Entry |
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Name |
DNA primase AEP;
archaeo-eukaryotic primase;
AEP;
PrimPol
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Class |
Transferases;
Transferring phosphorus-containing groups;
Nucleotidyltransferases
|
Sysname |
(deoxy)nucleotide 5'-triphosphate:single-stranded DNA (deoxy)nucleotidyltransferase (DNA or DNA-RNA hybrid synthesizing)
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Reaction(IUBMB) |
(1) ssDNA + n NTP = ssDNA/pppN(pN)n-1 hybrid + (n-1) diphosphate;
(2) ssDNA + n dNTP = ssDNA/pppdN(pdN)n-1 hybrid + (n-1) diphosphate
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Substrate |
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Product |
ssDNA/pppN(pN)n-1 hybrid;
(n-1) diphosphate;
ssDNA/pppdN(pdN)n-1 hybrid
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Comment |
The enzyme, which is found in eukaryota and archaea, catalyses the synthesis of short RNA or DNA sequences which are used as primers for EC 2.7.7.7, DNA-directed DNA polymerase.
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History |
EC 2.7.7.102 created 2018
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Orthology |
K02683 | DNA primase small subunit |
K02684 | DNA primase small subunit |
K22761 | DNA-directed primase/polymerase protein |
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Genes |
» show all
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Reference |
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Authors |
Desogus G, Onesti S, Brick P, Rossi M, Pisani FM |
Title |
Identification and characterization of a DNA primase from the hyperthermophilic archaeon Methanococcus jannaschii. |
Journal |
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Sequence |
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Reference |
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Authors |
Arezi B, Kuchta RD |
Title |
Eukaryotic DNA primase. |
Journal |
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Reference |
|
Authors |
Liu L, Komori K, Ishino S, Bocquier AA, Cann IK, Kohda D, Ishino Y |
Title |
The archaeal DNA primase: biochemical characterization of the p41-p46 complex from Pyrococcus furiosus. |
Journal |
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Sequence |
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Reference |
|
Authors |
Lao-Sirieix SH, Bell SD |
Title |
The heterodimeric primase of the hyperthermophilic archaeon Sulfolobus solfataricus possesses DNA and RNA primase, polymerase and 3'-terminal nucleotidyl transferase activities. |
Journal |
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Sequence |
|
Reference |
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Authors |
Baranovskiy AG, Zhang Y, Suwa Y, Babayeva ND, Gu J, Pavlov YI, Tahirov TH |
Title |
Crystal structure of the human primase. |
Journal |
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Sequence |
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Reference |
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Authors |
Guilliam TA, Keen BA, Brissett NC, Doherty AJ |
Title |
Primase-polymerases are a functionally diverse superfamily of replication and repair enzymes. |
Journal |
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Other DBs |
ExPASy - ENZYME nomenclature database: | 2.7.7.102 |
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LinkDB |
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