KEGG   ENZYME: 4.4.1.32Help
Entry
EC 4.4.1.32                 Enzyme                                 

Name
C-phycocyanin alpha-cysteine-84 phycocyanobilin lyase;
cpcE (gene name);
cpcF (gene name)
Class
Lyases;
Carbon-sulfur lyases;
Carbon-sulfur lyases (only sub-subclass identified to date)
BRITE hierarchy
Sysname
[C-phycocyanin alpha-subunit]-Cys84-phycocyanobilin:(2R,3E)-phycocyanobilin lyase
Reaction(IUBMB)
[C-phycocyanin alpha-subunit]-Cys84-phycocyanobilin = apo-[C-phycocyanin alpha-subunit] + (2R,3E)-phycocyanobilin
Substrate
[C-phycocyanin alpha-subunit]-Cys84-phycocyanobilin
Product
apo-[C-phycocyanin alpha-subunit];
(2R,3E)-phycocyanobilin
Comment
The enzyme, characterized from the cyanobacterium Synechococcus elongatus PCC 7942, catalyses the covalent attachment of the phycobilin chromophore phycocyanobilin to cysteine 84 of the alpha subunit of the phycobiliprotein C-phycocyanin.
History
EC 4.4.1.32 created 2015
Orthology
K02288  phycocyanobilin lyase subunit alpha
K02289  phycocyanobilin lyase subunit beta
Genes
CME: CYME_CMJ043C CYME_CMJ044C
GSL: Gasu_13890 Gasu_61960
CCP: CHC_T00009149001
SYN: sll1051(cpcF) slr1878(cpcE)
SYZ: MYO_111170(cpcE) MYO_1760(cpcF)
SYY: SYNGTS_0076(cpcF) SYNGTS_1107(cpcE)
SYT: SYNGTI_0076(cpcF) SYNGTI_1107(cpcE)
SYS: SYNPCCN_0076(cpcF) SYNPCCN_1106(cpcE)
SYQ: SYNPCCP_0076(cpcF) SYNPCCP_1106(cpcE)
SYJ: D082_28370(cpcE) D082_35050(cpcF)
SYC: syc0493_c(cpcF) syc0494_c(cpcE)
SYG: sync_0485(cpcF) sync_0486(cpcE)
SYR: SynRCC307_2066(rpcE) SynRCC307_2067(rpcF)
SYX: SynWH7803_0476(rpcF) SynWH7803_0477(rpcE)
CYA: CYA_0216(cpcF) CYA_0217(cpcE-1) CYA_2040(cpcE-2)
CYB: CYB_0942(cpcE-1) CYB_0943(cpcF) CYB_2736(cpcE-2)
SYH: Syncc8109_0435(rpcF) Syncc8109_0436(rpcE)
SYNW: SynWH8103_02329(rpcE) SynWH8103_02330(rpcF)
TEL: tlr1961(cpcE) tlr1962(cpcF)
THN: NK55_06500(cpcE) NK55_06505(cpcF)
CYI: CBM981_1808(rpcE) CBM981_1809(cpcF)
HHG: XM38_016360(cpcF) XM38_016370(cpcE)
PSER: ABRG53_2398(cpcF) ABRG53_2399(cpcE)
AMR: AM1_C0118(cpcE) AM1_C0272(cpcF)
MAR: MAE_27950(cpcF) MAE_27970(cpcE)
MVZ: myaer102_04100(cpcF) myaer102_04190(cpcE)
CYL: AA637_00365(cpcE) AA637_07775(cpcF)
CYT: cce_2308(cpcF) cce_2656(cpcE)
ARP: NIES39_D02000(cpcF) NIES39_D02010(cpcE)
PAGH: NIES204_12100(cpcF) NIES204_12110(cpcE)
GVI: gvip185(cpcE) gvip186(cpcF)
GLJ: GKIL_4303(cpcE) GKIL_4304(cpcF)
ANA: alr0532(cpcE) alr0533(cpcF)
NON: NOS3756_52570(cpcE) NOS3756_52580(cpcF)
ANB: ANA_C11072(cpcE) ANA_C11073(cpcF)
NSP: BMF81_02855(cpcF) BMF81_02856(cpcE)
DOU: BMF77_00697(cpcE) BMF77_00698(cpcF)
 » show all
Taxonomy
Reference
1  [PMID:1495995]
  Authors
Fairchild CD, Zhao J, Zhou J, Colson SE, Bryant DA, Glazer AN
  Title
Phycocyanin alpha-subunit phycocyanobilin lyase.
  Journal
Proc Natl Acad Sci U S A 89:7017-21 (1992)
DOI:10.1073/pnas.89.15.7017
Reference
2  [PMID:8132596]
  Authors
Fairchild CD, Glazer AN
  Title
Oligomeric structure, enzyme kinetics, and substrate specificity of the phycocyanin alpha subunit phycocyanobilin lyase.
  Journal
J Biol Chem 269:8686-94 (1994)
Reference
3  [PMID:7524727]
  Authors
Bhalerao RP, Lind LK, Gustafsson P
  Title
Cloning of the cpcE and cpcF genes from Synechococcus sp. PCC 6301 and their inactivation in Synechococcus sp. PCC 7942.
  Journal
Plant Mol Biol 26:313-26 (1994)
DOI:10.1007/BF00039542
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 4.4.1.32
IUBMB Enzyme Nomenclature: 4.4.1.32
ExPASy - ENZYME nomenclature database: 4.4.1.32
BRENDA, the Enzyme Database: 4.4.1.32
LinkDB All DBs

DBGET integrated database retrieval system