KEGG   ENZYME: 3.1.11.8Help
Entry
EC 3.1.11.8                 Enzyme                                 

Name
guanosine-5'-diphospho-5'-[DNA] diphosphatase;
aprataxin;
pp5'G5'DNA diphosphatase;
pp5'G5'-DNA guanylate hydrolase;
APTX (gene name);
HNT3 (gene name)
Class
Hydrolases;
Acting on ester bonds;
Exodeoxyribonucleases producing 5'-phosphomonoesters
BRITE hierarchy
Sysname
guanosine-5'-diphospho-5'-[DNA] hydrolase (guanosine 5'-phosphate-forming)
Reaction(IUBMB)
guanosine-5'-diphospho-5'-[DNA] + H2O = phospho-5'-[DNA] + GMP
Substrate
guanosine-5'-diphospho-5'-[DNA];
H2O [CPD:C00001]
Product
phospho-5'-[DNA];
GMP [CPD:C00144]
Comment
Aprataxin is a DNA-binding protein that catalyses (among other activities) the 5' decapping of Gpp-DNA (formed by homologs of RtcB3 from the bacterium Myxococcus xanthus). The enzyme binds the guanylate group to a histidine residue at its active site, forming a covalent enzyme-nucleotide phosphate intermediate, followed by the hydrolysis of the guanylate from the nucleic acid and eventual release. The enzyme forms a 5'-phospho terminus that can be efficiently joined by "classical" ligases. The enzyme also possesses the activitiy of EC 3.1.11.7, adenosine-5'-diphospho-5'-[DNA] diphosphatase and EC 3.1.12.2, DNA-3'-diphospho-5'-guanosine diphosphatase.
History
EC 3.1.11.8 created 2017
Orthology
K10863  aprataxin
Genes
HSA: 54840(APTX)
PTR: 465037(APTX)
PPS: 100972572(APTX)
GGO: 101143636(APTX)
PON: 100439490(APTX)
NLE: 100592983(APTX)
MCC: 702671(APTX)
MCF: 102135987(APTX)
CSAB: 103219297(APTX)
RRO: 104674274(APTX)
RBB: 108518241(APTX)
CJC: 100410996(APTX)
SBQ: 101046890(APTX)
MMU: 66408(Aptx)
MCAL: 110292499(Aptx)
MPAH: 110338795(Aptx)
RNO: 259271(Aptx)
MUN: 110566705(Aptx)
CGE: 100774231(Aptx)
NGI: 103741597(Aptx)
HGL: 101719882(Aptx)
CCAN: 109681409(Aptx)
OCU: 100351115(APTX) 103348750
TUP: 102475725(APTX)
CFA: 442943(APTX)
VVP: 112927557(APTX)
AML: 100477925(APTX)
UMR: 103667027(APTX)
UAH: 113260533(APTX)
ORO: 101386234(APTX)
FCA: 101097680(APTX)
PTG: 102950944(APTX)
PPAD: 109252050(APTX)
AJU: 106976035(APTX)
BTA: 359714(APTX)
BOM: 102268362(APTX)
BIU: 109563163(APTX)
BBUB: 102391016(APTX)
CHX: 102186869(APTX)
OAS: 101105385(APTX)
SSC: 387596(APTX)
CFR: 102516533(APTX)
CDK: 105085570(APTX)
BACU: 102997771(APTX)
LVE: 103085186(APTX)
OOR: 101283923(APTX)
DLE: 111163193(APTX)
PCAD: 102980098(APTX)
ECB: 100068091(APTX)
EPZ: 103555028(APTX)
EAI: 106841467(APTX)
MYB: 102241189(APTX)
MYD: 102760132(APTX)
MNA: 107544998(APTX)
HAI: 109371794(APTX)
DRO: 112304535(APTX)
PALE: 102880269(APTX)
RAY: 107502755(APTX)
MJV: 108404115(APTX)
LAV: 100660757(APTX)
TMU: 101342573
MDO: 100020823(APTX)
SHR: 100934939(APTX)
PCW: 110204302 110220574(APTX)
OAA: 100075506(APTX)
GGA: 395173(APTX)
MGP: 100543078
CJO: 107306647(APTX)
NMEL: 110389533(APTX)
APLA: 101792244(APTX)
ACYG: 106040205(APTX)
TGU: 100226520(APTX)
LSR: 110474048(APTX)
SCAN: 103821836(APTX)
GFR: 102042023(APTX)
FAB: 101807349(APTX)
PHI: 102109622(APTX)
PMAJ: 107216303(APTX)
CCAE: 111941318(APTX)
CCW: 104689793(APTX)
ETL: 114057879(APTX)
FPG: 101917451(APTX)
FCH: 102057105(APTX)
CLV: 102086630(APTX)
EGZ: 104123062(APTX)
NNI: 104022636(APTX)
ACUN: 113490307(APTX)
PADL: 103921041(APTX)
AAM: 106495965(APTX)
ASN: 102386580(APTX)
AMJ: 102559093(APTX)
PSS: 102460401(APTX)
CMY: 102939347(APTX)
CPIC: 101943978(APTX)
ACS: 100558032(aptx)
PVT: 110087361(APTX)
PBI: 103066205(APTX)
PMUR: 107285612(APTX)
TSR: 106548867(APTX)
PMUA: 114587702(APTX)
GJA: 107118361(APTX)
XLA: 398657(aptx.L)
XTR: 403092(aptx)
NPR: 108798251(APTX)
DRE: 405797(aptx)
SANH: 107657991(aptx)
SGH: 107570816(aptx)
IPU: 108260610(aptx)
PHYP: 113524515(aptx)
AMEX: 103046803(aptx)
EEE: 113583868(aptx)
TRU: 445995(aptx)
LCO: 104930177(aptx)
NCC: 104954692(aptx)
MZE: 101466233(aptx)
ONL: 100708465(aptx)
OLA: 101162025(aptx)
XMA: 102232898(aptx)
XCO: 114144368(aptx)
PRET: 103465988(aptx)
CVG: 107097703(aptx)
NFU: 107375251(aptx)
KMR: 108237716(aptx)
ALIM: 106517163(aptx)
AOCE: 111579518(aptx)
CSEM: 103383834(aptx)
POV: 109637835(aptx)
LCF: 108896493(aptx)
SDU: 111231416(aptx)
SLAL: 111664047(aptx)
HCQ: 109519905(aptx)
BPEC: 110165402(aptx)
MALB: 109961360(aptx)
SASA: 100194567(aptx)
OTW: 112248313(aptx)
SALP: 111975108(aptx)
ELS: 105014574(aptx)
SFM: 108925927(aptx)
PKI: 111844213(aptx)
LCM: 102345286(APTX)
CMK: 103187207(aptx)
RTP: 109926966(aptx)
CIN: 445730(aptx)
SPU: 592796
APLC: 110981744
SKO: 102800978
DME: Dmel_CG5316(CG5316)
DSI: Dsimw501_GD19289(Dsim_GD19289)
DSR: 110188279
DPE: 6594229
MDE: 101891634
LCQ: 111683105
AAG: 5565705
AALB: 109623040
AME: 408601
BIM: 100747480
BTER: 100644640
CCAL: 108629642
OBB: 114875389
SOC: 105202804
MPHA: 105835091
AEC: 105144914
ACEP: 105620378
PBAR: 105426048
VEM: 105561077
HST: 105186905
DQU: 106741561
CFO: 105258852
LHU: 105672859
PGC: 109859789
OBO: 105275839
PCF: 106788572
NVI: 100115496
TCA: 658572
NVL: 108559963
BMOR: 101744383
PMAC: 106719089
PRAP: 111003738
HAW: 110379623
TNL: 113501361
PXY: 105386421
API: 100569003
DNX: 107166542
AGS: 114120898
RMD: 113549244
BTAB: 109035556
CLEC: 106663263
ZNE: 110834505
PVM: 113816347
CSCU: 111627534
PTEP: 107449666
PCAN: 112555301
CRG: 105322078
MYI: 110446209
OBI: 106880390
HMG: 100206030
AQU: 100640625
ATH: AT5G01310(APTX)
CRB: 17882450
BRP: 103847505
BOE: 106319492
RSZ: 108818268
THJ: 104816650
CPAP: 110809488
CIT: 102619121
TCC: 18597500
GRA: 105779441
GHI: 107899563
GAB: 108460140
EGR: 104423355
GMX: 100786762
GSJ: 114415910
VRA: 106772894
VAR: 108346640
VUN: 114167226
CCAJ: 109806112
CAM: 101510807
LJA: Lj0g3v0316429.1(Lj0g3v0316429.1)
ADU: 107475347
AIP: 107625784
LANG: 109361051
FVE: 101306149
RCN: 112192537
PPER: 18775191
PMUM: 103341550
PAVI: 110768433
MDM: 103425917
ZJU: 107430895
CSV: 101216867
CMO: 103486269
MCHA: 111015060
CMAX: 111470189
CMOS: 111452802
CPEP: 111801005
RCU: 8283140
JCU: 105648121
HBR: 110662752
MESC: 110623086
POP: 7460370
JRE: 108995190
VVI: 100259037
SLY: 101257206
SPEN: 107031205
SOT: 102578604
CANN: 107842536
NSY: 104236796
NTO: 104118789
NAU: 109219272
INI: 109181766
SIND: 105174593
HAN: 110896008
LSV: 111908638
CCAV: 112504614
DCR: 108208832
BVG: 104892527
SOE: 110791642
NNU: 104594951
OSA: 4332518
DOSA: Os03t0293400-01(Os03g0293400)
OBR: 102709720
BDI: 100839576
ATS: 109733434(LOC109733434)
SBI: 8084030
ZMA: 103634500
SITA: 101776352
PDA: 103706712
EGU: 105057122
MUS: 103974008
DCT: 110096109
PEQ: 110028380
AOF: 109842914
ATR: 18444741
PPP: 112288900
MNG: MNEG_4264
SCE: YOR258W(HNT3)
KMX: KLMA_30465(HNT3)
NCS: NCAS_0B01160(NCAS0B01160)
NDI: NDAI_0E01030(NDAI0E01030)
TBL: TBLA_0B03650(TBLA0B03650)
TDL: TDEL_0A06520(TDEL0A06520)
KAF: KAFR_0A04000(KAFR0A04000)
PIC: PICST_33883(HNT3)
CAL: CAALFM_CR03440WA(CaO19.2376) CAALFM_CR03590CA(CaO19.4383)
SLB: AWJ20_1739(HNT3)
NCR: NCU06572
NTE: NEUTE1DRAFT136002(NEUTE1DRAFT_136002)
MGR: MGG_07058
SSCK: SPSK_09595
MAW: MAC_00501
MAJ: MAA_07304
BFU: BCIN_08g06920(Bchnt3)
MBE: MBM_01023
ANI: AN2139.2
ANG: ANI_1_1686134(An15g06480)
ABE: ARB_03228
TVE: TRV_02456
PTE: PTT_12333
CNE: CNC04800
CNB: CNBC2380
ABP: AGABI1DRAFT122637(AGABI1DRAFT_122637)
ABV: AGABI2DRAFT187227(AGABI2DRAFT_187227)
MGL: MGL_0877
MRT: MRET_2631
DFA: DFA_01715
SPAR: SPRG_06657
 » show all
Taxonomy
Reference
1  [PMID:26350128]
  Authors
Maughan WP, Shuman S
  Title
Characterization of 3'-Phosphate RNA Ligase Paralogs RtcB1, RtcB2, and RtcB3 from Myxococcus xanthus Highlights DNA and RNA 5'-Phosphate Capping Activity of RtcB3.
  Journal
J Bacteriol 197:3616-24 (2015)
DOI:10.1128/JB.00631-15
Other DBs
ExplorEnz - The Enzyme Database: 3.1.11.8
IUBMB Enzyme Nomenclature: 3.1.11.8
ExPASy - ENZYME nomenclature database: 3.1.11.8
BRENDA, the Enzyme Database: 3.1.11.8
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