Transferases;
Transferring phosphorus-containing groups;
Nucleotidyltransferases
BRITE hierarchy

(deoxy)nucleotide 5'-triphosphate:single-stranded DNA (deoxy)nucleotidyltransferase (DNA or DNA-RNA hybrid synthesizing)

(1) ssDNA + n NTP = ssDNA/pppN(pN)n-1 hybrid + (n-1) diphosphate;
(2) ssDNA + n dNTP = ssDNA/pppdN(pdN)n-1 hybrid + (n-1) diphosphate

ssDNA;
NTP [CPD:C00201];
dNTP [CPD:C06604]

The enzyme, which is found in eukaryota and archaea, catalyses the synthesis of short RNA or DNA sequences which are used as primers for EC 2.7.7.7, DNA-directed DNA polymerase.

EC 2.7.7.102 created 2018

1  [PMID:10536154]

Desogus G, Onesti S, Brick P, Rossi M, Pisani FM

Identification and characterization of a DNA primase from the hyperthermophilic archaeon Methanococcus jannaschii.

Nucleic Acids Res 27:4444-50 (1999)
DOI:10.1093/nar/27.22.4444

[mja:MJ_0839]

2  [PMID:11084371]

Arezi B, Kuchta RD

Eukaryotic DNA primase.

Trends Biochem Sci 25:572-6 (2000)
DOI:10.1016/s0968-0004(00)01680-7

3  [PMID:11584001]

Liu L, Komori K, Ishino S, Bocquier AA, Cann IK, Kohda D, Ishino Y

The archaeal DNA primase: biochemical characterization of the p41-p46 complex from Pyrococcus furiosus.

J Biol Chem 276:45484-90 (2001)
DOI:10.1074/jbc.M106391200

[pfu:PF0110 PF0111]

4  [PMID:15561142]

Lao-Sirieix SH, Bell SD

The heterodimeric primase of the hyperthermophilic archaeon Sulfolobus solfataricus possesses DNA and RNA primase, polymerase and 3'-terminal nucleotidyl transferase activities.

J Mol Biol 344:1251-63 (2004)
DOI:10.1016/j.jmb.2004.10.018

[sso:SSO1048 SSO0557]

5  [PMID:25550159]

Baranovskiy AG, Zhang Y, Suwa Y, Babayeva ND, Gu J, Pavlov YI, Tahirov TH

Crystal structure of the human primase.

J Biol Chem 290:5635-46 (2015)
DOI:10.1074/jbc.M114.624742

[hsa:5557 5558]

6  [PMID:26109351]

Guilliam TA, Keen BA, Brissett NC, Doherty AJ

Primase-polymerases are a functionally diverse superfamily of replication and repair enzymes.

Nucleic Acids Res 43:6651-64 (2015)
DOI:10.1093/nar/gkv625