Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
BRITE hierarchy

S-adenosyl-L-methionine:L-histidine-[translation elongation factor 2] 2-[(3S)-3-amino-3-carboxypropyl]transferase

S-adenosyl-L-methionine + L-histidine-[translation elongation factor 2] = S-methyl-5'-thioadenosine + 2-[(3S)-3-amino-3-carboxypropyl]-L-histidine-[translation elongation factor 2] [RN:R10455]

R10455
Reaction

S-adenosyl-L-methionine [CPD:C00019];
L-histidine-[translation elongation factor 2] [CPD:C20663]

A [4Fe-4S] enzyme that modifies a histidine residue of the translation elongation factor 2 (EF2) via a 3-amino-3-carboxypropyl radical. The enzyme is present in archae and eukaryotes but not in eubacteria. The enzyme is a member of the 'AdoMet radical' (radical SAM) family and generates the 3-amino-3-carboxypropyl radical by an uncanonical clevage of S-adenosyl-L-methionine. The relevant histidine of EF2 is His715 in mammals, His699 in yeast and His600 in Pyrococcus horikoshii. Part of diphthamide biosynthesis.

EC 2.5.1.108 created 2013

1  [PMID:15485916]

Liu S, Milne GT, Kuremsky JG, Fink GR, Leppla SH

Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2.

Mol Cell Biol 24:9487-97 (2004)
DOI:10.1128/MCB.24.21.9487-9497.2004

[mmu:116905] [sce:YIL103W]

2  [PMID:20559380]

Zhang Y, Zhu X, Torelli AT, Lee M, Dzikovski B, Koralewski RM, Wang E, Freed J, Krebs C, Ealick SE, Lin H

Diphthamide biosynthesis requires an organic radical generated by an iron-sulphur enzyme.

Nature 465:891-6 (2010)
DOI:10.1038/nature09138

[pho:PH1105]

3  [PMID:20931132]

Zhu X, Dzikovski B, Su X, Torelli AT, Zhang Y, Ealick SE, Freed JH, Lin H

Mechanistic understanding of Pyrococcus horikoshii Dph2, a [4Fe-4S] enzyme required for diphthamide biosynthesis.

Mol Biosyst 7:74-81 (2011)
DOI:10.1039/c0mb00076k

[pho:PH1105]

4  [PMID:27465315]

Dong M, Horitani M, Dzikovski B, Pandelia ME, Krebs C, Freed JH, Hoffman BM, Lin H

Organometallic Complex Formed by an Unconventional Radical S-Adenosylmethionine Enzyme.

J Am Chem Soc 138:9755-8 (2016)
DOI:10.1021/jacs.6b04155