KEGG   ENZYME: 3.1.4.60Help
Entry
EC 3.1.4.60                 Enzyme                                 

Name
pApA phosphodiesterase;
pde2 (gene name);
pApA hydrolase
Class
Hydrolases;
Acting on ester bonds;
Phosphoric-diester hydrolases
BRITE hierarchy
Sysname
5'-O-phosphonoadenylyl-(3'->5')-adenosine phosphohydrolase
Reaction(IUBMB)
5'-O-phosphonoadenylyl-(3'->5')-adenosine + H2O = 2 AMP [RN:R12346]
Reaction(KEGG)
Substrate
5'-O-phosphonoadenylyl-(3'->5')-adenosine [CPD:C22092];
H2O [CPD:C00001]
Product
AMP [CPD:C00020]
Comment
The enzyme, characterized from the Gram-positive bacterium Staphylococcus aureus, is a cytoplasmic protein that contains a DHH/DHHA1 domain. It can act on cyclic di-3',5'-adenylate with a much lower activity (cf. EC 3.1.4.59, cyclic-di-AMP phosphodiesterase). Activity requires Mn2+ and is inhibited by ppGpp.
History
EC 3.1.4.60 created 2019
Reference
1  [PMID:24013631]
  Authors
Bai Y, Yang J, Eisele LE, Underwood AJ, Koestler BJ, Waters CM, Metzger DW, Bai G
  Title
Two DHH subfamily 1 proteins in Streptococcus pneumoniae possess cyclic di-AMP phosphodiesterase activity and affect bacterial growth and virulence.
  Journal
J Bacteriol 195:5123-32 (2013)
  Sequence
[spd:SPD_1153]
Reference
2  [PMID:24566626]
  Authors
Ye M, Zhang JJ, Fang X, Lawlis GB, Troxell B, Zhou Y, Gomelsky M, Lou Y, Yang XF
  Title
DhhP, a cyclic di-AMP phosphodiesterase of Borrelia burgdorferi, is essential for cell growth and virulence.
  Journal
Infect Immun 82:1840-9 (2014)
  Sequence
[bbu:BB_0619]
Reference
3  [PMID:26078723]
  Authors
Tang Q, Luo Y, Zheng C, Yin K, Ali MK, Li X, He J
  Title
Functional Analysis of a c-di-AMP-specific Phosphodiesterase MsPDE from Mycobacterium smegmatis.
  Journal
Int J Biol Sci 11:813-24 (2015)
  Sequence
Reference
4  [PMID:26691161]
  Authors
Kuipers K, Gallay C, Martinek V, Rohde M, Martinkova M, van der Beek SL, Jong WS, Venselaar H, Zomer A, Bootsma H, Veening JW, de Jonge MI
  Title
Highly conserved nucleotide phosphatase essential for membrane lipid homeostasis  in Streptococcus pneumoniae.
  Journal
Mol Microbiol 101:12-26 (2016)
  Sequence
[spn:SP_1298]
Reference
5  [PMID:27834680]
  Authors
Bowman L, Zeden MS, Schuster CF, Kaever V, Grundling A
  Title
New Insights into the Cyclic Di-adenosine Monophosphate (c-di-AMP) Degradation Pathway and the Requirement of the Cyclic Dinucleotide for Acid Stress Resistance in Staphylococcus aureus.
  Journal
J Biol Chem 291:26970-26986 (2016)
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.1.4.60
IUBMB Enzyme Nomenclature: 3.1.4.60
ExPASy - ENZYME nomenclature database: 3.1.4.60
BRENDA, the Enzyme Database: 3.1.4.60
LinkDB All DBs

DBGET integrated database retrieval system