Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
BRITE hierarchy

geranylgeranyl-diphosphate:isopentenyl-diphosphate transtransferase (adding 1 isopentenyl unit)

geranylgeranyl diphosphate + isopentenyl diphosphate = (2E,6E,10E,14E)-geranylfarnesyl diphosphate + diphosphate [RN:R07475]

R07475
Reaction

geranylgeranyl diphosphate [CPD:C00353];
isopentenyl diphosphate [CPD:C00129]

The enzyme from Methanosarcina mazei is involved in biosynthesis of the polyprenyl side-chain of methanophenazine, an electron carrier utilized for methanogenesis. It prefers geranylgeranyl diphosphate and farnesyl diphosphate as allylic substrate [1]. The enzyme from Aeropyrum pernix prefers farnesyl diphosphate as allylic substrate. The enzyme is involved in the biosynthesis of C25-C25 membrane lipids [2].

EC 2.5.1.81 created 2010

1  [PMID:20097171]

Ogawa T, Yoshimura T, Hemmi H

Geranylfarnesyl diphosphate synthase from Methanosarcina mazei: Different role, different evolution.

Biochem Biophys Res Commun 393:16-20 (2010)
DOI:10.1016/j.bbrc.2010.01.063

[mma:MM_0789]

2  [PMID:10632701]

Tachibana A, Yano Y, Otani S, Nomura N, Sako Y, Taniguchi M

Novel prenyltransferase gene encoding farnesylgeranyl diphosphate synthase from a hyperthermophilic archaeon, Aeropyrum pernix. Molecularevolution with alteration in product specificity.

Eur J Biochem 267:321-8 (2000)
DOI:10.1046/j.1432-1327.2000.00967.x

[ag:BAA88983]

3  [PMID:8137956]

Tachibana A

A novel prenyltransferase, farnesylgeranyl diphosphate synthase, from the haloalkaliphilic archaeon, Natronobacterium pharaonis.

FEBS Lett 341:291-4 (1994)
DOI:10.1016/0014-5793(94)80475-3

[nph:NP_3696A]

4  [PMID:15548566]

Lee PC, Mijts BN, Petri R, Watts KT, Schmidt-Dannert C

Alteration of product specificity of Aeropyrum pernix farnesylgeranyl diphosphate synthase (Fgs) by directed evolution.

Protein Eng Des Sel 17:771-7 (2004)
DOI:10.1093/protein/gzh089

[ag:BAA88983]