Hydrolases;
Acting on ester bonds;
Carboxylic-ester hydrolases
BRITE hierarchy

diacylglycerol acylhydrolase

(1) diacylglycerol + H2O = monoacylglycerol + a carboxylate [RN:R05209];
(2) triacylglycerol + H2O = diacylglycerol + a carboxylate [RN:R01369];
(3) monoacylglycerol + H2O = glycerol + a carboxylate [RN:R07293]

R01369 R05209 R07293
Reaction

diacylglycerol [CPD:C00165];
H2O [CPD:C00001];
triacylglycerol [CPD:C00422];
monoacylglycerol [CPD:C01885]

This enzyme is a serine hydrolase. Compared with other lipases, hormone-sensitive lipase has a uniquely broad substrate specificity. It hydrolyses all acylglycerols (triacylglycerol, diacylglycerol and monoacylglycerol) [2,3,4] as well as cholesteryl esters [2,4], steroid fatty acid esters [5], retinyl esters [6] and p-nitrophenyl esters [4,7]. It exhibits a preference for the 1- or 3-ester bond of its acylglycerol substrate compared with the 2-ester bond [8]. The enzyme shows little preference for the fatty acids in the triacylglycerol, although there is some increase in activity with decreasing chain length. The enzyme activity is increased in response to hormones that elevate intracellular levels of cAMP.

EC 3.1.1.79 created 2004

1  [PMID:10940339]

Holm C, Osterlund T, Laurell H, Contreras JA.

Molecular mechanisms regulating hormone-sensitive lipase and lipolysis.

Annu Rev Nutr 20:365-93 (2000)
DOI:10.1146/annurev.nutr.20.1.365

2  [PMID:7240206]

Fredrikson G, Stralfors P, Nilsson NO, Belfrage P.

Hormone-sensitive lipase of rat adipose tissue. Purification and some properties.

J Biol Chem 256:6311-20 (1981)

3  [PMID:14169138]

VAUGHAN M, BERGER JE, STEINBERG D.

HORMONE-SENSITIVE LIPASE AND MONOGLYCERIDE LIPASE  ACTIVITIES IN ADIPOSE TISSUE.

J Biol Chem 239:401-9 (1964)

4  [PMID:8912675]

Osterlund T, Danielsson B, Degerman E, Contreras JA, Edgren G, Davis RC, Schotz MC, Holm C.

Domain-structure analysis of recombinant rat hormone-sensitive lipase.

Biochem J 319 ( Pt 2):411-20 (1996)
DOI:10.1042/bj3190411

5  [PMID:3196730]

Lee FT, Adams JB, Garton AJ, Yeaman SJ.

Hormone-sensitive lipase is involved in the hydrolysis of lipoidal derivatives of estrogens and other steroid hormones.

Biochim Biophys Acta 963:258-64 (1988)
DOI:10.1016/0005-2760(88)90289-5

6  [PMID:9162045]

Wei S, Lai K, Patel S, Piantedosi R, Shen H, Colantuoni V, Kraemer FB, Blaner WS.

Retinyl ester hydrolysis and retinol efflux from BFC-1beta adipocytes.

J Biol Chem 272:14159-65 (1997)
DOI:10.1074/jbc.272.22.14159

7  [PMID:2794798]

Tsujita T, Ninomiya H, Okuda H.

p-nitrophenyl butyrate hydrolyzing activity of hormone-sensitive lipase from bovine adipose tissue.

J Lipid Res 30:997-1004 (1989)

8  [PMID:14725507]

Yeaman SJ.

Hormone-sensitive lipase--new roles for an old enzyme.

Biochem J 379:11-22 (2004)
DOI:10.1042/BJ20031811