KEGG ENZYME: 2.5.1.95

ENZYME: 2.5.1.95

Entry

EC 2.5.1.95 Enzyme

Name

xanthan ketal pyruvate transferase;
KPT

Class

Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)

Sysname

phosphoenolpyruvate:D-Man-beta-(1->4)-GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol 4,6-O-(1-carboxyethan-1,1-diyl)transferase

Reaction(IUBMB)

phosphoenolpyruvate + D-Man-beta-(1->4)-D-GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol = 4,6-CH3(COO-)C-D-Man-beta-(1->4)-D-GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol + phosphate [RN:R09760]

Reaction(KEGG)

R09760

Substrate

phosphoenolpyruvate [CPD:C00074];
D-Man-beta-(1->4)-D-GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol

Product

4,6-CH3(COO-)C-D-Man-beta-(1->4)-D-GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol [CPD:C19870];
phosphate [CPD:C00009]

Comment

Involved in the biosynthesis of the polysaccharide xanthan. 30-40% of the terminal mannose residues of xanthan have a 4,6-O-(1-carboxyethan-1,1-diyl) ketal group. It also acts on the 6-O-acetyl derivative of the inner mannose unit.

History

EC 2.5.1.95 created 2011, modified 2012

Reference

1 [PMID:1657892]

Authors

Marzocca MP, Harding NE, Petroni EA, Cleary JM, Ielpi L

Title

Location and cloning of the ketal pyruvate transferase gene of Xanthomonas campestris.

Journal

J Bacteriol 173:7519-24 (1991)
DOI:10.1128/JB.173.23.7519-7524.1991

Other DBs

ExplorEnz - The Enzyme Database: