KEGG   ENZYME: 4.4.1.29Help
Entry
EC 4.4.1.29                 Enzyme                                 

Name
phycobiliprotein cysteine-84 phycobilin lyase;
cpcS (gene name);
cpeS (gene name);
cpcS1 (gene name);
cpcU (gene name);
phycocyanobilin:Cys-beta84-phycobiliprotein lyase
Class
Lyases;
Carbon-sulfur lyases;
Carbon-sulfur lyases (only sub-subclass identified to date)
BRITE hierarchy
Sysname
[phycobiliprotein]-Cys84-phycobilin:phycobilin lyase
Reaction(IUBMB)
(1) [C-phycocyanin beta-subunit]-Cys84-phycocyanobilin = apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin;
(2) [phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin = apo-[phycoerythrocyanin beta-subunit] + (2R,3E)-phycocyanobilin;
(3) [allophycocyanin alpha-subunit]-Cys84-phycocyanobilin = apo-[allophycocyanin alpha-subunit] + (2R,3E)-phycocyanobilin;
(4) [allophycocyanin beta-subunit]-Cys84-phycocyanobilin = apo-[allophycocyanin beta-subunit] + (2R,3E)-phycocyanobilin;
(5) [C-phycoerythrin alpha-subunit]-Cys84-phycoerythrobilin = apo-[C-phycoerythrin alpha-subunit] + (2R,3E)-phycoerythrobilin;
(6) [C-phycoerythrin beta-subunit]-Cys84-phycoerythrobilin = apo-[C-phycoerythrin beta-subunit] + (2R,3E)-phycoerythrobilin
Substrate
[C-phycocyanin beta-subunit]-Cys84-phycocyanobilin;
[phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin;
[allophycocyanin alpha-subunit]-Cys84-phycocyanobilin;
[allophycocyanin beta-subunit]-Cys84-phycocyanobilin;
[C-phycoerythrin alpha-subunit]-Cys84-phycoerythrobilin;
[C-phycoerythrin beta-subunit]-Cys84-phycoerythrobilin
Product
apo-[C-phycocyanin beta-subunit];
(2R,3E)-phycocyanobilin;
apo-[phycoerythrocyanin beta-subunit];
apo-[allophycocyanin alpha-subunit];
apo-[allophycocyanin beta-subunit];
apo-[C-phycoerythrin alpha-subunit];
(2R,3E)-phycoerythrobilin;
apo-[C-phycoerythrin beta-subunit]
Comment
The enzyme, found in cyanobacteria and red algae, catalyses the attachment of phycobilin chromophores to cysteine 84 of several phycobiliproteins (the numbering used here corresponds to the enzyme from Anabaena, in other organisms the number may vary slightly). It can attach phycocyanobilin to the beta subunits of C-phycocyanin and phycoerythrocyanin and to both subunits of allophycocyanin. In addition, it can attach phycoerythrobilin to both subunits of C-phycoerythrin.
History
EC 4.4.1.29 created 2015
Reference
1  [PMID:16452471]
  Authors
Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H
  Title
Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120.
  Journal
J Biol Chem 281:8573-81 (2006)
DOI:10.1074/jbc.M513796200
  Sequence
[ana:alr0617]
Reference
2  [PMID:17726096]
  Authors
Zhao KH, Su P, Tu JM, Wang X, Liu H, Ploscher M, Eichacker L, Yang B, Zhou M, Scheer H
  Title
Phycobilin:cystein-84 biliprotein lyase, a near-universal lyase for cysteine-84-binding sites in cyanobacterial phycobiliproteins.
  Journal
Proc Natl Acad Sci U S A 104:14300-5 (2007)
DOI:10.1073/pnas.0706209104
  Sequence
[ana:alr0617]
Reference
3  [PMID:18199753]
  Authors
Saunee NA, Williams SR, Bryant DA, Schluchter WM
  Title
Biogenesis of phycobiliproteins: II. CpcS-I and CpcU comprise the heterodimeric bilin lyase that attaches phycocyanobilin to CYS-82 OF beta-phycocyanin and CYS-81 of allophycocyanin subunits in Synechococcus sp. PCC 7002.
  Journal
J Biol Chem 283:7513-22 (2008)
DOI:10.1074/jbc.M708165200
Reference
4  [PMID:19864423]
  Authors
Kupka M, Zhang J, Fu WL, Tu JM, Bohm S, Su P, Chen Y, Zhou M, Scheer H, Zhao KH
  Title
Catalytic mechanism of S-type phycobiliprotein lyase: chaperone-like action and functional amino acid residues.
  Journal
J Biol Chem 284:36405-14 (2009)
DOI:10.1074/jbc.M109.056382
  Sequence
[ana:alr0617]
Other DBs
ExplorEnz - The Enzyme Database: 4.4.1.29
IUBMB Enzyme Nomenclature: 4.4.1.29
ExPASy - ENZYME nomenclature database: 4.4.1.29
BRENDA, the Enzyme Database: 4.4.1.29
LinkDB All DBs

DBGET integrated database retrieval system