Entry |
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Name |
neamine transaminase;
glutamate---6'-dehydroparomamine aminotransferase;
btrB (gene name);
neoN (gene name);
kacL (gene name)
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Class |
Transferases;
Transferring nitrogenous groups;
Transaminases
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Sysname |
neamine:2-oxoglutarate aminotransferase
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Reaction(IUBMB) |
neamine + 2-oxoglutarate = 6'-dehydroparomamine + L-glutamate [RN: R08897]
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Reaction(KEGG) |
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Substrate |
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Product |
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Comment |
The reaction occurs in vivo in the opposite direction. Involved in the biosynthetic pathways of several clinically important aminocyclitol antibiotics, including kanamycin B, butirosin, neomycin and ribostamycin. Works in combination with EC 1.1.3.43, paromamine 6-oxidase, to replace the 6'-hydroxy group of paromamine with an amino group. The enzyme from the bacterium Streptomyces kanamyceticus can also catalyse EC 2.6.1.94, 2'-deamino-2'-hydroxyneamine transaminase, which leads to production of kanamycin A [3]. The enzyme from the bacterium Streptomyces fradiae can also catalyse EC 2.6.1.95, leading to production of neomycin C [2].
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History |
EC 2.6.1.93 created 2012
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Pathway |
ec00524 | Neomycin, kanamycin and gentamicin biosynthesis |
ec01110 | Biosynthesis of secondary metabolites |
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Orthology |
K20567 | neamine transaminase / neomycin C transaminase |
K20568 | neamine transaminase / 2'-deamino-2'-hydroxyneamine transaminase |
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Genes |
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Reference |
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Authors |
Huang F, Spiteller D, Koorbanally NA, Li Y, Llewellyn NM, Spencer JB |
Title |
Elaboration of neosamine rings in the biosynthesis of neomycin and butirosin. |
Journal |
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Reference |
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Authors |
Clausnitzer D, Piepersberg W, Wehmeier UF |
Title |
The oxidoreductases LivQ and NeoQ are responsible for the different 6'-modifications in the aminoglycosides lividomycin and neomycin. |
Journal |
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Reference |
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Authors |
Park JW, Park SR, Nepal KK, Han AR, Ban YH, Yoo YJ, Kim EJ, Kim EM, Kim D, Sohng JK, Yoon YJ |
Title |
Discovery of parallel pathways of kanamycin biosynthesis allows antibiotic manipulation. |
Journal |
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Other DBs |
ExplorEnz - The Enzyme Database: | 2.6.1.93 |
ExPASy - ENZYME nomenclature database: | 2.6.1.93 |
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LinkDB |
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