| Entry |
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| Name |
DNA primase AEP;
archaeo-eukaryotic primase;
AEP;
PrimPol
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| Class |
Transferases;
Transferring phosphorus-containing groups;
Nucleotidyltransferases
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| Sysname |
(deoxy)nucleotide 5'-triphosphate:single-stranded DNA (deoxy)nucleotidyltransferase (DNA or DNA-RNA hybrid synthesizing)
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| Reaction(IUBMB) |
(1) ssDNA + n NTP = ssDNA/pppN(pN)n-1 hybrid + (n-1) diphosphate;
(2) ssDNA + n dNTP = ssDNA/pppdN(pdN)n-1 hybrid + (n-1) diphosphate
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| Substrate |
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| Product |
ssDNA/pppN(pN)n-1 hybrid;
(n-1) diphosphate;
ssDNA/pppdN(pdN)n-1 hybrid
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| Comment |
The enzyme, which is found in eukaryota and archaea, catalyses the synthesis of short RNA or DNA sequences which are used as primers for EC 2.7.7.7, DNA-directed DNA polymerase.
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| History |
EC 2.7.7.102 created 2018
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| Orthology |
| K02683 | DNA primase small subunit |
| K02684 | DNA primase small subunit |
| K22761 | DNA-directed primase/polymerase protein |
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| Genes |
» show all
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| Reference |
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| Authors |
Desogus G, Onesti S, Brick P, Rossi M, Pisani FM |
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| Title |
Identification and characterization of a DNA primase from the hyperthermophilic archaeon Methanococcus jannaschii. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Arezi B, Kuchta RD |
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| Title |
Eukaryotic DNA primase. |
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| Journal |
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| Reference |
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| Authors |
Liu L, Komori K, Ishino S, Bocquier AA, Cann IK, Kohda D, Ishino Y |
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| Title |
The archaeal DNA primase: biochemical characterization of the p41-p46 complex from Pyrococcus furiosus. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Lao-Sirieix SH, Bell SD |
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| Title |
The heterodimeric primase of the hyperthermophilic archaeon Sulfolobus solfataricus possesses DNA and RNA primase, polymerase and 3'-terminal nucleotidyl transferase activities. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Baranovskiy AG, Zhang Y, Suwa Y, Babayeva ND, Gu J, Pavlov YI, Tahirov TH |
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| Title |
Crystal structure of the human primase. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Guilliam TA, Keen BA, Brissett NC, Doherty AJ |
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| Title |
Primase-polymerases are a functionally diverse superfamily of replication and repair enzymes. |
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| Journal |
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| Other DBs |
| ExPASy - ENZYME nomenclature database: | 2.7.7.102 |
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| LinkDB |
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