| Entry |
|
|---|
| Name |
protein geranylgeranyltransferase type II;
GGTaseII;
Rab geranylgeranyltransferase;
RabGGTase;
geranylgeranyl-diphosphate,geranylgeranyl-diphosphate:protein-cysteine geranyltransferase
|
|---|
| Class |
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
|
|---|
| Sysname |
geranylgeranyl-diphosphate:protein-cysteine geranyltransferase
|
|---|
| Reaction(IUBMB) |
geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate
|
|---|
| Substrate |
geranylgeranyl diphosphate [CPD: C00353];
protein-cysteine [CPD: C20119]
|
|---|
| Product |
S-geranylgeranyl-protein;
diphosphate [CPD: C00013]
|
|---|
| Comment |
This enzyme, along with protein farnesyltransferase (EC 2.5.1.58) and protein geranylgeranyltransferase type I (EC 2.5.1.59), constitutes the protein prenyltransferase family of enzymes. Attaches geranylgeranyl groups to two C-terminal cysteines in Ras-related GTPases of a single family, the Rab family (Ypt/Sec4 in lower eukaryotes) that terminate in XXCC, XCXC and CCXX motifs. Reaction is entirely dependent on the Rab substrate being bound to Rab escort protein (REP). Post-translational modification with the geranylgeranyl moiety is essential for Rab GTPases to be able to control the processes of membrane docking and fusion [5].
|
|---|
| History |
EC 2.5.1.60 created 2003
|
|---|
| Orthology |
| K05956 | geranylgeranyl transferase type-2 subunit beta |
| K14050 | geranylgeranyl transferase type-2 subunit alpha |
|
|---|
| Genes |
» show all
|
|---|
| Reference |
|
|---|
| Authors |
Casey PJ, Seabra MC. |
|---|
| Title |
Protein prenyltransferases. |
|---|
| Journal |
|
|---|
| Reference |
|
|---|
| Authors |
Wilson AL, Erdman RA, Castellano F, Maltese WA. |
|---|
| Title |
Prenylation of Rab8 GTPase by type I and type II geranylgeranyl transferases. |
|---|
| Journal |
|
|---|
| Reference |
|
|---|
| Authors |
Zhang H, Seabra MC, Deisenhofer J. |
|---|
| Title |
Crystal structure of Rab geranylgeranyltransferase at 2.0 A resolution. |
|---|
| Journal |
|
|---|
| Sequence |
|
|---|
| Reference |
|
|---|
| Authors |
Thoma NH, Niculae A, Goody RS, Alexandrov K. |
|---|
| Title |
Double prenylation by RabGGTase can proceed without dissociation of the mono-prenylated intermediate. |
|---|
| Journal |
|
|---|
| Reference |
|
|---|
| Authors |
Rak A, Niculae A, Kalinin A, Thoma NH, Sidorovitch V, Goody RS, Alexandrov K. |
|---|
| Title |
In vitro assembly, purification, and crystallization of the rab geranylgeranyl transferase:substrate complex. |
|---|
| Journal |
|
|---|
| Reference |
6 |
|---|
| Authors |
Gibbs, R.A. |
|---|
| Title |
Prenyl transfer and the enzymes of terpenoid and steroid biosynthesis. |
|---|
| Journal |
In: Sinnott, M. (Ed.), Comprehensive Biological Catalysis. A Mechanistic Reference, vol. 1, Academic Press, San Diego, CA, 1998, p. 31-118. |
|---|
| Other DBs |
| ExplorEnz - The Enzyme Database: | 2.5.1.60 |
| ExPASy - ENZYME nomenclature database: | 2.5.1.60 |
|
|---|
| LinkDB |
|
|---|
