KEGG ENZYME: 4.1.1.77

ENZYME: 4.1.1.77

Entry

EC 4.1.1.77                 Enzyme

Name

2-oxo-3-hexenedioate decarboxylase;
4-oxalocrotonate carboxy-lyase (misleading);
4-oxalocrotonate decarboxylase (misleading);
cnbF (gene name);
praD (gene name);
amnE (gene name);
nbaG (gene name);
xylI (gene name)

Class

Lyases;
Carbon-carbon lyases;
Carboxy-lyases

Sysname

(3E)-2-oxohex-3-enedioate carboxy-lyase (2-oxopent-4-enoate-forming)

Reaction(IUBMB)

(3E)-2-oxohex-3-enedioate = 2-oxopent-4-enoate + CO2 [RN:R02602]

Reaction(KEGG)

R02602;
(other) R05374

Substrate

(3E)-2-oxohex-3-enedioate

Product

2-oxopent-4-enoate [CPD:C00596];
CO2 [CPD:C00011]

Comment

Involved in the meta-cleavage pathway for the degradation of phenols, modified phenols and catechols. The enzyme has been reported to accept multiple tautomeric forms [1-4]. However, careful analysis of the stability of the different tautomers, as well as characterization of the enzyme that produces its substrate, EC 5.3.2.6, 2-hydroxymuconate tautomerase, showed that the actual substrate for the enzyme is (3E)-2-oxohex-3-enedioate [4].

History

EC 4.1.1.77 created 1999, modified 2011, modified 2012

Pathway

ec00362

Benzoate degradation

ec00621

Dioxin degradation

ec00622

Xylene degradation

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K01617

2-oxo-3-hexenedioate decarboxylase

Genes

PGE:	LG71_12235

SLQ:	M495_05200

PSD:

DSC_09165

RTH:	LRK53_10280(dmpH)

RBD:

ALSL_0876

PPSE:

BN5_3617(nahK)

PFUW:

KF707C_15590(bzaI) KF707C_16040(SalI)

PCQ:	PcP3B5_23510(amnE)

PNT:	G5B91_07440(dmpH)

PPW:	PputW619_2010

PPI:	ND083(nahK)

PPJ:	RK21_04234

PVR:	PverR02_14695

PKC:	PKB_3258(amne1) PKB_3606(amne3)

PALK:

PSAKL28_05950

PSOS:

POS17_2260

PFK:	PFAS1_28760

PSET:

THL1_489

PALL:

UYA_06735 UYA_06965 UYA_20140

PSEP:

C4K39_5744

PPII:

QL104_12010

PTRT:

HU722_0015015

PTAE:

NCTC10697_02308(amnE)

PHF:	NLY38_02340

PPHN:

HU825_04975(dmpH)

PSOA:

PSm6_34030

PKJ:	Q1W70_06555

PSC:	A458_06505

PSTU:

UIB01_22755

PBM:	CL52_14725

AVN:	Avin_08680(xylI) Avin_30600(lapH)

AVL:	AvCA_08680(xylI) AvCA_30600(lapH)

AVD:	AvCA6_08680(xylI) AvCA6_30600(lapH)

ACX:	Achr_32760(xylI)

HPEG:

EAO82_00540(dmpH) EAO82_09440(dmpH)

HAES:

LO767_17685(dmpH)

PPHA:

BVH74_06170

MAD:	HP15_1116 HP15_4037

MSR:	AU15_09345 AU15_21065 AU15_22100

MLQ:	ASQ50_09200

MSQ:	BKP64_13835

MARJ:

MARI_05470(tesE)

MALL:

PBN92_01685(dmpH)

MSHE:

MAALD49_03420 MAALD49_03890

MANP:

EHN06_02460(dmpH)

SWD:

Swoo_1406

SEUR:

FM038_016465

LAL:	AT746_01265

SALH:

HMF8227_02737(hpaH)

KIM:	G3T16_05370

AFUS:

EYZ66_02600(dmpH)

CYQ:

Q91_0511

CZA:	CYCME_2100

CYY:	CPC19_10500(dmpH)

BLEP:

AL038_01285(dmpH)

THIS:

HZT40_10620(dmpH)

TBN:

TBH_C0931

HCS:	FF32_08365

HBP:	HPTD01_721

HALK:

CUU95_03815

HVN:	EI420_02115

HTX:	EKK97_09975

APAC:

S7S_07005

MGEO:

CFI10_00440(dmpH) CFI10_04975(dmpH)

MSEC:

LN244_11315(dmpH)

NCU:	F0U83_03060(dmpH)

NJP:	NEJAP_0674

NIK:	F5I99_10090(dmpH) F5I99_10505(dmpH)

AJP:	AMJAP_2996

ASIM:

FE240_03475(dmpH)

ZDF:	AN401_14610

SINI:

GT972_13735(dmpH)

GBI:	PG2T_09680

NZO:	SAMEA4504057_0445(mhpD)

NCI:	NCTC10296_00916(xylJ_2)

NWD:	H3L96_04770(dmpH)

NDU:	LVJ88_00585(dmpH)

VFF:	VITFI_CDS1371

VMS:	LVJ82_11890

PTES:

JQU52_04855(dmpH)

USU:	LVJ78_03655(dmpH)

CHRO:

CXB49_03845(dmpH)

CRZ:	D1345_11510

CHAE:

CH06BL_23590

AQS:	DKK66_07205

AQL:	BXU06_16890

RSO:

RSp0892

RSE:

F504_4325

RPU:	CDC45_22075

RNC:	GO999_18490

RSM:	CMR15_mp10876

RPI:

Rpic_4619

RPF:	Rpic12D_3543

RPJ:	N234_08155 N234_12210

RIN:	ACS15_4682(amnE)

REH:	H16_B0549(h16_B0549)

CNC:	CNE_2c04950(dmpH) CNE_BB1p11980(xylI) CNE_BB1p13270(dmpH)

REU:	Reut_B5500 Reut_B5692

RME:	Rmet_1318(dmpH) Rmet_5210(amnE)

CBW:	RR42_m1052 RR42_s2483

CCUP:

BKK81_23320 BKK81_27095

CUP:	BKK80_29225 BKK80_32995

CUU:	BKK79_23880 BKK79_35175

COX:	E0W60_03750 E0W60_07780(dmpH)

CCAX:

KZ686_10010(dmpH)

CUK:	KB879_20195(dmpH)

CNAN:

A2G96_22775

BVI:	Bcep1808_7606

BUR:	Bcep18194_B2958 Bcep18194_C7643

BCJ:

BCAM2123

BCEN:

DM39_4831(dmpH)

BCEO:

I35_6005

BMUL:

NP80_3871(amnE)

BCT:

GEM_5400

BCED:

DM42_4990(dmpH)

BCON:

NL30_24585

BDF:	WI26_16000

BTEI:

WS51_00575

BPSL:

WS57_12150

BSTL:

BBJ41_10525 BBJ41_33955

BANN:

JFN94_19140(dmpH)

BARI:

NLX30_35150

BAEN:

L3V59_30410(dmpH)

BUG:	BC1001_0834

BUQ:	AC233_11180

BXE:	Bxe_A1150(amnE)

BXB:	DR64_3309(amnE)

BPX:	BUPH_05359

BPY:	Bphyt_1469

BFN:	OI25_254(amnE)

PARB:

CJU94_37885(dmpH) CJU94_38130(dmpH)

PTER:

C2L65_38850

PBRY:

NDK50_06185

PPAI:

E1956_27315

BRH:	RBRH_00201

PPK:	U875_01885 U875_18215

PPNO:

DA70_10725

PPNM:

LV28_20330

PRB:	X636_07585 X636_15900

PPUL:

RO07_14670

PSPU:

NA29_22520

PAPI:

SG18_14500

PVE:	UC34_05805

POX:	MB84_10225

PFG:	AB870_16830

PNR:	AT302_01280

PAND:

DRB87_05210

PFIB:

PI93_022455

PCOM:

NTU39_06715

CABA:

SBC2_61030(amnE)

LIMN:

HKT17_03835(dmpH)

BHZ:	ACR54_00827(amnE)

BOJ:	CBF45_01485 CBF45_04495 CBF45_06950 CBF45_16995

APES:

FOC84_00280 FOC84_00315(dmpH)

PUD:	G9Q38_15000

AKA:	TKWG_04045

AMIM:

MIM_c06230

CDN:	BN940_01171 BN940_04311

PIG:	EGT29_19205

KGY:	EHF36_01670(dmpH) EHF36_04880(dmpH) EHF36_16935

POL:	Bpro_1513 Bpro_5137

AJS:

Ajs_0225

ACID:

CBP33_01120

ACIP:

CBP36_01215

ACIN:

CBP34_01125

ACIS:

CBP35_17735

AMON:

H9L24_14170(dmpH)

VEI:

Veis_2784

VPD:	VAPA_1c22830

VBO:	CKY39_28490(dmpH)

CTT:	CtCNB1_3146

COF:	FOZ74_08325(dmpH)

CRJ:	QMY55_24790

ADN:	Alide_0340

ADK:	Alide2_0285

OTD:	J1M35_00030(dmpH)

HYL:	LPB072_18185

HPSE:

HPF_01820(tesE2) HPF_01865(tesE3)

HYN:	F9K07_02755(dmpH)

HTN:	KI616_01940 KI616_01960(dmpH)

DIH:	G7047_01665(dmpH) G7047_06945

DRG:	H9K76_11650(dmpH) H9K76_22950

SIMP:

C6571_13630(dmpH)

MELA:

C6568_07845(dmpH)

MJE:	LVC68_01035(dmpH)

STHM:

IS481_10535(dmpH)

SAQA:

OMP39_09115(dmpH)

MPT:	Mpe_A2272 Mpe_A3324

METP:

C1M51_06455(dmpH) C1M51_15395(dmpH)

RDP:	RD2015_407

MIU:	ABE85_21105

SNN:	EWH46_19320(dmpH)

IDC:	LRM40_19575(dmpH)

LCH:

Lcho_3342

JAG:	GJA_235(dmpH)

JAJ:	EKL02_01820(dmpH) EKL02_15560

HEE:	hmeg3_19570

TMJ:	P0M04_30670(dmpH)

RBU:	PG1C_09715 PG1C_14085

AIQ:	Azoinq_09670

DAR:	Daro_2773 Daro_3781

DEY:	HYN24_14125(dmpH) HYN24_14195(dmpH)

DDEN:

KI615_16865(dmpH)

FLE:	KI610_08580(dmpH) KI610_13440(dmpH) KI610_15445(dmpH)

AZO:	azo1854(lapH) azo2430(nahK)

AOA:	dqs_2007 dqs_2574

AZD:	CDA09_10340(dmpH) CDA09_10445(dmpH)

AZR:	CJ010_12255(dmpH) CJ010_15200(dmpH)

TMZ:	Tmz1t_3090

THU:	AC731_001825

THAU:

C4PIVTH_2810(dmpH)

THK:	CCZ27_10765(dmpH) CCZ27_16280(dmpH)

AZQ:	G3580_00540(dmpH) G3580_08285(dmpH)

RLE:

pRL120704

ARA:

Arad_7266

BIC:	LMTR13_06470

MRD:	Mrad2831_4067

MET:

M446_1050

MSL:

Msil_1480

MTUN:

MTUNDRAET4_3891(amnE)

DSAL:

K1X15_13430

MAAD:

AZF01_08270 AZF01_21095

MLUT:

JET14_11070

ACUT:

MRB58_02670

OCT:	FTO60_10555

CEH:	CEW89_11450

SUAM:

BOO69_22210

TEC:	AKL02_008260(dmpH)

NAR:

Saro_3851

NPP:	PP1Y_AT15722 PP1Y_AT31571

NPN:	JI59_24645

NOG:	GKE62_05080

NHUM:

PQ457_19220

SMAG:

AN936_00740

SMAZ:

LH19_00750

STER:

AOA14_03895

SGI:	SGRAN_3551(amnE)

SPHO:

C3E99_12570

SWI:	Swit_0912 Swit_3521

SPHD:

HY78_06675 HY78_22125

SPKC:

KC8_11575

SJP:	SJA_C1-11880(hpaH_xylI)

SINB:

SIDU_08325

SYB:	TZ53_09015 TZ53_23460

SHYD:

CJD35_06495(dmpH)

SUFL:

FIL70_19000

SBAR:

H5V43_20650 H5V43_22345

SPPH:

KFK14_16525

SPHY:

CHN51_06980

SPHJ:

BSL82_10450

CNA:	AB433_18115

ERY:	CP97_03155 CP97_14235

KSC:	CD178_01495(amnE)

NCB:	C0V82_23405

MAGQ:

MGMAQ_0652

GSO:	PH603_12390

HTL:

HPTL_0045

ACIB:

ACBT_0258(dmpH)

AFC:	AFAEC_1843(dmpH)

AAQI:

AAQM_0983(dmpH)

ACLO:

ACLO_a0138(dmpH)

ADZ:	ADFLV_2550(dmpH)

ARC:

ABLL_2441

AROS:

NJU99_01655(dmpH)

ALK:	ALEK_0384(dmpH)

AANA:

AANAER_1261(dmpH)

MXA:

MXAN_0925

MSD:	MYSTI_00945

MYM:	A176_006054

MFB:	MFUL124B02_05300

CCX:	COCOR_00872(dmpH)

MFU:	LILAB_04080

MMAS:

MYMAC_000948

SUR:	STAUR_2722

AGE:	AA314_01330

AVM:	JQX13_18930

PCAY:

FRD00_17510

BBAE:

FRD01_19450

BDQ:	CIK05_11235

BLI:

BL03907

BLD:	BLi03993(xylI)

BLH:	BaLi_c40070

BSON:

S101395_00392(dmpH)

BCA:

BCE_2158

BCX:

BCA_2156

BCF:

bcf_10220

BCER:

BCK_24215

BCEF:

BcrFT9_01690

BTL:

BALH_1845

BMYC:

DJ92_4639

BTRO:

FJR70_09215

BNT:	GSN03_10155

BSM:	BSM4216_0950

BGY:

BGLY_4397

BFD:	NCTC4823_00794(xylJ)

BACI:

B1NLA3E_08440 B1NLA3E_09300

GST:	HW35_13045

BACO:

OXB_2505

BACQ:

DOE78_09235

BAQU:

K6959_02310

BHK:	B4U37_13930

BMQ:

BMQ_3586

BMD:

BMD_3571

BMEG:

BG04_460

BPF:	BpOF4_12615

BDA:	FSZ17_11250

OCN:	CUC15_03685

GTN:	GTNG_3150(nbaG)

GYC:	GYMC61_2299

GYA:	GYMC52_1427

GCT:	GC56T3_2054

GMC:	GY4MC1_1597

GGH:	GHH_c14850

GJF:	M493_17910

GEJ:	A0V43_04660

GZA:	IC807_03370

GTH:	Geoth_1680

PTL:	AOT13_14435

PCAL:

BV455_00226(tesE_1)

PARG:

PspKH34_14930 PspKH34_15490

ASED:

IRT44_17615

LGY:	T479_19640

LYB:	C3943_22670

LYZ:	DCE79_01760

LPAK:

GDS87_20225

LYC:	FH508_0003705

LLB:	R6U77_10600

VIG:	BKP57_09375

VNT:	OLD84_14650

VIK:	KFZ58_14495

LAO:	AOX59_17255

FPN:	ABE65_004750

FAR:	ABE41_004890

APAK:

AP3564_06555

ACOP:

RI196_01650

BSJ:	UP17_10530

PASA:

BAOM_0499

PFRI:

L8956_11395

RUE:	DT065_12670 DT065_13795 DT065_15120 DT065_15210

SALE:

EPH95_15045

NTM:	BTDUT50_14545

NDT:	L1999_11900 L1999_17840

NCM:	QNK12_05495

NNV:	QNH39_12250 QNH39_24865

AIA:	AWH56_018055

BOU:	I5818_04805

GAJ:	MY490_08575

HYI:	K2M58_09700 K2M58_09725

BBE:	BBR47_29790

BFM:	BP422_09725

BCHS:

JNE38_24125

BHUI:

LOK74_00615

BAYD:

BSPP4475_07470(dmpH)

BBOR:

RFB14_22150

PNP:	IJ22_00700 IJ22_32390 IJ22_48600

ANX:	ACH33_11700

ASOC:

CB4_02453(amnF)

ATHE:

K3F53_07240

COH:	EAV92_02610

AAC:

Aaci_1618

AAD:

TC41_1514

AACO:

K1I37_06165

ADAU:

NZD86_00710 NZD86_06140

AFAS:

NZD89_10115

BTS:

Btus_0970

KYR:	CVV65_10340 CVV65_11285

EFF:	skT53_33780

SOB:	CSE16_14625

SPAE:

E2C16_04465 E2C16_12275

SPOO:

J3U78_11170

SURL:

BI350_00150

AUI:	APT62_01025

AVS:	AWM76_09545

CARN:

FPV25_04350

SAY:

TPY_0643

SAP:	Sulac_2996

MIT:

OCO_14790

MIA:

OCU_15230

MMAN:

MMAN_42240

MJL:

Mjls_4208

MYV:	G155_08390 G155_08470

MFT:	XA26_16790 XA26_16950

MLL:	B1R94_25050

MHEV:

MHEL_01620

MMAT:

MMAGJ_45260 MMAGJ_45420

MMON:

EWR22_21590

MGO:	AFA91_00030

MHOL:

K3U96_19110

CHN:	A605_04755

CEE:	CENDO_03490(amnE)

COK:	COCCU_04385(amnE)

NFA:

NFA_30430

NFR:	ERS450000_00296(xylJ_1) ERS450000_01021(xylJ_2)

NNO:	NONO_c62050

NTP:	CRH09_30230

NWL:	NWFMUON74_07310

RHA:	RHA1_ro03880

RER:	RER_04710 RER_59340

REY:

O5Y_02265

REB:	XU06_02465

RQI:	C1M55_02650

ROP:	ROP_37490 ROP_54170

RPY:	Y013_12560

RBY:	CEJ39_00065

RAV:	AAT18_08105

RRZ:	CS378_23120

RHQ:	IM25_04200

RHOD:

AOT96_13015

RRT:	4535765_01257(amnE)

RKO:	JWS14_41315

RGO:	KYT97_21980

ROZ:	CBI38_22780

RPSK:

JWS13_12560 JWS13_18955

REQ:	REQ_05050(amnD)

PDEF:

P9209_00360

GBR:

Gbro_0225

GTA:	BCM27_21915

GOC:	CXX93_11295

GOI:	LK459_10160

GHN:	MVF96_20965

GSI:	P5P27_10835

DIZ:	CT688_01890

TOY:	FO059_00305

SANL:

KZO11_33900

SCB:	SCAB_15181

SSX:	SACTE_4113

SRC:	M271_50380

SBH:

SBI_01536

STRT:

A8713_24465

SVU:	B1H20_29090

SGE:	DWG14_08258(amnE)

SNK:	CP967_06830

SSUB:

CP968_28065

SPLA:

CP981_35170

SCAL:

I6J39_28940

SROI:

IAG44_01280

STRY:

EQG64_09025

SGOB:

test1122_23000

SDRZ:

NEH16_30290

STUD:

STRTU_001643 STRTU_001677

SANT:

QR300_10270

KIT:	CFP65_1237

YIA:	LO772_34585

MVD:	AWU67_16690

AGY:	ATC03_02580

AMAU:

DSM26151_04170(amnE)

ASOI:

MTP13_01920

PSAI:

C3B54_11904

SALD:

FVA74_00730

LTR:	EVS81_03255

HEA:	HL652_17940

ARZ:	AUT26_08860

ACIT:

HPK19_02435

ICA:	Intca_1093

JTE:	ASJ30_00110

JLI:	EXU32_15355

JME:	EEW87_015420

JAY:	H7A72_00110

JCR:	O9K63_12595

PEI:	H9L10_13720

OCP:	NF557_14855 NF557_15600

AUS:	IPK37_11690

RAIN:

Rai3103_12620

NCA:

Noca_2055

NPI:	G7071_16490

TCU:

Tcur_0537

ACTW:

F7P10_40030

AGRA:

AGRA3207_000779 AGRA3207_003642

AMAZ:

LUW76_22725 LUW76_47885

ACIR:

OG979_20775 OG979_41955

SPIQ:

OHA34_00545

SRO:

Sros_9112

NGN:	LCN96_28045

MHAI:

OHB01_10135

FRI:	FraEuI1c_1245

NML:

Namu_0794

AMD:

AMED_8622

AMN:

RAM_44250

AMM:

AMES_8491

AMZ:

B737_8492

AOI:

AORI_7401

AJA:	AJAP_01985

AMQ:	AMETH_5437

AMYC:

CU254_17665 CU254_35760

AMYB:

BKN51_30410

AORI:

SD37_03455

AACD:

LWP59_38180

APRT:

MUY14_40600

AROO:

NQK81_00865 NQK81_28575

ATHM:

L1857_30175

ARHD:

VSH64_19090 VSH64_30305 VSH64_35370

PDX:	Psed_2523 Psed_4292

PBRO:

HOP40_26620 HOP40_31750

PPEL:

H6H00_29790

KUT:	JJ691_102210 JJ691_11960

ALO:

CRK56594

PMAD:

BAY61_16755

ACTY:

OG774_08010 OG774_09250

STP:	Strop_3524

SAQ:

Sare_3899

MAU:	Micau_4937

MIL:

ML5_3358

MICB:

MicB006_3969

MTUA:

CSH63_33405

MTEM:

GCE86_19380

MCAB:

HXZ27_25040

VMA:	VAB18032_17780 VAB18032_28741

MCRA:

ID554_21670 ID554_30890

VER:	HUT12_13555 HUT12_26050

MENO:

Jiend_41040

MCHL:

PVK74_15030

MFEU:

H1D33_00380

MPRN:

Q3V37_13160

MHAW:

RMN56_18005

PFLA:

Pflav_027520

PSUU:

Psuf_068140

PRY:	Prubr_10590 Prubr_59260

CATI:

CS0771_01260

DFU:	Dfulv_27715

NHY:	JQS43_06620

PVN:	A7sIIA15_04525

JCY:	M6B22_02780

BSOL:

FSW04_07220

IIS:

EYM_02850

» show all

Reference

1 [PMID:1732207]

Authors

Shingler V, Powlowski J, Marklund U

Title

Nucleotide sequence and functional analysis of the complete phenol/3,4-dimethylphenol catabolic pathway of Pseudomonas sp. strain CF600.

Journal

J Bacteriol 174:711-24 (1992)
DOI:10.1128/JB.174.3.711-724.1992

Sequence

[ag:CAA43228]

Reference

2 [PMID:9683650]

Authors

Takenaka S, Murakami S, Shinke R, Aoki K

Title

Metabolism of 2-aminophenol by Pseudomonas sp. AP-3: modified meta-cleavage pathway.

Journal

Arch Microbiol 170:132-7 (1998)
DOI:10.1007/s002030050624

Reference

3 [PMID:10651637]

Authors

Stanley TM, Johnson WH Jr, Burks EA, Whitman CP, Hwang CC, Cook PF

Title

Expression and stereochemical and isotope effect studies of active 4-oxalocrotonate decarboxylase.

Journal

Biochemistry 39:718-26 (2000)
DOI:10.1021/bi9918902

Reference

4 [PMID:17902707]

Authors

Wang SC, Johnson WH Jr, Czerwinski RM, Stamps SL, Whitman CP

Title

Kinetic and stereochemical analysis of YwhB, a 4-oxalocrotonate tautomerase homologue in Bacillus subtilis: mechanistic implications for the YwhB- and 4-oxalocrotonate tautomerase-catalyzed reactions.

Journal

Biochemistry 46:11919-29 (2007)
DOI:10.1021/bi701231a

Reference

5 [PMID:19717587]

Authors

Kasai D, Fujinami T, Abe T, Mase K, Katayama Y, Fukuda M, Masai E

Title

Uncovering the protocatechuate 2,3-cleavage pathway genes.

Journal

J Bacteriol 191:6758-68 (2009)
DOI:10.1128/JB.00840-09

Other DBs

ExplorEnz - The Enzyme Database: