| Entry |
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| Name |
ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific];
RER2;
Rer2p;
Rer2p Z-prenyltransferase;
Srt1p;
Srt2p Z-prenyltransferase;
ACPT;
dehydrodolichyl diphosphate synthase 1
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| Class |
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
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| Sysname |
(2E,6E)-farnesyl-diphosphate:isopentenyl-diphosphate cistransferase (adding 10--55 isopentenyl units)
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| Reaction(IUBMB) |
(2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate = n diphosphate + ditrans,polycis-polyprenyl diphosphate (n = 10--55) [RN: R05556]
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| Reaction(KEGG) |
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| Substrate |
(2E,6E)-farnesyl diphosphate [CPD: C00448];
isopentenyl diphosphate [CPD: C00129]
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| Product |
diphosphate [CPD: C00013];
ditrans,polycis-polyprenyl diphosphate [CPD: C05859]
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| Comment |
The enzyme is involved in biosynthesis of dolichol (a long-chain polyprenol) with a saturated alpha-isoprene unit, which serves as a glycosyl carrier in protein glycosylation [1]. The yeast Saccharomyces cerevisiae has two different enzymes that catalyse this reaction. Rer2p synthesizes a well-defined family of polyprenols of 13--18 isoprene residues with dominating C(80) (16 isoprene residues) extending to C(120), while Srt1p synthesizes mainly polyprenol with 22 isoprene subunits. Largest Srt1p products reach C(290) [2]. The enzyme from Arabidopsis thaliana catalyses the formation of polyprenyl diphosphates with predominant carbon number C(120) [4].
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| History |
EC 2.5.1.87 created 2010
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| Pathway |
| ec00900 | Terpenoid backbone biosynthesis |
| ec01110 | Biosynthesis of secondary metabolites |
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| Orthology |
| K11778 | ditrans,polycis-polyprenyl diphosphate synthase |
| K19177 | dehydrodolichyl diphosphate syntase complex subunit NUS1 |
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| Genes |
» show all
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| Reference |
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| Authors |
Sato M, Fujisaki S, Sato K, Nishimura Y, Nakano A |
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| Title |
Yeast Saccharomyces cerevisiae has two cis-prenyltransferases with different properties and localizations. Implication for their distinct physiological roles in dolichol synthesis. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Poznanski J, Szkopinska A |
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| Title |
Precise bacterial polyprenol length control fails in Saccharomyces cerevisiae. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Sato M, Sato K, Nishikawa S, Hirata A, Kato J, Nakano A |
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| Title |
The yeast RER2 gene, identified by endoplasmic reticulum protein localization mutations, encodes cis-prenyltransferase, a key enzyme in dolichol synthesis. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Oh SK, Han KH, Ryu SB, Kang H |
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| Title |
Molecular cloning, expression, and functional analysis of a cis-prenyltransferase from Arabidopsis thaliana. Implications in rubber biosynthesis. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Cunillera N, Arro M, Fores O, Manzano D, Ferrer A |
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| Title |
Characterization of dehydrodolichyl diphosphate synthase of Arabidopsis thaliana, a key enzyme in dolichol biosynthesis. |
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| Journal |
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| Sequence |
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| Other DBs |
| ExplorEnz - The Enzyme Database: | 2.5.1.87 |
| ExPASy - ENZYME nomenclature database: | 2.5.1.87 |
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| LinkDB |
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