Entry |
|
Name |
dolichyl-diphosphooligosaccharide---protein glycotransferase;
dolichyldiphosphooligosaccharide-protein glycosyltransferase;
asparagine N-glycosyltransferase;
dolichyldiphosphooligosaccharide-protein oligosaccharyltransferase;
dolichylpyrophosphodiacetylchitobiose-protein glycosyltransferase;
oligomannosyltransferase;
oligosaccharide transferase;
dolichyldiphosphoryloligosaccharide-protein oligosaccharyltransferase;
dolichyl-diphosphooligosaccharide:protein-L-asparagine oligopolysaccharidotransferase;
STT3
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Class |
Transferases;
Glycosyltransferases;
Transferring other glycosyl groups
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Sysname |
dolichyl-diphosphooligosaccharide:protein-L-asparagine N-beta-D-oligopolysaccharidotransferase
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Reaction(IUBMB) |
dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine [RN: R04216]
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Reaction(KEGG) |
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Substrate |
dolichyl diphosphooligosaccharide [CPD: C04213];
[protein]-L-asparagine
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Product |
dolichyl diphosphate [CPD: C00621];
glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine
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Comment |
Occurs in eukaryotes that form a glycoprotein by the transfer of a glucosyl-mannosyl-glucosamine polysaccharide to the side-chain of an L-asparagine residue in the sequence -Asn-Xaa-Ser- or -Asn-Xaa-Thr- (Xaa not Pro) in nascent polypeptide chains. The basic oligosaccharide is the tetradecasaccharide Glc3Man9GlcNAc2 (for diagram {polysacc/Dol14}). However, smaller oligosaccharides derived from it and oligosaccharides with additional monosaccharide units attached may be involved. See ref [2] for a review of N-glycoproteins in eukaryotes. Man3GlcNAc2 seems to be common for all of the oligosaccharides involved with the terminal N-acetylglucosamine linked to the protein L-asparagine. Occurs on the cytosolic face of the endoplasmic reticulum. The dolichol involved normally has 14-21 isoprenoid units with two trans double-bonds at the omega end, and the rest of the double-bonds in cis form.
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History |
EC 2.4.99.18 created 1984 as EC 2.4.1.119, transferred 2012 to EC 2.4.99.18
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Pathway |
ec00513 | Various types of N-glycan biosynthesis |
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Orthology |
K07151 | dolichyl-diphosphooligosaccharide---protein glycosyltransferase |
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Genes |
» show all
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Reference |
|
Authors |
Das RC, Heath EC. |
Title |
Dolichyldiphosphoryloligosaccharide--protein oligosaccharyltransferase; solubilization, purification, and properties. |
Journal |
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Reference |
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Authors |
Song W, Henquet MG, Mentink RA, van Dijk AJ, Cordewener JH, Bosch D, America AH, van der Krol AR |
Title |
N-glycoproteomics in plants: perspectives and challenges. |
Journal |
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Other DBs |
ExPASy - ENZYME nomenclature database: | 2.4.99.18 |
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LinkDB |
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