KEGG   ENZYME: 2.5.1.98
Entry
EC 2.5.1.98                 Enzyme                                 

Name
Rhizobium leguminosarum exopolysaccharide glucosyl ketal-pyruvate-transferase;
PssM;
phosphoenolpyruvate:[D-GlcA-beta-(1->4)-2-O-Ac-D-GlcA-beta-(1->4)-D-Glc-beta-(1->4)-[3-O-CH3-CH2CH(OH)C(O)-D-Gal-beta-(1->4)-D-Glc-beta-(1->4)-D-Glc-beta-(1->4)-D-Glc-beta-(1->6)]-2(or 3)-O-Ac-D-Glc-alpha-(1->6)]n 4,6-O-(1-carboxyethan-1,1-diyl)transferase
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
Sysname
phosphoenolpyruvate:[beta-D-GlcA-(1->4)-2-O-Ac-beta-D-GlcA-(1->4)-beta-D-Glc-(1->4)-[3-O-CH3-CH2CH(OH)C(O)-4,6-CH3(COO-)C-beta-D-Gal-(1->4)-beta-D-Glc-(1->4)-beta-D-Glc-(1->4)-beta-D-Glc-(1->6)]-2(or 3)-O-Ac-alpha-D-Glc-(1->6)]n 4,6-O-(1-carboxyethan-1,1-diyl)transferase
Reaction(IUBMB)
phosphoenolpyruvate + [beta-D-GlcA-(1->4)-2-O-Ac-beta-D-GlcA-(1->4)-beta-D-Glc-(1->4)-[3-O-(CH3CH(OH)CH2C(O))-4,6-CH3(COO-)C-beta-D-Gal-(1->4)-beta-D-Glc-(1->4)-beta-D-Glc-(1->4)-beta-D-Glc-(1->6)]-2(or 3)-O-Ac-alpha-D-Glc-(1->6)]n = [beta-D-GlcA-(1->4)-2-O-Ac-beta-D-GlcA-(1->4)-beta-D-Glc-(1->4)-[3-O-(CH3CH(OH)CH2C(O))-4,6-CH3(COO-)C-beta-D-Gal-(1->3)-4,6-CH3(COO-)C-beta-D-Glc-(1->4)-beta-D-Glc-(1->4)-beta-D-Glc-(1->6)]-2(or 3)-O-Ac-alpha-D-Glc-(1->6)]n + phosphate
Substrate
phosphoenolpyruvate [CPD:C00074];
[beta-D-GlcA-(1->4)-2-O-Ac-beta-D-GlcA-(1->4)-beta-D-Glc-(1->4)-[3-O-(CH3CH(OH)CH2C(O))-4,6-CH3(COO-)C-beta-D-Gal-(1->4)-beta-D-Glc-(1->4)-beta-D-Glc-(1->4)-beta-D-Glc-(1->6)]-2-O-Ac-alpha-D-Glc-(1->6)]n;
[beta-D-GlcA-(1->4)-2-O-Ac-beta-D-GlcA-(1->4)-beta-D-Glc-(1->4)-[3-O-(CH3CH(OH)CH2C(O))-4,6-CH3(COO-)C-beta-D-Gal-(1->4)-beta-D-Glc-(1->4)-beta-D-Glc-(1->4)-beta-D-Glc-(1->6)]-3-O-Ac-alpha-D-Glc-(1->6)]n
Product
[beta-D-GlcA-(1->4)-2-O-Ac-beta-D-GlcA-(1->4)-beta-D-Glc-(1->4)-[3-O-(CH3CH(OH)CH2C(O))-4,6-CH3(COO-)C-beta-D-Gal-(1->3)-4,6-CH3(COO-)C-beta-D-Glc-(1->4)-beta-D-Glc-(1->4)-beta-D-Glc-(1->6)]-2-O-Ac-alpha-D-Glc-(1->6)]n;
[beta-D-GlcA-(1->4)-2-O-Ac-beta-D-GlcA-(1->4)-beta-D-Glc-(1->4)-[3-O-(CH3CH(OH)CH2C(O))-4,6-CH3(COO-)C-beta-D-Gal-(1->3)-4,6-CH3(COO-)C-beta-D-Glc-(1->4)-beta-D-Glc-(1->4)-beta-D-Glc-(1->6)]-3-O-Ac-alpha-D-Glc-(1->6)]n;
phosphate [CPD:C00009]
Comment
The enzyme is responsible for pyruvylation of the subterminal glucose in the acidic octasaccharide repeating unit of the exopolysaccharide of Rhizobium leguminosarum (bv. viciae strain VF39) which is necessary to establish nitrogen-fixing symbiosis with Pisum sativum, Vicia faba, and Vicia sativa.
History
EC 2.5.1.98 created 2012, modified 2018
Orthology
K21154  exopolysaccharide glucosyl ketal-pyruvate-transferase
Genes
RET: RHE_CH03215(pssM)
REL: REMIM1_CH03271
REI: IE4771_CH03482
RLE: RL3645(pssM)
RLG: Rleg_3215
RLB: RLEG3_26625
RLU: RLEG12_26080
RHN: AMJ98_CH03418
RHX: AMK02_CH03307
REZ: AMJ99_CH03467
RAD: CO657_15205
 » show all
Reference
1  [PMID:20233262]
  Authors
Ivashina TV, Fedorova EE, Ashina NP, Kalinchuk NA, Druzhinina TN, Shashkov AS, Shibaev VN, Ksenzenko VN
  Title
Mutation in the pssM gene encoding ketal pyruvate transferase leads to disruption of Rhizobium leguminosarum bv. viciae-Pisum sativum symbiosis.
  Journal
J Appl Microbiol 109:731-42 (2010)
DOI:10.1111/j.1365-2672.2010.04702.x
  Sequence
[rle:RL3645]
Other DBs
ExplorEnz - The Enzyme Database: 2.5.1.98
IUBMB Enzyme Nomenclature: 2.5.1.98
ExPASy - ENZYME nomenclature database: 2.5.1.98
BRENDA, the Enzyme Database: 2.5.1.98
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