KEGG   ENZYME: 4.4.1.43
Entry
EC 4.4.1.43                 Enzyme                                 
Name
canavanine-gamma-lyase;
CangL
Class
Lyases;
Carbon-sulfur lyases;
Carbon-sulfur lyases (only sub-subclass identified to date)
Sysname
L-canavanine N-hydroxyguanidine-lyase (L-homoserine-forming)
Reaction(IUBMB)
L-canavanine + H2O = L-homoserine + N-hydroxyguanidine (overall reaction) [RN:R13115];
(1a) L-canavanine = vinylglycine + N-hydroxyguanidine [RN:R13112];
(1b) vinylglycine = (2E)-2-aminobut-2-enoate (spontaneous) [RN:R13113];
(1c) (2E)-2-aminobut-2-enoate + H2O = L-homoserine (spontaneous) [RN:R13114]
Reaction(KEGG)
R13112 R13113 R13114 R13115
Substrate
L-canavanine [CPD:C00308];
H2O [CPD:C00001];
vinylglycine [CPD:C22624];
(2E)-2-aminobut-2-enoate [CPD:C22625]
Product
L-homoserine [CPD:C00263];
N-hydroxyguanidine [CPD:C22626];
vinylglycine [CPD:C22624];
(2E)-2-aminobut-2-enoate [CPD:C22625]
Comment
A pyridoxal 5'-phosphate protein. The enzyme, characterized from the bacterium Pseudomonas canavaninivorans, cleaves a carbon-oxygen bond, releasing N-hydroxyguanidine and an unstable enamine product that tautomerizes to an imine form, which is attacked by a water molecule to form L-homoserine.
History
EC 4.4.1.43 created 2022
Orthology
K26365  canavanine-gamma-lyase
Genes
VNA: PN96_03100
VEJ: VEJY3_11085
VFU: vfu_B00618
VFL: AL536_01610
VGA: BSQ33_15705
VSR: Vspart_01493(mccB_1)
VZI: G5S32_19085
VOS: KNV97_03150
VRU: RND59_14575
PFE: PSF113_2762
PFB: VO64_0211
PTV: AA957_03515
PFF: PFLUOLIPICF723550
PBA: PSEBR_a3032
PSIL: PMA3_09645
PKE: DLD99_06065
PASG: KSS96_14595
PALV: KSS97_16230
PZA: HU749_014965
PCAS: LOY40_15610
PPAE: LDL65_25045
TAU: Tola_1635
BCED: DM42_6464(megL)
BMEC: WJ16_10180
PTER: C2L65_45090
PBRY: NDK50_07405
HYL: LPB072_00070
RGE: RGE_14420(metB)
SNN: EWH46_17835
MAMO: A6B35_32590
MHUA: MCHK_11665 MCHK_7242
RHI: NGR_b03470(metB)
SFH: SFHH103_06399(metB)
SFD: USDA257_c26390(mdeA2)
SAME: SAMCFNEI73_pC1887(mdeA)
SINO: SS05631_b52320
ESJ: SJ05684_b47920
EAK: EKH55_4889 EKH55_5636 EKH55_5829
ENU: PYH37_000757
AGC: BSY240_1706(megL)
ALF: CFBP5473_23990
RIR: BN877_p0249(metB)
REP: IE4803_CH03897
REI: IE4771_CH03835
RLT: Rleg2_5066
RPHA: AMC79_PD00749
RHK: Kim5_CH03866
RAD: CO657_28695
RROS: D4A92_12050
RPA: TX73_013590
RPT: Rpal_2896
MOC: BB934_32625
RVA: Rvan_3389
RLAC: QMO75_13665
NOH: G5V57_22075
SIL: SPO0413
RUT: FIU92_00790(mdeA1)
SULI: C1J05_17700
RHP: LPB142_12680
GEH: HYN69_15390
GFU: KM031_11000
NSM: JO391_10470
RMT: IAI58_05215
RCV: PFY06_20375
RAP: RHOA_0180
RRU: Rru_A0098
RRF: F11_00495
AFW: Anae109_4085
SUR: STAUR_0514
AVM: JQX13_40695
CFUS: CYFUS_004892
CKL: CKL_1632(metB2)
CKR: CKR_1517
CPAS: Clopa_2222
CPAT: CLPA_c22830(metB)
CPAE: CPAST_c22830(metB)
CLT: CM240_0811
CTYK: CTK_C14100(metB)
CDY: F3K33_11340
RAL: Rumal_2975
RUJ: E5Z56_00160
CAPR: EQM14_11875
SGY: Sgly_0928
ANV: RBQ60_09360
CLE: Clole_4164
CEW: EKH84_02320
ABUT: Ami103574_05840
TTO: Thethe_01586
MED: MELS_1685
MANA: MAMMFC1_00245(metB)
STED: SPTER_05340(megL)
CPRE: Csp1_01420(mgl_1)
GBR: Gbro_1986
GPO: GPOL_c18850
GOR: KTR9_1917
GOQ: ACH46_07535
GTA: BCM27_10300
GOC: CXX93_15170
GIT: C6V83_08050
GRU: GCWB2_09650(mdeA2)
GOM: D7316_00499(mgl_1)
GAV: C5O27_18105
GOD: GKZ92_09255
GAM: GII34_08940
GPD: GII33_08345
GJI: H1R19_09540
GOI: LK459_00775
GHN: MVF96_09690
GAMI: IHQ52_12540
GSI: P5P27_06125
DTM: BJL86_1088
TOY: FO059_15905
MIM: AKG07_14025
MIH: BJP65_12155
MHOS: CXR34_13240
MFOL: DXT68_01430
MCHN: HCR76_17005
MAZA: NFX31_02490
MRG: SM116_15510
AGM: DCE93_05400
CART: PA27867_1930
SALC: C2138_08010
SALD: FVA74_07670
LYD: D7I47_01150
MANT: BHD05_08840
AOG: LH407_12955
ARN: CGK93_10275
ORN: DV701_15935
BRI: FDF13_07530
AJI: C0Z10_12380
BROO: brsh051_18780
NBT: KLP28_12945
SACG: FDZ84_25750 FDZ84_35495
SVI: Svir_19090
PDX: Psed_3633
PSEE: FRP1_14915
PSEH: XF36_15135
PSEQ: AD006_22575
PECQ: AD017_02160
PHH: AFB00_00095
PAUT: Pdca_41470
PBRO: HOP40_23260
PPEL: H6H00_21940
KBU: Q4V64_03865
ACTY: OG774_00550
BLO: BL1559
BLB: BBMN68_1590
BLZ: BLGT_09155
BDE: BDP_0406(metB)
BDN: BBDE_0388
BLEM: BL8807_06145
BEU: BE0216_05485
BSUE: BS3272_00885
AGV: OJF2_75610(mdeA_2)
TPI: TREPR_1228
TAZ: TREAZ_1603
PPIO: CE91St28_02400
PYY: RAH42_12685
EVI: Echvi_4510
MARO: MarbSA_07340
MBN: Mboo_1995
MPL: Mpal_0648
MAX: MMALV_09500
MEUZ: KRP56_05405
 » show all
Reference
1  [PMID:36320885]
  Authors
Hauth F, Buck H, Stanoppi M, Hartig JS.
  Title
Canavanine utilization via homoserine and hydroxyguanidine by a PLP-dependent gamma-lyase in Pseudomonadaceae and Rhizobiales.
  Journal
RSC Chem Biol 3:1240-1250 (2022)
DOI:10.1039/d2cb00128d
  Sequence
[pcas:LOY40_15610]
Other DBs
ExplorEnz - The Enzyme Database: 4.4.1.43
IUBMB Enzyme Nomenclature: 4.4.1.43
ExPASy - ENZYME nomenclature database: 4.4.1.43
BRENDA, the Enzyme Database: 4.4.1.43
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