KEGG   ENZYME: 4.4.1.28Help
Entry
EC 4.4.1.28                 Enzyme                                 

Name
L-cysteine desulfidase;
L-cysteine desulfhydrase
Class
Lyases;
Carbon-sulfur lyases;
Carbon-sulfur lyases (only sub-subclass identified to date)
BRITE hierarchy
Sysname
L-cysteine sulfide-lyase (deaminating; pyruvate-forming)
Reaction(IUBMB)
L-cysteine + H2O = sulfide + NH3 + pyruvate (overall reaction) [RN:R00782];
(1a) L-cysteine = 2-aminoprop-2-enoate + sulfide;
(1b) 2-aminoprop-2-enoate = 2-iminopropanoate (spontaneous);
(1c) 2-iminopropanoate + H2O = pyruvate + NH3 (spontaneous)
Reaction(KEGG)
Substrate
L-cysteine [CPD:C00097];
H2O [CPD:C00001];
2-aminoprop-2-enoate [CPD:C02218];
2-iminopropanoate [CPD:C20904]
Product
sulfide [CPD:C00283];
NH3 [CPD:C00014];
pyruvate [CPD:C00022];
2-aminoprop-2-enoate [CPD:C02218];
2-iminopropanoate [CPD:C20904]
Comment
The enzyme from the archaeon Methanocaldococcus jannaschii contains a [4Fe-4S] cluster and is specific for L-cysteine (cf. EC 4.4.1.1, cystathionine gamma-lyase). It cleaves a carbon-sulfur bond releasing sulfide and the unstable enamine product 2-aminoprop-2-enoate that tautomerizes to an imine form, which undergoes a hydrolytic deamination to form pyruvate and ammonia. The same reaction can also be catalysed by some pyridoxal-phosphate proteins (cf. EC 4.4.1.1, cystathionine gamma-lyase).
History
EC 4.4.1.28 created 2014
Pathway
ec00270  Cysteine and methionine metabolism
ec01100  Metabolic pathways
Orthology
K20021  L-cysteine desulfidase
K22207  L-cysteine desulfhydrase
Genes
SKO: 100366607
AQU: 100634704 100642116 105315073
ATH: AT3G62130(LCD) AT5G26600
CRB: 17882306 17885855
CSAT: 104699663 104706986 104736694 104749103 104788528
EUS: EUTSA_v10004144mg EUTSA_v10005965mg
BRP: 103830344 103874481
BNA: 106348582 106348715 106353308 111211374
BOE: 106300027 106306993
RSZ: 108817289 108833893 108854538
CPAP: 110815337
LJA: Lj0g3v0131109.1(Lj0g3v0131109.1) Lj0g3v0330309.1(Lj0g3v0330309.1) Lj1g3v4807370.1(Lj1g3v4807370.1)
CSV: 101221312
CMO: 103493954
MCHA: 111023288
BVG: 104890630
SOE: 110797240
DOSA: Os01t0290100-01(Os01g0290100) Os01t0290600-00(Os01g0290600)
BDI: 100844459
ATS: 109769734(LOC109769734) 109784901(LOC109784901)
DCT: 110101195
PEQ: 110029309
AOF: 109846945
ATR: 18430133
MIS: MICPUN_113236(LOLT)
MJA: MJ_1025
MMP: MMP1468
MMD: GYY_08175
 » show all
Taxonomy
Reference
1  [PMID:15683250]
  Authors
Tchong SI, Xu H, White RH
  Title
L-cysteine desulfidase: an [4Fe-4S] enzyme isolated from Methanocaldococcus jannaschii that catalyzes the breakdown of L-cysteine into pyruvate, ammonia, and sulfide.
  Journal
Biochemistry 44:1659-70 (2005)
DOI:10.1021/bi0484769
  Sequence
[mja:MJ_1025]
Other DBs
ExplorEnz - The Enzyme Database: 4.4.1.28
IUBMB Enzyme Nomenclature: 4.4.1.28
ExPASy - ENZYME nomenclature database: 4.4.1.28
BRENDA, the Enzyme Database: 4.4.1.28
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