KEGG ENZYME: 3.5.2.1

ENZYME: 3.5.2.1

Entry

EC 3.5.2.1                  Enzyme

Name

barbiturase

Class

Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In cyclic amides

Sysname

barbiturate amidohydrolase (3-oxo-3-ureidopropanoate-forming)

Reaction(IUBMB)

barbiturate + H2O = 3-oxo-3-ureidopropanoate [RN:R02139]

Reaction(KEGG)

R02139;
(other) R03870 R10777

Substrate

barbiturate [CPD:C00813];
H2O [CPD:C00001]

Product

3-oxo-3-ureidopropanoate [CPD:C15607]

Comment

Contains zinc and is specific for barbiturate as substrate [3]. Forms part of the oxidative pyrimidine-degrading pathway in some microorganisms, along with EC 1.17.99.4 (uracil/thymine dehydrogenase) and EC 3.5.1.95 (N-malonylurea hydrolase). It was previously thought that the end-products of the reaction were malonate and urea but this has since been disproved [2]. May be involved in the regulation of pyrimidine metabolism, along with EC 2.4.2.9, uracil phosphoribosyltransferase.

History

EC 3.5.2.1 created 1961, modified 2006

Pathway

ec00240

Pyrimidine metabolism

ec01100

Metabolic pathways

Orthology

K19794

barbiturase

Genes

PSTG:

E8M01_13880

ERZ:	ER308_05780

RHA:	RHA1_ro05296

RER:	RER_58820(bar)

REY:

O5Y_28310

REB:	XU06_28640

RQI:	C1M55_30125

ROP:	ROP_27990(bar)

ROA:	Pd630_LPD01826

RFA:	A3L23_03491(bar)

RHS:	A3Q41_04764(bar)

RHOD:

AOT96_09775

RKO:	JWS14_16515

RPSK:

JWS13_28125

ICA:	Intca_1068

PEI:	H9L10_01950

TED:	U5C87_10645

SERW:

FY030_02810

ORN:	DV701_12635

BRI:	FDF13_14170

BLUT:

EW640_05135

MIK:	FOE78_19255

NCA:	Noca_1505 Noca_4270

NDK:	I601_1320(bar)

NOY:	EXE57_06140

NANO:

G5V58_15295

NMES:

H9L09_19375

NRO:	K8W59_11195

NOQ:	LN652_19335

NCQ:	K6T13_05240

NPC:	KUV85_11075

NPS:	KRR39_11145

NMAR:

HPC71_01345

PSIM:

KR76_07390

MUZ:	H4N58_05180

SGRG:

L0C25_03285

STRR:

EKD16_19160(bar)

SAIU:

J4H86_08125

AGRA:

AGRA3207_007139

AMAZ:

LUW76_42355

ACIR:

OG979_04025

SPIQ:

OHA34_36165

ACTQ:

OG417_20290

SRO:

Sros_6541

NOA:	BKM31_01725

NOW:	GBF35_36215

NCX:	Nocox_29475(bar1)

NGN:	LCN96_40605

NFS:	OIE67_08455

TBI:

Tbis_2213

MHAI:

OHB01_37300

NML:

Namu_5328

GOB:

Gobs_0181

BSD:	BLASA_0154(bar) BLASA_4559

BLAP:

MVA48_15540

MMAR:

MODMU_0176(bar)

SVI:	Svir_20970

SACC:

EYD13_13280(bar2)

PSEH:

XF36_23335

PAUT:

Pdca_02970

CAI:

Caci_1879

EKE:	EK0264_00860

JTL:	M6D93_08835

MICA:

P0L94_11675

» show all

Reference

1 [PMID:12981104]

Authors

HAYAISHI O, KORNBERG A.

Title

Metabolism of cytosine, thymine, uracil, and barbituric acid by bacterial enzymes.

Journal

J Biol Chem 197:717-32 (1952)

Reference

2 [PMID:11485332]

Authors

Soong CL, Ogawa J, Shimizu S.

Title

Novel amidohydrolytic reactions in oxidative pyrimidine metabolism: analysis of the barbiturase reaction and discovery of a novel enzyme, ureidomalonase.

Journal

Biochem Biophys Res Commun 286:222-6 (2001)
DOI:10.1006/bbrc.2001.5356

Sequence

[ag:CAC86669]

Reference

3 [PMID:11748240]

Authors

Soong CL, Ogawa J, Sakuradani E, Shimizu S

Title

Barbiturase, a novel zinc-containing amidohydrolase involved in oxidative pyrimidine metabolism.

Journal

J Biol Chem 277:7051-8 (2002)
DOI:10.1074/jbc.M110784200