KEGG   ENZYME: 3.2.2.24
Entry
EC 3.2.2.24                 Enzyme                                 

Name
ADP-ribosyl-[dinitrogen reductase] hydrolase;
azoferredoxin glycosidase;
azoferredoxin-activating enzymes;
dinitrogenase reductase-activating glycohydrolase;
ADP-ribosyl glycohydrolase;
draG (gene name)
Class
Hydrolases;
Glycosylases;
Hydrolysing N-glycosyl compounds
Sysname
ADP-D-ribosyl-[dinitrogen reductase] ADP-ribosylhydrolase
Reaction(IUBMB)
[dinitrogen reductase]-Nomega-alpha-(ADP-D-ribosyl)-L-arginine = ADP-D-ribose + [dinitrogen reductase]-L-arginine [RN:R03446]
Reaction(KEGG)
R03446
Substrate
[dinitrogen reductase]-Nomega-alpha-(ADP-D-ribosyl)-L-arginine [CPD:C01253]
Product
ADP-D-ribose [CPD:C00301];
[dinitrogen reductase]-L-arginine [CPD:C03315]
Comment
The enzyme restores the activity of EC 1.18.6.1, nitrogenase, by catalysing the removal of ADP-ribose from an arginine residue of the dinitrogenase reductase component of nitrogenase. This activity occurs only when the nitrogenase product, ammonium, is not available. The combined activity of this enzyme and EC 2.4.2.37, NAD+-dinitrogen-reductase ADP-D-ribosyltransferase, controls the level of activity of nitrogenase.
History
EC 3.2.2.24 created 1992
Orthology
K05521  ADP-ribosyl-[dinitrogen reductase] hydrolase
Genes
ECLG: EC036_25870
ECLI: ECNIH5_12390
ECLX: LI66_12740
EXF: BFV63_12745
ECLA: ECNIH3_12445
ECLC: ECR091_12380
ELG: BH714_09105
ESH: C1N69_13155 C1N69_13170
ENF: AKI40_4965
EBG: FAI37_16205 FAI37_16215
SPE: Spro_3018
MINT: C7M51_00773(draG)
BTO: WQG_5510
BTRE: F542_16530
BTRH: F543_18240
BTRA: F544_5830
AVT: NCTC3438_00628(draG)
XCC: XCC2928(draG)
XCB: XC_1180
XPH: XppCFBP6546_03125(XppCFBP6546P_03125)
SMZ: SMD_1618
LEZ: GLE_0134(draG) GLE_4820
LEM: LEN_4583
VTA: B1483
PPF: Pput_1073
PPT: PPS_2834
PPI: YSA_07149
PPX: T1E_2461
PPUH: B479_05450
PPUN: PP4_24590
PMON: X969_03535
PMOT: X970_03510
PFL: PFL_4115
PPRC: PFLCHA0_c41750(draG)
PPRO: PPC_4216
PSOS: POS17_4215
PANR: A7J50_3075
PSIL: PMA3_11360
AGU: AS4_26610
SSE: Ssed_2268
PIN: Ping_1906
MICT: FIU95_12295(draG)
METL: U737_04955(draG)
MBUR: EQU24_17290(draG)
TIG: THII_1991
THIS: HZT40_08840(draG)
TTP: E6P07_09455(draG)
HCH: HCH_04135
HCO: LOKO_02172(draG)
TAU: Tola_0577
NZO: SAMEA4504057_1018(draG)
BCEN: DM39_5971
BCEO: I35_5716
BMUL: NP80_4977
BCED: DM42_6112
BCON: NL30_02865
BMEC: WJ16_27940
CABA: SBC2_09970
PNA: Pnap_4684
ACRA: BSY15_3254
RGE: RGE_42850(draG)
SLT: Slit_0832
DSU: Dsui_0953
DAR: Daro_1447
DEY: HYN24_06770(draG)
AZO: azo0530(draG1)
AZA: AZKH_0794(draG)
AZD: CDA09_08370(draG)
AZR: CJ010_03520(draG)
AZQ: G3580_18980(draG)
ZPA: C3497_06280(draG)
GSU: GSU2800(draG)
GSK: KN400_2737(draG)
GME: Gmet_0680(draG)
GUR: Gura_1208
GLO: Glov_0420
GBM: Gbem_2082(draG)
GEO: Geob_2589(draG)
GEM: GM21_2136
GEB: GM18_1870
PCA: Pcar_2093(draG)
PPD: Ppro_3462
DES: DSOUD_2575(draG)
DEU: DBW_0107
DAT: HRM2_09820(draG1)
DOV: DSCO28_58830(draG)
ADE: Adeh_0432
MXA: MXAN_6916
SCL: sce7519
RPA: RPA2406(draG)
RPB: RPB_3043
RPC: RPC_0877
RPD: RPD_2408
RPE: RPE_0836
RPT: Rpal_2658
MDI: METDI1913
MEX: Mext_4887
BVR: BVIR_1910
BLAG: BLTE_24180
RCP: RCAP_rcc03017(draG)
RHC: RGUI_1804
RID: RIdsm_02182(draG_1)
ROH: FIU89_07430(draG)
NAR: Saro_0259
STAX: MC45_07505
SSAN: NX02_28370
SPMI: K663_02015
SPHB: EP837_01714(draG)
RRU: Rru_A1008
RRF: F11_05195
MAG: amb1576
MGY: MGMSRv2__0347(draG)
MGRY: MSR1_18680(draG)
MAGX: XM1_3936(draG)
MAGN: WV31_08650
AZL: AZL_007740(draG)
ALI: AZOLI_0685(draG)
ABS: AZOBR_70133(draG)
AZT: TSH58p_07620(draG)
AZZ: DEW08_14750(draG)
MAGQ: MGMAQ_3113
DEX: HWD60_15135(draG)
MGM: Mmc1_1210
AFR: AFE_1525(draG)
BMEG: BG04_5220
BACY: QF06_00065
BEO: BEH_16750
BBEV: BBEV_2508
GEA: GARCT_03298(draG_1)
PPY: PPE_03341
PPO: PPM_3580(draG)
PPOL: X809_33860
PPOY: RE92_19415
PMW: B2K_33220
PSAB: PSAB_03525
PRI: PRIO_1093
CSQ: CSCA_3642
HHW: NCTC503_00977(draG)
RUM: CK1_14850
BHU: bhn_I0916
EHL: EHLA_2555
PHX: KGNDJEFE_00641(draG)
STH: STH1516
SALQ: SYNTR_1288
DAE: Dtox_3565
ADG: Adeg_1446
TTM: Tthe_0504
TSH: Tsac_2819
FMA: FMG_0339
PMIC: NW74_06915
PHAR: NCTC13077_00102(draG)
DHO: Dia5BBH33_00430(draG)
PFAC: PFJ30894_01450(draG)
MMC: Mmcs_0831
MKM: Mkms_0849
MJL: Mjls_0849
MVA: Mvan_1100
MGI: Mflv_5181
MPHL: MPHLCCUG_01133(draG_2)
MHAS: MHAS_00100(draG)
MDR: MDOR_01290(draG)
COA: DR71_1740
CAQU: CAQU_11315
NFA: NFA_17820
NFR: ERS450000_02331(draG)
NSR: NS506_03392(draG)
NAD: NCTC11293_02836(draG_2)
ROP: ROP_14100
REQ: REQ_16260
RHB: NY08_153
RFA: A3L23_03538(draG_2)
RHS: A3Q41_04688(draG_2)
RHU: A3Q40_00718(draG)
RRT: 4535765_01608(draG)
RCR: NCTC10994_01257(draG)
GBR: Gbro_0383
GOM: D7316_03805(draG)
SLD: T261_6616
SPRI: SPRI_5927
SLAU: SLA_1162
SRJ: SRO_4501
MTS: MTES_3045
CFI: Celf_1207
BLIN: BLSMQ_2440
PFRE: RM25_0978
ACIJ: JS278_02241(draG)
NDK: I601_1921(draG)
NDA: Ndas_0035
STRR: EKD16_02035(draG1)
NML: Namu_2379
GOB: Gobs_3689
MMAR: MODMU_4120
KRA: Krad_4406
PAUT: Pdca_69640
AMI: Amir_6695
SESP: BN6_80580
KAL: KALB_8444
TBW: NCTC13354_01133(draG)
BPSP: AH67_02620
LET: O77CONTIG1_01077(draG)
AMR: AM1_1924
NSH: GXM_05495
HAU: Haur_2362
ATM: ANT_00250(draG)
DFC: DFI_08650
TTR: Tter_0338
OBG: Verru16b_03212(draG_1) Verru16b_03215(draG_2)
CAA: Caka_2844
AGL: PYTT_2443
PIR: VN12_05195(draG)
RUL: UC8_28210(draG)
PLH: VT85_00140(draG_1) VT85_19405(draG_2)
GMR: GmarT_37940(draG)
GES: VT84_22525(draG_2) VT84_30585(draG_3) VT84_33880(draG_4) VT84_35985(draG_5)
PBOR: BSF38_00566(draG_1) BSF38_01261(draG_2)
AGV: OJF2_22050(draG_1) OJF2_45080(draG_2)
PCAG: NCTC12856_01522(draG)
POC: NCTC13071_00618(draG)
MBAS: ALGA_4056
FLN: FLA_1625
FJG: BB050_01814(draG)
CBAL: M667_18705
TMAR: MARIT_0876
KOS: KORDIASMS9_00948(draG)
EAO: BD94_4044
CHZ: CHSO_0581
CPRV: CYPRO_0857
MARN: LN42_04595
MMAD: MMJJ_03610(draG)
MMIL: sm9_1435
MEYE: TL18_03800
MFC: BRM9_1137
MCUB: MCBB_0812
MLA: Mlab_1306
MEMA: MMAB1_1891(draG)
MPL: Mpal_2490
NPH: NP_3878A
HUT: Huta_1731
HTI: HTIA_1743
HLA: Hlac_2793
HDF: AArcSl_1638(draG) AArcSl_2246(draG2)
SALI: L593_06395
 » show all
Reference
1  [PMID:2506427]
  Authors
Fitzmaurice WP, Saari LL, Lowery RG, Ludden PW, Roberts GP.
  Title
Genes coding for the reversible ADP-ribosylation system of dinitrogenase reductase from Rhodospirillum rubrum.
  Journal
Mol Gen Genet 218:340-7 (1989)
DOI:10.1007/BF00331287
  Sequence
[rru:Rru_A1008]
Reference
2  [PMID:19477184]
  Authors
Li XD, Huergo LF, Gasperina A, Pedrosa FO, Merrick M, Winkler FK
  Title
Crystal structure of dinitrogenase reductase-activating glycohydrolase (DraG) reveals conservation in the ADP-ribosylhydrolase fold and specific features in the ADP-ribose-binding pocket.
  Journal
J Mol Biol 390:737-46 (2009)
DOI:10.1016/j.jmb.2009.05.031
Reference
3  [PMID:19706507]
  Authors
Berthold CL, Wang H, Nordlund S, Hogbom M
  Title
Mechanism of ADP-ribosylation removal revealed by the structure and ligand complexes of the dimanganese mono-ADP-ribosylhydrolase DraG.
  Journal
Proc Natl Acad Sci U S A 106:14247-52 (2009)
DOI:10.1073/pnas.0905906106
Other DBs
ExplorEnz - The Enzyme Database: 3.2.2.24
IUBMB Enzyme Nomenclature: 3.2.2.24
ExPASy - ENZYME nomenclature database: 3.2.2.24
BRENDA, the Enzyme Database: 3.2.2.24
CAS: 125626-63-3
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