EC 184.108.40.206 Enzyme
cellulose 1,4-beta-cellobiosidase (reducing end);
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
4-beta-D-glucan cellobiohydrolase (reducing end)
Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and similar substrates, releasing cellobiose from the reducing ends of the chains.
Some exocellulases, most of which belong to the glycoside hydrolase family 48 (GH48, formerly known as cellulase family L), act at the reducing ends of cellulose and similar substrates. The CelS enzyme from Clostridium thermocellum is the most abundant subunit of the cellulosome formed by the organism. It liberates cellobiose units from the reducing end by hydrolysis of the glycosidic bond, employing an inverting reaction mechanism . Different from EC
, which attacks cellulose from the non-reducing end.
EC 220.127.116.11 created 2011
Barr BK, Hsieh YL, Ganem B, Wilson DB
Identification of two functionally different classes of exocellulases.
Biochemistry 35:586-92 (1996)
Saharay M, Guo H, Smith JC
Catalytic mechanism of cellulose degradation by a cellobiohydrolase, CelS.
PLoS One 5:e12947 (2010)
ExplorEnz - The Enzyme Database:
IUBMB Enzyme Nomenclature:
ExPASy - ENZYME nomenclature database:
BRENDA, the Enzyme Database:
integrated database retrieval system