06038(G) R06039(G) R06075(G) R06076(G) R06095(G) R06162(G) R06163(G) R06164(G) R06165(G) R06221(G) R06222(G) R06224(G) R06227(G) R06230(G) R13173(G) R13174(G)
Substrate
GDP-beta-L-fucose [CPD:C00325];
beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-R [CPD:C04656]
Product
GDP [CPD:C00035];
beta-D-galactosyl-(1->3)-[alpha-L-fucosyl-(1->4)]-N-acetyl-beta-D-glucosaminyl-R [CPD:C04850]
Comment
This enzyme is the product of the Lewis blood group gene. Normally acts on a glycoconjugate where R (see reaction) is a glycoprotein or glycolipid. Although it is a 4-fucosyltransferase, it has a persistent 3-fucosyltransferase activity towards the glucose residue in free lactose. This enzyme fucosylates on O-4 of an N-acetylglucosamine that carries a galactosyl group on O-3, unlike EC 2.4.1.152, 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase, which fucosylates on O-3 of an N-acetylglucosamine that carries a galactosyl group on O-4. Enzymes catalysing the 4-alpha-fucosylation of the GlcNAc in beta-D-Gal-(1->3)-beta-GlcNAc sequences (with some activity also as 3-alpha-fucosyltransferases) are present in plants, where the function in vivo is the modification of N-glycans. In addition, the fucTa gene of Helicobacter strain UA948 encodes a fucosyltransferase with both 3-alpha- and 4-alpha-fucosyltransferase activities.
History
EC 2.4.1.65 created 1972, modified 2001, modified twice 2002
Pathway
ec00513  Various types of N-glycan biosynthesis
ec00601  Glycosphingolipid biosynthesis - lacto and neolacto series
ec01100  Metabolic pathways
Orthology
K00716  galactoside 3(4)-fucosyltransferase 3
K07633  galactoside alpha-1,3-fucosyltransferase 5
K07634  galactoside alpha-1,3-fucosyltransferase 6
K14412  alpha-1,4-fucosyltransferase
K20151  alpha(1,3/1,4) fucosyltransferase
Genes
HSA: 2525(FUT3) 2527(FUT5) 2528(FUT6)
PTR: 455628(FUT5) 493975(FUT3) 493976(FUT6)
PPS: 100980873(FUT6) 100982106(FUT5) 100982431(FUT3)
GGO: 101153495(FUT6) 129523557(FUT5) 129528860(FUT3)
PON: 100431975(FUT6) 100432841(FUT3) 100433605 112130139
PPYG: 129019800 129019801 129019802 129020757(FUT6)
NLE: 100591958 115830897
HMH: 116465982(FUT5)
SSYN: 129466403 129466458
MCC: 106994615 697452 697582
MCF: 102144516 102144879(FUT5) 102145756
MTHB: 126942684 126942687 126942688 126942689
MNI: 105484872 105484878 105487104
CSAB: 103233750 103233751(FUT5)
CATY: 105584486 105591841 105591842 105591843
PANU: 101024201 101025270
TGE: 112612385 112613050
MLEU: 105530055 105530058
RRO: 104665387 104665388 104665389
RBB: 108526239
TFN: 117075725 117075726 117075727
PTEH: 111532485 111534551
CANG: 105512866 105512868 105512870
SBQ: 101046574 101046882
CIMI: 108304247 108304248
ANAN: 135276672
CSYR: 103250103 103266005 103269908
LCAT: 123636469
PCOQ: 105811901
MAUA: 121136969 121136970
PLEU: 114688854
MFOT: 126490367
NGI: 103737200
HGL: 101708404
CPOC: 100723961 100724236 111755640
DORD: 105990300
DSP: 122122711 122122712 122122713 122122847
NCAR: 124969249
MMMA: 107135039
OPI: 101523558
GVR: 103590759
CLUD: 112666354 125752087
VLG: 121495871 121495997
AML: 100468460
UAR: 123779029
ZCA: 113916121
FCA: 101093666
PYU: 121021423
PCOO: 112853013
PBG: 122486768
LRUF: 124523120
PTG: 102972414(FUT3)
PPAD: 109256005(FUT6)
PUC: 125930957
AJU: 106975444
BTA: 338077(FUT6)
BOM: 102271549(FUT6)
BBIS: 104979717
CHX: 102181295
OAS: 101108745
BTAX: 128051431
ODA: 120864866
CCAD: 122440278
MBEZ: 129559035(FUT6)
SSC: 100513844
CFR: 102504974
CBAI: 105078245
VPC: 102528105
BACU: 103005588(FUT3)
LVE: 103076464(FUT3)
OOR: 101276278
PCAD: 102982037
ECB: 111774191
EAI: 106832782
MYB: 102255875(FUT3)
MLF: 102420280
PDIC: 114503625
PHAS: 123813905
MMF: 118643893(FUT6)
PGIG: 120615429
PVP: 105301069
RAY: 107510971
MJV: 108409216
LAV: 100675894
DNM: 101445841
MDO: 100015438(FUT3)
GAS: 123232125(FUT6)
SHR: 100927060(FUT6)
AFZ: 127541279
OAA: 100092622 100680766
GGA: 428341(FUT6)
PCOC: 116236122
MGP: 100544367(FUT6)
CJO: 107325644
TPAI: 128090365
LMUT: 125684796
NMEL: 110388979
APLA: 119714238
ACYG: 106048410
CATA: 118260536
AFUL: 116499200(FUT6)
CCW: 104697412
CBRC: 103621204
ACHL: 103799887
HRT: 120763300
SATI: 136371785
FPG: 101918531(FUT3)
FCH: 102058652(FUT3)
CCRI: 104163030
NNT: 104411597
SHAB: 115618176(FUT6)
ACUN: 113489486
TALA: 104369415
ACHC: 115349308
HALD: 104313889
HLE: 104828543
AGEN: 126041904
GCL: 127025605
CSTI: 104553666
LDI: 104347617
MNB: 103768775
DPUB: 104303242
AVIT: 104275898
BRHI: 104501369
EGZ: 104124394
NNI: 104018761
PCRI: 104039042
PCAO: 104052442
PADL: 103916323
AFOR: 103900456(FUT6)
FGA: 104083579
GSTE: 104259264
CLV: 102095283
MUI: 104537212
PGUU: 104467640
PLET: 104625518
EHS: 104507394
CMAC: 104476986
CUCA: 104054859
TEO: 104378934
BREG: 104641470
OHA: 104330851
ACAR: 104529800
CPEA: 104395831
CVF: 104290077
RTD: 128900549
AAM: 106492204
AROW: 112972303 112972318
NPD: 112949093 112949106
TGT: 104572962
DNE: 112989580 112989581(FUT6)
SCAM: 104145769
ASN: 102376263 102376514
AMJ: 102561627(FUT3)
PSS: 102456334 102456585 102456836
CMY: 102942494(FUT3)
CCAY: 125627272
DCC: 119848411
CPIC: 101941134
TST: 117868969
CABI: 116839965
MRV: 120391734 120391735
ACS: 100565215
ASAO: 132781752
PVT: 110072709
SUND: 121918407 121918790 121930530 121936493 121936504 121936505 121937082
PBI: 103063308
VKO: 123017603 123017617
HCG: 128343779 128343780 128343781 128345741 128346266
GJA: 107123994
EMC: 129330905 129335764
XLA: 399049(fut6.S)
XTR: 100495376(fut6)
RTEM: 120916902 120917240
BBUF: 120980031 120980032 120980509 120981056
BGAR: 122920039 122920040 122920313 122920813 122920847
MUO: 115468955
GSH: 117352259
LCM: 102363620
SCLV: 120328347 120333053 120333431 120342992 120343163
LPIC: 129255115 129272263
LSIN: 126979003
BTAB: 109031361
HAME: 121876812
PCLA: 123760325 123760329 123772962
PVUL: 126807894 126815293 126815484 126815576 126820759
HRF: 124130910 124130912
HRJ: 124277798 124283392
OED: 125662413
PMAX: 117336925
RPHI: 132731383
DPOL: 127841082 127842686 127857225 127862126
MAEA: 128209686 128226411
ATEN: 116308437
ATH: AT1G71990(FUT13)
ALY: 9323469
CRB: 17895957
CSAT: 104701862 104712777 104750248 104751043
EUS: EUTSA_v10018642mg EUTSA_v10027318mg
BRP: 103831713
BNA: 106354190 106376816
BOE: 106298421
RSZ: 108823850
THJ: 104809738
CPAP: 110813288
CIT: 102620994
CIC: CICLE_v10001278mg
PVY: 116108449
MINC: 123228552
TCC: 18592964
GRA: 105783399
GHI: 107895104 107920235
GAB: 108464570
HSYR: 120162049 120164451
DZI: 111276738
EGR: 104449507
GMX: 100791990 100792111 100792300 100792965
GSJ: 114369682 114383657 114390393 114403056
PVU: PHAVU_007G088300g
VRA: 106770892
VAR: 108324168
VUN: 114192745
VUM: 124821549
CCAJ: 109812205
APRC: 113872060
MTR: 25484992
TPRA: 123918603
CAM: 101495537
PSAT: 127082297 127083445 127092282 127093397 127095215 127128743 127131137
VVO: 131643685
LJA: 130718937
ADU: 107467776
AIP: 107619064
AHF: 112712527 112776158
LANG: 109335938
PCIN: 129289389 129292529 129306218
QSA: O6P43_004076
FVE: 101297402
RCN: 112179704
PPER: 18793901
PMUM: 103319172
PAVI: 110767076
PDUL: 117614862
MDM: 103425331 103428943 103441831
MSYL: 126604202 126631936
PXB: 103952576 103967942
ZJU: 107421412
MNT: 21390266
CSAV: 115702356
CSV: 101216230
CMO: 103496555
BHJ: 120075937
MCHA: 111006550
CMAX: 111487250 111488938
CMOS: 111445526 111459313
CPEP: 111800559 111808579
RCU: 8262699
MEAN: 126662407
JCU: 105646728
HBR: 110649282
MESC: 110609616
POP: 18104441 7467417
PEU: 105110615
PALZ: 118056492
JRE: 108991374 109011840
CILL: 122316368 122319016
CAVE: 132190595
QSU: 112022772
QLO: 115975387
TWL: 119992883
VVI: 100256631
VRI: 117907734
SLY: 543777(FUT13)
SPEN: 107021151
SOT: 102590542
SSTN: 125872655
SDUL: 129901234
CANN: 107872770
LBB: 132644173
NTA: 107766558 107823850
NSY: 104229361
NTO: 104097007
NAU: 109227310
INI: 109193282(a4Lea)
ITR: 116021144
SIND: 105159546 105175757
OEU: 111379076 111386235
EGT: 105968236 105974235
SSPL: 121753977 121756999 121805472
SMIL: 131020489
SHIS: 125192317 125218992
APAN: 127257182
HAN: 110889492
ECAD: 122588469
LSV: 111918717
CCAV: 112503151
DCR: 108208636 108211012 108214760
CSIN: 114273423
RVL: 131300078 131329567
AEW: 130776431
BVG: 104894700
SOE: 110787629
CQI: 110714072 110731684
ATRI: 130811016
MOF: 131161697
NNU: 104596822
MING: 122062782 122065742 122090638
TSS: 122656864 122662437
PSOM: 113306813 113356155
OSA: 4351626 9270041
OBR: 102714333 102720232
OGL: 127756925 127771416
BDI: 100828844
ATS: 109752008
TDC: 119302331 119308107
TAES: 123102927 123111028 123120087
TUA: 125508416
LPER: 127299686
LRD: 124653465
SBI: 8076283
ZMA: 732742
MFLO: 136504885 136505220 136508552 136509061
SITA: 101757637
SVS: 117865888
PVIR: 120663707 120696833
PHAI: 112883882
PDA: 103701280
EGU: 105051880
MUS: 103985213 103988951 103989072 103989073 103989074
ZOF: 122032165 122034617
DCT: 110098286
PEQ: 110030264
AOF: 109824399
MSIN: 131234889
NCOL: 116258499
ATR: 18436672
SMO: SELMODRAFT_412633(FucTB)
PPP: 112295359
GTT: GUITHDRAFT_122340
PTD: PTET_a0498
AGZ: M0C34_01580
MEEF: JWZ97_16685
FGU: SD28_06620
HMAR: HVMH_0588
MARD: IBG28_16530
AJP: AMJAP_0620
EBH: BSEPE_0695
PNU: Pnuc_0251
BPSI: IX83_03990
SLT: Slit_2890
NIM: W01_02790
RBE: RBE_0503
RBO: A1I_02895
AZL: AZL_007090
ALI: AZOLI_0610
ABS: AZOBR_p1120031
ABQ: ABAZ39_20530
ABF: AMK58_20385
ATI: AL072_00805
AHU: A6A40_02580
AZT: TSH58p_24720
AZM: DM194_02250
AOZ: HUE56_20210
AARE: D3093_21945
AZS: E6C72_07530
MAGX: XM1_1803
MAGN: WV31_16795
HPY: HP_0379 HP_0651
HEO: C694_01925 C694_03365
HPJ: jhp_0596(fucT) jhp_1002(fucU)
HPA: HPAG1_0636 HPAG1_1013
HPS: HPSH_03605 HPSH_05520
HHP: HPSH112_03760 HPSH112_05330
HHQ: HPSH169_03340 HPSH169_05305
HHR: HPSH417_03150 HPSH417_05070
HPG: HPG27_1018 HPG27_613
HPP: HPP12_0664(fucT) HPP12_1042
HPB: HELPY_0720(futB) HELPY_1046(futA)
HPL: HPB8_427(fucT1) HPB8_854(fucT7)
HPC: HPPC_03535 HPPC_05220
HCA: HPPC18_03150 HPPC18_05315
HPM: HPSJM_03315 HPSJM_05295
HPE: HPELS_01320 HPELS_03280
HPO: HMPREF4655_20949 HMPREF4655_21266
HPI: hp908_0664(fucT)
HPQ: hp2017_0641(fucT)
HPW: hp2018_0642(fucT)
HPU: HPCU_03535 HPCU_05450
HEF: HPF16_0660(fucT) HPF16_1018(fucT)
HPF: HPF30_0312(fucT) HPF30_0677(fucT)
HEQ: HPF32_0329(fucT) HPF32_0668(fucT)
HEX: HPF57_0674(fucT) HPF57_1038(fucT)
HPT: HPSAT_03535 HPSAT_05130
HPZ: HPKB_0693 HPKB_1004
HPV: HPV225_0659
HPX: HMPREF0462_0710 HMPREF0462_1089
HEN: HPSNT_03355
HPH: HPLT_03250 HPLT_05320
HEG: HPGAM_03360 HPGAM_05520
HPN: HPIN_03385
HEP: HPPN120_03460 HPPN120_05205
HEU: HPPN135_03215 HPPN135_05490
HES: HPSA_03080 HPSA_05205
HCN: HPB14_03415 HPB14_05015
HPD: KHP_0670(fucTa) KHP_0977(fucTb)
HEY: MWE_0857 MWE_1252
HER: C695_01925 C695_03370
HEI: C730_01925 C730_03370
HPYA: HPAKL117_03085 HPAKL117_05025
HPYK: HPAKL86_04810 HPAKL86_05545
HPYO: HPOK113_0665 HPOK113_1036
HPYL: HPOK310_0645 HPOK310_0973
HPYB: HPOKI102_03755 HPOKI102_05640
HPYC: HPOKI112_03785 HPOKI112_05675
HPYD: HPOKI128_05060
HPYE: HPOKI154_03330 HPOKI154_05345
HPYF: HPOKI422_03760 HPOKI422_05665
HPYG: HPOKI673_03310 HPOKI673_05330
HPYH: HPOKI828_03320 HPOKI828_05335
HPYJ: HPOKI898_03755 HPOKI898_05650
HPYR: K747_05675 K747_07345
HPYI: K750_05570
HPYU: K751_02280 K751_04125
HPYM: K749_00370 K749_01980
HEM: K748_00470 K748_06920
HEB: U063_0955 U063_1387
HEZ: U064_0959 U064_1392
HHE: HH_1776
HAC: Hac_1063(fuc)
HFE: HFELIS_11760
HBI: HBZC1_11830
HCE: HCW_04425
HCM: HCD_06740
HSH: NHP194022_13850
HAIL: ASB7_00490 ASB7_03660
SUA: Saut_1498
SULR: B649_10005
ADE: Adeh_3056
ACP: A2cp1_3255
BDQ: CIK05_07105
BHU: bhn_I0452
PFAC: PFJ30894_01738
PMC: P9515_13921
SNG: SNE_A13120
CAA: Caka_2548
AMU: Amuc_0760
AMAK: J5W79_06255
VEG: SAMN05444156_1977
LPAV: PLANPX_5568
PLM: Plim_1042
HPRI: P0082_11105
BHY: BHWA1_00146
BHD: BHYOB78_02265
BHP: BHAMNSH16_01940
BFS: BF9343_3370
BNO: K8P02_19270
PPN: Palpr_1132 Palpr_1136
COPR: Cop2CBH44_18030
CPOL: WJU16_17295
LACS: H4075_02030
FLP: LK994_12525
PHE: Phep_0149
PCM: AY601_4410
PEJ: FYC62_14360
PROE: H9L23_20465
MUC: MuYL_4908
MMAB: HQ865_11965
ALM: AO498_13665
ALGO: GYM62_04035
HQI: H9L05_02385
PONT: C1N53_07325
FFA: FFWV33_07715
FNK: E1750_13960
CAPF: FOC45_00305
KDI: Krodi_2047
AFLA: FHG64_04105
MRU: mru_1527
MEL: Metbo_0771
 » show all
Reference
1  [PMID:7287719]
  Authors
Prieels JP, Monnom D, Dolmans M, Beyer TA, Hill RL.
  Title
Co-purification of the Lewis blood group N-acetylglucosaminide alpha 1 goes to 4 fucosyltransferase and an N-acetylglucosaminide alpha 1 goes to 3 fucosyltransferase from human milk.
  Journal
J Biol Chem 256:10456-63 (1981)
Reference
2  [PMID:10671538]
  Authors
Rasko DA, Wang G, Palcic MM, Taylor DE
  Title
Cloning and characterization of the alpha(1,3/4) fucosyltransferase of Helicobacter pylori.
  Journal
J Biol Chem 275:4988-94 (2000)
DOI:10.1074/jbc.275.7.4988
  Sequence
[ag:AAF35291]
Reference
3  [PMID:12084979]
  Authors
Wilson IB
  Title
Identification of a cDNA encoding a plant Lewis-type alpha1,4-fucosyltransferase.
  Journal
Glycoconj J 18:439-47 (2001)
DOI:10.1023/A:1016030000527
  Sequence
[sly:543777]
Reference
4  [PMID:12676935]
  Authors
Ma B, Wang G, Palcic MM, Hazes B, Taylor DE
  Title
C-terminal amino acids of Helicobacter pylori alpha1,3/4 fucosyltransferases determine type I and type II transfer.
  Journal
J Biol Chem 278:21893-900 (2003)
DOI:10.1074/jbc.M301704200
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.65
IUBMB Enzyme Nomenclature: 2.4.1.65
ExPASy - ENZYME nomenclature database: 2.4.1.65
BRENDA, the Enzyme Database: 2.4.1.65
CAS: 37277-69-3
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