KEGG   ENZYME: 3.4.21.62
Entry
EC 3.4.21.62                Enzyme                                 
Name
subtilisin;
alcalase;
alcalase 0.6L;
alcalase 2.5L;
ALK-enzyme;
bacillopeptidase A;
bacillopeptidase B;
Bacillus subtilis alkaline proteinase bioprase;
bioprase AL 15;
bioprase APL 30;
colistinase;
(see also comments);
subtilisin J;
subtilisin S41;
subtilisin Sendai;
subtilisin GX;
subtilisin E;
subtilisin BL;
genenase I;
esperase;
maxatase;
alcalase;
thermoase PC 10;
protease XXVII;
thermoase;
superase;
subtilisin DY;
subtilopeptidase;
SP 266;
savinase 8.0L;
savinase 4.0T;
kazusase;
protease VIII;
opticlean;
Bacillus subtilis alkaline proteinase;
protin A 3L;
savinase;
savinase 16.0L;
savinase 32.0 L EX;
orientase 10B;
protease S
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases
Reaction(IUBMB)
Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyses peptide amides
Comment
Subtilisin is a serine endopeptidase, type example of peptidase family S8. It contains no cysteine residues (although these are found in homologous enzymes). Species variants include subtilisin BPN' (also subtilisin B, subtilopeptidase B, subtilopeptidase C, Nagarse, Nagarse proteinase, subtilisin Novo, bacterial proteinase Novo) and subtilisin Carlsberg (subtilisin A, subtilopeptidase A, alcalase Novo). Similar enzymes are produced by various Bacillus subtilis strains and other Bacillus species [1,3]
History
EC 3.4.21.62 created 1992 (EC 3.4.21.14 created 1961 as EC 3.4.4.16, transferred 1972 to EC 3.4.21.14, modified 1986, part incorporated 1992)
Orthology
K01342  subtilisin
Genes
VNAPN96_21170
VEJVEJY3_20426
VZIG5S32_16115
PAZOAYR47_08420
PPUUPputUW4_00217
PFZAV641_21465
PPSYAOC04_18875
PVKEPZ47_01330
PBZGN234_16695
PAGRE2H98_07725
SPSWSps_01013
PSPOPSPO_b1036
PAEWKIH87_09980
MIIBTJ40_12175
MICTFIU95_10505(isp)
MDNJT25_023420
METLU737_14160
GAIIMCC3135_18295(aprE)
TTCFOKN1_1929
HHHCLM76_16285
TOLTOL_3513
TORR615_16425
AHAAHA_3478
AHYAHML_18455
AHDAI20_01980
AHRV428_19170
AHPV429_19200
AHJV469_04105
AHHRY45_17885
ADHCK627_08380
CTIRALTA_A2222
PTERC2L65_19390
PUSCKA81_09145
CDNBN940_13916
HPSEHPF_14095
PBHAAW51_0831
JAJEKL02_11675
MASZC9I28_00125
MTIMDIR46_22305
MASYDPH57_21545
MPLIE1742_17405
DUGHH213_00655
ARESIWH25_04775
GSKKN400_2097
GEMGM21_1195
GPIGPICK_05335
GAOA2G06_06505
GBMGbem_3055
PACEA6070_09530
DMLDmul_39070
DLIdnl_31890
DMMdnm_002940
MLNA9174_32325
RJGCCGE525_36295 CCGE525_37610
RGRFZ934_20880
AOLS58_68990
BRQCIT40_26285
BGZXH91_13745 XH91_19520
BSYMCIT39_25275
MEEDA075_32105
MSCBN69_1924
MROSEHO51_15640
DSHDshi_0257
MALGMALG_02172
PHAOHF685_13840
ERKCD351_04895
ERRDVR09_15400
ERFFIU90_00740(apr1)
SHUMSTHU_11850
ALIAZOLI_p20735
DEXHWD60_18910
BSUBSU10300(aprE)
BSRI33_1162
BSLA7A1_3704
BSHBSU6051_10300(aprE)
BSYI653_05205 I653_16335
BSUTBSUB_01127(aprE)
BSULBSUA_01127(aprE)
BSUSQ433_05825
BSOBSNT_07467(aprN)
BSNBSn5_17030
BSQB657_10300(aprE)
BSXC663_1054(aprE) C663_3243(aprX)
BSPU712_05250
BSSBSUW23_05210(aprE)
BSTGYO_1328
BLIBL01111(apr)
BLDBLi01109(apr)
BLHBaLi_c12310(aprE)
BAYRBAM_010500
BAQBACAU_1010(aprE)
BYABANAU_0954(aprE)
BAMPB938_05060
BQYMUS_1081(aprE)
BAMLBAM5036_0953(aprE)
BAMARBAU_1012(aprE)
BAMNBASU_0989(aprE)
BAMBBAPNAU_2748(aprE)
BAMTAJ82_05900
BAMYV529_09920
BMPNG74_01058(apr)
BAOBAMF_1119(aprE)
BAZBAMTA208_04835(aprE)
BQLLL3_01118(aprE)
BXHBAXH7_01013(apr)
BAMIKSO_014610
BAMCU471_10430
BAMFU722_05330
BSIACWD84_16485
BAEBATR1942_02760
BVMB9C48_05260
BSONS101395_03724
BHTDIC78_04470
BMOJHC660_10860(aprE)
BTEQG4P54_05465(aprE)
BIQAN935_05370
BCXBCA_4942
BCYBcer98_3892
BTLBALH_4378
BWEBcerKBAB4_5739
BPUBPUM_0972
BPUMBW16_05260 BW16_11335
BPUSUP12_02845 UP12_05210 UP12_10600 UP12_18400
BJSMY9_1131
BIFN288_23435
GSTHW35_12820
BACWQR42_05055 QR42_10555
BACPSB24_04625
BACBOY17_07935
BACYQF06_04075
BACLBS34A_11440(aprE)
BALMBsLM_1072
BGYBGLY_1146(apr) BGLY_1970
BWHA9C19_20320
BXIBK049_03075 BK049_08985 BK049_16485
BALTCFN77_05165 CFN77_11185
BACSAUL54_05350
BSAFBSL056_05155 BSL056_11625
BITBIS30_15855
BSHILGQ02_01210
BHKB4U37_08185 B4U37_08190
BCOHBC6307_03110 BC6307_09490 BC6307_20400
BMQBMQ_pBM60006
BMHBMWSH_p211(alp)
BMEGBG04_5989
BFXBC359_19465 BC359_20835 BC359_20895 BC359_20955
PKSIE339_22580
BHABH0684(alp) BH0855
BCLABC0761(aprE)
BLEBleG1_0395 BleG1_3277
BGIBGM20_20425
BPFBpOF4_09070(subE) BpOF4_12080
BONA361_05130 A361_05175
BKOCKF48_03925
CGOTJ1899_03915 J1899_20685
GLIGLN3_08620
GTNGTNG_3101
GJFM493_16490
GSRGS3922_00340
PCALBV455_02091(aprE)
ANMGFC28_2287
ANLGFC29_3149
ANDGRQ40_04675
LSPBsph_0341
LGYT479_08835 T479_19860
LFUHR49_04065
LYGC1N55_15990
HMNHM131_05010
HLIHLI_15035
VIGBKP57_02100 BKP57_07055
FARABE41_017340 ABE41_019275
APAKAP3564_01855
BMURABE28_015170
PASABAOM_2408(aprE)
PPSRI6J18_01860 I6J18_07910 I6J18_17480
PSYHD0S48_07750
PSYOPB01_03765
POFGS400_07575
MDGK8L98_08770
AIAAWH56_020315
SHVAAT16_04835 AAT16_09650
ESIExig_1251
EANEab7_1202
EXMU719_06490
EXUESP131_08900
BBEBBR47_20780 BBR47_22370
BFMBP422_05500 BP422_06405 BP422_19810
BAGRBA6348_22325
BRWGOP56_11550
BCHSJNE38_11680
PJDPjdr2_0135
GYMGYMC10_0122
PPMPPSC2_00675(epr)
PPOPPM_0127(epr)
PPQPPSQR21_001320(aprE)
PPOYRE92_11030
PMSKNP414_07652
PMQPM3016_7185
PMWB2K_36240
PSABPSAB_00510
PDUPDUR_00585
PGMPGRAT_00580
PODPODO_00595
PAEFR50345_00610
PSTEPSTEL_00945
PAEAR70723_00565
PAEER70331_00580
PAEHH70357_00585
PAEJH70737_00655
PRIPRIO_0115 PRIO_6716(aprE)
PSONJI735_08260
PPEOABE82_00685
PNPIJ22_44650
POWIJ21_02130
PXLBS614_03610
PYGAWM70_18485
PSWUSY83_18965
PDHB9T62_32665
PKBB4V02_24585
PVOPVOR_02741
PLWD5F53_29015
PLENEIM92_04075
PPSCEHS13_14370 EHS13_32420
PLUTEI981_00565
PCHIPC41400_29430
PBKBack11_45130
PBACHUB98_17660
PRZGZH47_14975
PLYCGXP70_02000
PSOPKP014_03810
PTRIKDC22_00575
PTJJRJ22_00580
TCOTheco_3545
ASOCCB4_03352(aprE)
COHEAV92_20340
COHNKCTCHS21_04720
AFXJZ786_07280
SIVSSIL_3520
SSILSOLI23_00835
PLNPlano_0709
PRTAUC31_05580
PLLI858_003580
PANABBH88_16715
PHCBBI08_09060
PMARB0X71_14850
PPLABBI15_00140
PLXCW734_05890
PMATBBI11_05365
PDECH1Q58_04510
PGQFK545_12565
JEOJMA_06320 JMA_09790
SURESporoP32a_12710
PANCE2636_12390
PLAYDNR44_007855 DNR44_016300
NTRB0W44_11455 B0W44_14375 B0W44_14495
LFBC1X05_12925
TVUAB849_007900
KPULGXN76_12330
KEBGXN75_13860
PABSJIR001_24090
CTHCthe_3137
ANRAna3638_00755
EACEAL2_808p03370(epr)
STHSTH1033
DCADesca_0077
DFGB0537_01205
DKUDesku_1439
HMOHM1_2600
CTHMCFE_0732 CFE_0735
MTHOMOTHE_c20730(aprE)
MTHZMOTHA_c21500(aprE)
TOCToce_0485
NTHNther_2143
AARAcear_1993
KPARJL105_08405
LPILLIP_0524
RRT4535765_02483(aprE)
GBRGbro_2515
TPRTpau_1297
SBHSBI_04305
STIRDDW44_06165
LYDD7I47_12140
ARLAFL94_13945
ARUASPU41_03075
ACRYAC20117_15780
ARTPE5206_05210
AAIAARI_27140
GCRGcLGCM259_2901
GLUF0M17_13330
ICAIntca_0489
NOYEXE57_14510
TCUTcur_1456
NCXNocox_04630(apr2)
SENSACE_3537
SACGFDZ84_30800
PSEAWY02_07925
PSEQAD006_24635
PECQAD017_04225
PHHAFB00_21910
PAUTPdca_21980
KPHYAOZ06_30845
ALOCRK57421
SACEGIY23_04895
ASEACPL_4119(aprA)
ACTSACWT_3990
AIHAiant_24880
LETO77CONTIG1_01619
LBOLBWT_56880
THEUHPC62_19325
MIQB5D77_17445
CYTcce_0328
NOPNos7524_3044
NOECLI64_27730
NAZAazo_1099
ANBANA_C13330
ACYAnacy_0038
AWAAA650_04050
CSGCylst_5288
RSINB6N60_03979
CALOCal7507_1584
CALTCal6303_2875
CALHIJ00_03035 IJ00_14910
FISFIS3754_31010 FIS3754_47610
NSPBMF81_02168
DOUBMF77_00947(apr)
TOQHCG51_12215
NCNBZZ01_26765
RCARcas_2539
TROtrd_1703
DDRDeide_3p00270
DMRDeima_0142
DGODGo_PA0262
DEZDKM44_14555
DFCDFI_13995 DFI_19670
DPHEHF33_20920
TSCTSC_c03630(apr1)
THCTCCBUS3UF1_1430
TOSTheos_0005 Theos_1000
TAQTO73_2195
TBCA0O31_01516
TANTKNN15_06050
MRBMrub_0795 Mrub_1052
MREK649_03615 K649_04905
MSVMesil_3207 Mesil_3440
MTAIMtai_v1c28240
WCHwcw_1929(aprE)
ROOG5S37_11130
LUOHHL09_13970
AMUCPan181_03070
PEHSpb1_14850
PLSVT03_06235(aprN)
GESVT84_14990(apr_1) VT84_17225(aprN)
GOGC1280_02115 C1280_14680
GMSSOIL9_39940
TSPHKIH39_11465
UAMUABAM_01285 UABAM_04454
FSUFisuc_0830
FSCFSU_1274
BDOEL88_07615
PCRENCTC12858_00388
PCAGNCTC12856_01031
PMUCING2E5A_2610(aprN)
PETPEIBARAKI_4625
PSACPSM36_1910
PITPIN17_0039 PIN17_0041 PIN17_0436
FGGFSB75_01040
MRUBDEO27_008250
SBXCA265_03230
SLISlin_1710 Slin_3456
SMONAWR27_05265
SPIKEXU85_27730
STAEHNV11_15330 HNV11_19005
RUNDR864_14560
RUPDTQ70_06725 DTQ70_23935
FAEFAES_2745 FAES_3048(aprE)
FIBA6C57_18545 A6C57_23130
FLIFleli_3099
HQIH9L05_21120
FCOFCOL_07585
CLKCGC53_00640
CGNOK18_11160
CNKEG343_17540
CJGNCTC13459_00605(apr)
FTEFluta_2004
OHOOweho_0297
PAAPaes_2280
PHYAJ81_02930
MPGTheba_1167
MINFMESINF_2231
AVEArcve_0834 Arcve_0894
FPLFerp_2094
THSTES1_1967
THVADU37_CDS05690
TGGA3K92_06795
PPACPAP_01675
MBAKMSBR3_1511
MTHRMSTHT_0610
MTHEMSTHC_0110
MHORMSHOH_1113
MFZAOB57_013815
MPYMpsy_0077
MZIHWN40_07195
MHZMetho_2263
MPDMCP_1283
HARAAArcS_0205(aprE)
HAADMW046_00465 MW046_00730 MW046_00885
HALXM0R89_16480 M0R89_16485 M0R89_16490 M0R89_16495
NSALHWV07_05120 HWV07_09995
HMEHFX_5138(aprE) HFX_5181(aprE)
HBOHbor_32310 Hbor_34040 Hbor_39000
HAQDU484_07355
HAJDU500_07470
HALMFCF25_13890 FCF25_13895
HLAHlac_2196
HALNB4589_002575
HALGHUG10_06465
HALUHUG12_10560
HREK6T36_05120
HACBHbl1158_11245
HDFAArcSl_0415(aprE)
HAHHalar_2141 Halar_2573
HTUHtur_0058
HDABB347_14195
HJTDVR14_09920
HSALJMJ58_09075
HAKZJ0X25_06810
NMGNmag_1874
HXAHalxa_1240
NPENatpe_0106
NVRFEJ81_00555
NAYHYG81_10125
NGENatgr_3003 Natgr_3703
HRUHalru_0481 Halru_0482
NOUNatoc_2188
HLRHALLA_03780
HLCCHINAEXTREME11860
PFMPyrfu_1125
PDLPyrde_0048
THBN186_08410
TCBTCARB_0761
THFMA03_03015
NIWNisw_02775
TAANMY3_00666 NMY3_03174
NFNNFRAN_0204
NIUDSQ19_01525 DSQ19_03065
 » show all
Reference
1
  Authors
Ottesen, M. and Svendsen, I.
  Title
The subtilisins.
  Journal
Methods Enzymol 19:199-215 (1970)
Reference
2
  Authors
Markland, F.S. and Smith, E.L.
  Title
Subtilisins: primary structure, chemical and physical properties.
  Journal
In: Boyer, P.D. (Ed.), The Enzymes, 3rd ed., vol. 3, Academic Press, New York, 1971, p. 561-608.
Reference
3  [PMID:6221910]
  Authors
Tang H.
  Title
[The use of single-dose total body irradiation before bone marrow transplantation in treatment of leukemia]
  Journal
Zhonghua Fang She Xue Za Zhi 16:296-300 (1982)
Reference
4  [PMID:3927935]
  Authors
Nedkov P, Oberthur W, Braunitzer G.
  Title
Determination of the complete amino-acid sequence of subtilisin DY and its comparison with the primary structures of the subtilisins BPN', Carlsberg and amylosacchariticus.
  Journal
Biol Chem Hoppe Seyler 366:421-30 (1985)
DOI:10.1515/bchm3.1985.366.1.421
Reference
5  [PMID:3108260]
  Authors
Ikemura H, Takagi H, Inouye M
  Title
Requirement of pro-sequence for the production of active subtilisin E in Escherichia coli.
  Journal
J Biol Chem 262:7859-64 (1987)
  Sequence
[bsu:BSU10300]
Reference
6
  Authors
Polgar, L.
  Title
Structure and function of serine proteases.
  Journal
In: Neuberger, A. and Brocklehurst, K. (Eds.), New Comprehensive Biochemistry: Hydrolytic Enzymes, vol. 16, Elsevier, Amsterdam, 1987, p. 159-200.
Other DBs
ExplorEnz - The Enzyme Database: 3.4.21.62
IUBMB Enzyme Nomenclature: 3.4.21.62
ExPASy - ENZYME nomenclature database: 3.4.21.62
BRENDA, the Enzyme Database: 3.4.21.62
CAS: 9014-01-1
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