| Entry |
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| Name |
tRNA-intron lyase;
transfer ribonucleate intron endoribonuclease;
tRNA splicing endonuclease;
splicing endonuclease;
tRNATRPintron endonuclease;
transfer splicing endonuclease
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| Class |
Lyases;
Phosphorus-oxygen lyases;
Phosphorus-oxygen lyases (only sub-subclass identified to date)
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| Sysname |
pretRNA lyase (intron-removing; cyclic-2',3'-phosphate-forming)
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| Reaction(IUBMB) |
pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
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| Substrate |
pretRNA
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| Product |
3'-half-tRNA molecule with a 5'-OH end;
5'-half-tRNA molecule with a 2',3'-cyclic phosphate end;
intron with a 2',3'-cyclic phosphate-hydroxyl terminus;
a 5'-hydroxyl terminus
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| Comment |
The enzyme catalyses the final stage in the maturation of tRNA molecules.
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| History |
EC 4.6.1.16 created 1992 as EC 3.1.27.9, transferred 2014 to EC 4.6.1.16
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| Orthology |
| K01170 | tRNA-intron endonuclease, archaea type |
| K15322 | tRNA-splicing endonuclease subunit Sen2 |
| K15323 | tRNA-splicing endonuclease subunit Sen34 |
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| Genes |
» show all
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| Reference |
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| Authors |
Attardi DG, Margarit I, Tocchini-Valentini GP. |
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| Title |
Structural alterations in mutant precursors of the yeast tRNALeu3 gene which behave as defective substrates for a highly purified splicing endoribonuclease. |
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| Journal |
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| Reference |
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| Authors |
Peebles CL, Gegenheimer P, Abelson J. |
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| Title |
Precise excision of intervening sequences from precursor tRNAs by a membrane-associated yeast endonuclease. |
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| Journal |
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| Reference |
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| Authors |
Thompson LD, Brandon LD, Nieuwlandt DT, Daniels CJ. |
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| Title |
Transfer RNA intron processing in the halophilic archaebacteria. |
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| Journal |
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| Reference |
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| Authors |
Thompson LD, Daniels CJ. |
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| Title |
A tRNA(Trp) intron endonuclease from Halobacterium volcanii. Unique substrate recognition properties. |
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| Journal |
J Biol Chem 263:17951-9 (1988) |
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| Other DBs |
| ExplorEnz - The Enzyme Database: | 4.6.1.16 |
| ExPASy - ENZYME nomenclature database: | 4.6.1.16 |
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| LinkDB |
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