KEGG   ENZYME: 4.2.1.170Help
Entry
EC 4.2.1.170                Enzyme                                 

Name
2-(omega-methylthio)alkylmalate dehydratase;
IPMI (gene name);
2-[(omega-methylthio)alkyl]malate hydro-lyase (2-[(omega-methylthio)alkyl]maleate-forming)
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
BRITE hierarchy
Sysname
2-[(omega-methylsulfanyl)alkyl]malate hydro-lyase (2-[(omega-methylsulfanyl)alkyl]maleate-forming)
Reaction(IUBMB)
(1) a 2-[(omega-methylsulfanyl)alkyl]malate = a 2-[(omega-methylsulfanyl)alkyl]maleate + H2O;
(2) a 3-[(omega-methylsulfanyl)alkyl]malate = a 2-[(omega-methylsulfanyl)alkyl]maleate + H2O
Reaction(KEGG)
Substrate
2-[(omega-methylsulfanyl)alkyl]malate;
3-[(omega-methylsulfanyl)alkyl]malate
Product
2-[(omega-methylsulfanyl)alkyl]maleate;
H2O [CPD:C00001]
Comment
The enzyme, characterized from the plant Arabidopsis thaliana, is involved in the L-methionine side-chain elongation pathway, forming substrates for the biosynthesis of aliphatic glucosinolates. By catalysing a dehydration of a 2-[(omega-methylsulfanyl)alkyl]maleate, followed by a hydration at a different position, the enzyme achieves the isomerization of its substrates. The enzyme is a heterodimer comprising a large and a small subunits. The large subunit can also bind to an alternative small subunit, forming EC 4.2.1.33, 3-isopropylmalate dehydratase, which participates in L-leucine biosynthesis.
History
EC 4.2.1.170 created 2016
Pathway
ec00966  Glucosinolate biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K21359  3-isopropylmalate/methylthioalkylmalate dehydratase small subunit
Genes
ATH: AT2G43100(IPMI2) AT3G58990(IPMI1)
ALY: ARALYDRAFT_483411 ARALYDRAFT_907390
CRB: 17886442 17889879 17895092
CSAT: 104781808 104782825 104785046 104788127 104792177 104793241
EUS: EUTSA_v10017125mg
BRP: 103866695
BNA: 106388301 106421092 106434491
BOE: 106339421
Taxonomy
Reference
1  [PMID:19597944]
  Authors
Knill T, Reichelt M, Paetz C, Gershenzon J, Binder S
  Title
Arabidopsis thaliana encodes a bacterial-type heterodimeric isopropylmalate isomerase involved in both Leu biosynthesis and the Met chain elongation pathway  of glucosinolate formation.
  Journal
Plant Mol Biol 71:227-39 (2009)
DOI:10.1007/s11103-009-9519-5
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.170
IUBMB Enzyme Nomenclature: 4.2.1.170
ExPASy - ENZYME nomenclature database: 4.2.1.170
BRENDA, the Enzyme Database: 4.2.1.170
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