KEGG   ENZYME: 3.2.1.213
Entry
EC 3.2.1.213                Enzyme                                 

Name
galactan exo-1,6-beta-galactobiohydrolase (non-reducing end);
exo-beta-1,6-galactobiohydrolase;
1,6Gal (gene name)
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
Sysname
exo-beta-(1->6)-galactobiohydrolase (non-reducing end)
Reaction(IUBMB)
Hydrolysis of (1->6)-beta-D-galactosidic linkages in arabinogalactan proteins and (1->3):(1->6)-beta-galactans to yield (1->6)-beta-galactobiose as the final product.
Comment
The enzyme, characterized from the bacterium Bifidobacterium longum, specifically hydrolyses (1->6)-beta-galactobiose from the non-reducing terminal of (1->6)-beta-D-galactooligosaccharides with a degree of polymerization (DP) of 3 or higher, using an exo mode of action. The enzyme cannot hydrolyse alpha-L-arabinofuranosylated (1->6)-beta-galactans (as found in arabinogalactans) and does not act on (1->3)-beta-D- or (1->4)-beta-D-galactans. cf. EC 3.2.1.164, galactan endo-1,6-beta-galactosidase.
History
EC 3.2.1.213 created 2020
Orthology
K24100  galactan exo-1,6-beta-galactobiohydrolase (non-reducing end)
Genes
BLO: BL0157
BLJ: BLD_1519
BLF: BLIF_1909
BLL: BLJ_1955
BLB: BBMN68_1454
BLM: BLLJ_1841
BLK: BLNIAS_00036
BLZ: BLGT_09870
BLX: GS08_10135
Reference
1  [PMID:30564851]
  Authors
Fujita K, Sakamoto A, Kaneko S, Kotake T, Tsumuraya Y, Kitahara K
  Title
Degradative enzymes for type II arabinogalactan side chains in Bifidobacterium longum subsp. longum.
  Journal
Appl Microbiol Biotechnol 103:1299-1310 (2019)
DOI:10.1007/s00253-018-9566-4
  Sequence
[blm:BLLJ_1841]
Other DBs
ExplorEnz - The Enzyme Database: 3.2.1.213
IUBMB Enzyme Nomenclature: 3.2.1.213
ExPASy - ENZYME nomenclature database: 3.2.1.213
BRENDA, the Enzyme Database: 3.2.1.213
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