KEGG   ENZYME: 2.5.1.25
Entry
EC 2.5.1.25                 Enzyme                                 

Name
tRNA-uridine aminocarboxypropyltransferase;
S-adenosyl-L-methionine:tRNA-uridine 3-(3-amino-3-carboxypropyl)transferase;
tapT (gene name);
DTWD1 (gene name);
DTWD2 (gene name);
S-adenosyl-L-methionine:uridine47 in tRNAPhe 3-[(3S)-3-amino-3-carboxypropyl]transferase
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
Sysname
S-adenosyl-L-methionine:uridine in tRNA 3-[(3S)-3-amino-3-carboxypropyl]transferase
Reaction(IUBMB)
S-adenosyl-L-methionine + a uridine in tRNA = S-methyl-5'-thioadenosine + a 3-[(3S)-3-amino-3-carboxypropyl]uridine in tRNA [RN:R03019]
Reaction(KEGG)
R03019
Substrate
S-adenosyl-L-methionine [CPD:C00019];
uridine in tRNA
Product
S-methyl-5'-thioadenosine [CPD:C00170];
3-[(3S)-3-amino-3-carboxypropyl]uridine in tRNA
Comment
3-[(3S)-3-amino-3-carboxypropyl]uridine (acp3U) is a highly conserved modification found in tRNA core region in bacteria and eukaryotes that confers thermal stability on tRNA. The enzyme from the bacterium Escherichia coli catalyses the modification of uridine47 in the V-loop of tRNAs for Arg2, Ile1, Ile2, Ile2v, Lys, Met, Phe, Val2A, and Val2B. The human homologs DTWD1 and DTWD2 are responsible for acp3U formation at positions 20 and 20a, respectively, in the D-loop of several cytoplasmic tRNAs.
History
EC 2.5.1.25 created 1984, modified 2014, modified 2020
Reference
1  [PMID:4597321]
  Authors
Nishimura S, Taya Y, Kuchino Y, Oashi Z.
  Title
Enzymatic synthesis of 3-(3-amino-3-carboxypropyl)uridine in Escherichia coli phenylalanine transfer RNA: transfer of the 3-amino-acid-3-carboxypropyl group from S-adenosylmethionine.
  Journal
Biochem Biophys Res Commun 57:702-8 (1974)
DOI:10.1016/0006-291X(74)90603-2
Reference
2  [PMID:31804502]
  Authors
Takakura M, Ishiguro K, Akichika S, Miyauchi K, Suzuki T.
  Title
Biogenesis and functions of aminocarboxypropyluridine in tRNA.
  Journal
Nat Commun 10:5542 (2019)
DOI:10.1038/s41467-019-13525-3
Reference
3  [PMID:31863583]
  Authors
Meyer B, Immer C, Kaiser S, Sharma S, Yang J, Watzinger P, Weiss L, Kotter A, Helm M, Seitz HM, Kotter P, Kellner S, Entian KD, Wohnert J.
  Title
Identification of the 3-amino-3-carboxypropyl (acp) transferase enzyme responsible for acp3U formation at position 47 in Escherichia coli tRNAs.
  Journal
Nucleic Acids Res 48:1435-1450 (2020)
DOI:10.1093/nar/gkz1191
Other DBs
ExplorEnz - The Enzyme Database: 2.5.1.25
IUBMB Enzyme Nomenclature: 2.5.1.25
ExPASy - ENZYME nomenclature database: 2.5.1.25
BRENDA, the Enzyme Database: 2.5.1.25
LinkDB

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