KEGG ENZYME: 5.3.3.1

ENZYME: 5.3.3.1

Entry

EC 5.3.3.1 Enzyme

Name

steroid Delta-isomerase;
hydroxysteroid isomerase;
steroid isomerase;
Delta5-ketosteroid isomerase;
Delta5(or Delta4)-3-keto steroid isomerase;
Delta5-steroid isomerase;
3-oxosteroid isomerase;
Delta5-3-keto steroid isomerase;
Delta5-3-oxosteroid isomerase

Class

Isomerases;
Intramolecular oxidoreductases;
Transposing C=C bonds

Sysname

3-oxosteroid Delta5-Delta4-isomerase

Reaction(IUBMB)

a 3-oxo-Delta5-steroid = a 3-oxo-Delta4-steroid [RN:R02644]

Reaction(KEGG)

R02644 > R09955;
(other) R01837 R02216 R02499 R02840 R03327 R04163 R04678 R04849 R09920

Substrate

3-oxo-Delta5-steroid [CPD:C01034]

Product

3-oxo-Delta4-steroid [CPD:C00619]

Comment

This activity is catalysed by several distinct enzymes (cf. EC 1.1.3.6, cholesterol oxidase and EC 1.1.1.145, 3-hydroxy-5-steroid dehydrogenase).

History

EC 5.3.3.1 created 1961

Pathway

ec00140

Steroid hormone biosynthesis

ec00984

Steroid degradation

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K00070

3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase

K01822

steroid Delta-isomerase

K16045

3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase

Genes

HSA:	3283(HSD3B1) 3284(HSD3B2)

PTR:	457165 469446

PPS:	100982464(HSD3B2) 100983009 100990187

GGO:	101141801 101146563(HSD3B2)

PON:	100451655 100452394

NLE:	100596087 100605995(HSD3B2) 100606563 100607955

MCC:	712686(HSD3B2) 713091

MCF:	102129497 102133592(HSD3B1) 102134747 102135110

CSAB:

103224100(HSD3B1) 103224104 103224107 103224109(HSD3B2)

RRO:	104654599 104654605

RBB:	108528160 108528161 108528164

CJC:	100387484 100389654(HSD3B1) 100400416 100405879

SBQ:	101042680 101051416

MMU:	100043456(Gm10681) 15492(Hsd3b1) 15493(Hsd3b2) 15495(Hsd3b4) 15496(Hsd3b5) 15497(Hsd3b6)

MCAL:

110287720 110288898 110290813 110290978 110291292 110291371 110291888

MPAH:

110315378 110319834 110319836 110320478 110320490

RNO:	108348086 24470(Hsd3b5) 29632(Hsd3b2) 360348(Hsd3b1) 682974(Hsd3b3)

MUN:	110542496 110543775 110544350 110544885 110563733 110563735

CGE:	100770922 113830678 113833179 113833198

NGI:	103728305 103732007 103738837

HGL:	101707488(Hsd3b1) 101707858

CCAN:

109702728 109702729

OCU:	100346986 100347914

TUP:	102470981 102471410

CFA:	483146(HSD3B2)

VVP:

112916789

AML:	100464280(HSD3B1)

UMR:

103677123

UAH:

113244362

ORO:

101378904

FCA:	100616070(HSD3B2)

PTG:

102957262

PPAD:

109272160

AJU:

106978496

BTA:	281824(HSD3B1)

BOM:

102274088

BIU:

109555859

BBUB:

102413429

CHX:	100860869(3BHSD)

OAS:	100190978(HSD3B1)

SSC:	445539(HSD3B1)

CFR:

CDK:

BACU:

LVE:

OOR:

DLE:

PCAD:

ECB:	100034078(HSD3B2)

EPZ:

103548379

EAI:	106832920(HSD3B2)

MYB:	106723550(HSD3B2)

MNA:	107545288(HSD3B2)

HAI:	109389868(HSD3B2)

DRO:	112314004(HSD3B2)

PALE:

102891879

RAY:	107518830(HSD3B2)

MJV:

108390800

LAV:	100659049(HSD3B2)

TMU:

101358325

MDO:

100012668

SHR:	100913530(HSD3B2)

PCW:	110219672(HSD3B2)

OAA:

100080135

GGA:	396015(HSD3B1)

MGP:

104915630

CJO:	107319373(3beta-HSD)

NMEL:

110398845

APLA:

101803865

ACYG:

106045741

TGU:	751770(HSD3B1)

LSR:

SCAN:

GFR:

FAB:

PHI:

PMAJ:

CCAE:

CCW:

ETL:

FPG:

FCH:

CLV:

EGZ:

NNI:

ACUN:

PADL:

AAM:

ASN:	102367786(HSD3B1)

AMJ:	109280168(HSD3B1)

PSS:

102453952

CMY:

102934871

CPIC:

101946936

ACS:	100561899(hsd3b1)

PVT:

PBI:

PMUR:

TSR:

PMUA:

114587929(HSD3B2)

GJA:

107114856

XLA:	734818(hsd3b1.L)

XTR:

100490622

NPR:

108802532

DRE:	373131(hsd3b2) 553532(hsd3b1)

SRX:

SANH:

SGH:

CCAR:

IPU:	100304475(HSD3B)

PHYP:

113524942(hsd3b1)

AMEX:

103027852(hsd3b1)

EEE:

113587753

TRU:	101069884(hsd3b1)

TNG:	GSTEN00029150G001

LCO:	104921488(hsd3b1)

MZE:

101485719

ONL:

100534568

OLA:	100144358(3bhsd)

XMA:

XCO:

PRET:

CVG:

NFU:	107389612(hsd3b1)

KMR:	108244192(hsd3b1)

ALIM:

106531920

AOCE:

111570570(hsd3b1)

CSEM:

103391994(hsd3b1)

POV:

LCF:

SDU:

SLAL:

HCQ:

BPEC:

110156192(hsd3b1)

MALB:

SASA:

OTW:

SALP:

ELS:

SFM:

PKI:

LCM:

CMK:

RTP:

BFO:	BRAFLDRAFT_221648

DME:	Dmel_CG7724(CG7724)

DER:

6543694

DSE:	Dsec_GM25688

DSI:	Dsimw501_GD14693(Dsim_GD14693)

DYA:	Dyak_GE19901

DAN:	Dana_GF10105 Dana_GF19644

DSR:	110187899 110190103

DPO:	Dpse_GA20545

DPE:

6603899

DMN:

108165559

DWI:

6645028

DGR:	Dgri_GH16404

DMO:	Dmoj_GI12485

DAZ:

108612818

DNV:

108651018

DHE:

111602930

DVI:	Dvir_GJ12388

MDE:

101899660

LCQ:

111691019

AGA:	AgaP_AGAP006654

AAG:

5566270

AALB:

109407756 109418935

CQU:	CpipJ_CPIJ003056

NVI:

CSOL:

CLEC:

ZNE:

FCD:

PVM:

ISC:	IscW_ISCW018715

TUT:

DPTE:

CSCU:

PTEP:

HRO:	HELRODRAFT_89375

CRG:	105332656 105336240

MYI:

110457155

LAK:

106151343

RGL:	CS053_01865

PRE:	PCA10_13780(ksi)

PVR:	PverR02_11605

PPUU:

PKC:

PSET:

SPL:

SHL:

CBER:

SPOI:

ZAL:	AZF00_08390

OSG:	BST96_05805

HJA:	BST95_12875 BST95_17215

KAK:	Kalk_07505

SDF:	ACG33_03815

SOK:	D0B54_00185 D0B54_09480

GPB:	HDN1F_02730(ksi)

REU:	Reut_A1514

REH:	H16_B0602(h16_B0602)

CNC:	CNE_2c05470

CTI:	RALTA_B0406

CBW:	RR42_s2306

COX:	E0W60_08050

BFN:

OI25_4583

AJS:

Ajs_2963

DIA:	Dtpsy_2390

CTT:	CtCNB1_1323

CTES:

CSER:

AZO:

XAU:

HNE:

NAR:	Saro_3596 Saro_3765

NPP:	PP1Y_Mpl2956

NOV:	TQ38_027200 TQ38_027400

NOT:	C7W88_20825 C7W88_20845

NTD:	EGO55_02335 EGO55_06035 EGO55_07020 EGO55_13785 EGO55_15950

SWI:

Swit_3295

SPHD:

HY78_09075

SPHM:

G432_01540 G432_04695 G432_04850

SSAN:

NX02_14810

SPKC:

KC8_00670 KC8_01015 KC8_01175 KC8_03160 KC8_10955 KC8_13360

SPHA:

D3Y57_15405

SPHB:

EP837_02960 EP837_03245 EP837_03374

SPHG:

AZE99_02185

SFLA:

SPHFLASMR4Y_01939(ksi)

SPHY:

CHN51_03380

ERK:	CD351_14410

PHZ:	CHX26_05225

OIH:

OB0288

MTU:	Rv0760c Rv1106c

MTV:	RVBD_0760c RVBD_1106c

MTC:	MT0785 MT1137

MRA:	MRA_0770 MRA_1117

MTF:	TBFG_10775 TBFG_11128

MTB:	TBMG_00775 TBMG_02877

MTK:	TBSG_00779 TBSG_02893

MTZ:	TBXG_000768 TBXG_002857

MTG:	MRGA327_04735 MRGA327_06925

MTI:	MRGA423_04755 MRGA423_06915

MTE:	CCDC5079_0703 CCDC5079_1022

MTUR:

CFBS_0799 CFBS_1174

MTL:	CCDC5180_0695 CCDC5180_1014

MTO:	MTCTRI2_0779 MTCTRI2_1136

MTD:	UDA_0760c UDA_1106c

MTN:	ERDMAN_0842 ERDMAN_1239

MTJ:	J112_04090 J112_05975

MTUB:

MT7199_0781 MT7199_1133

MTUC:

J113_05370 J113_07785

MTUE:

J114_04060 J114_05980

MTX:	M943_03980 M943_05820

MTUH:

I917_05390 I917_07855

MTUL:

TBHG_00752 TBHG_01091

MTUT:

HKBT1_0799 HKBT1_1172

MTUU:

HKBT2_0800 HKBT2_1178

MTQ:	HKBS1_0799 HKBS1_1174

MBO:	BQ2027_MB0783C BQ2027_MB1136C

MBB:	BCG_0812c BCG_1166c

MBT:	JTY_0782 JTY_1139

MBM:	BCGMEX_0783c BCGMEX_1138c

MBK:	K60_008130 K60_011910

MBX:	BCGT_0561 BCGT_0934

MAF:	MAF_07720 MAF_11210

MMIC:

RN08_0849 RN08_1242

MCE:	MCAN_07641 MCAN_11161

MCQ:	BN44_10830 BN44_11231

MCV:	BN43_20195 BN43_30163

MCX:	BN42_20518 BN42_20954

MCZ:	BN45_20029 BN45_30150

MLE:

ML1942

MLB:

MLBr01942

MPA:	MAP_0594c MAP_2478 MAP_2688 MAP_2705c

MAO:	MAP4_1111 MAP4_1128 MAP4_1345 MAP4_3271

MAVI:

RC58_05520 RC58_05605 RC58_06655 RC58_16235

MAVU:

RE97_05515 RE97_05600 RE97_06645 RE97_16265

MAV:	MAV_0704 MAV_1209 MAV_1225 MAV_1444

MIT:	OCO_05970 OCO_11160 OCO_11340 OCO_15430

MIA:	OCU_06010 OCU_11140 OCU_11320 OCU_15870

MID:	MIP_01077 MIP_01799 MIP_01824 MIP_02150

MYO:	OEM_06060 OEM_11270 OEM_11450 OEM_13520

MIR:	OCQ_06120 OCQ_11170 OCQ_11350 OCQ_13340

MCHI:

AN480_02980 AN480_06660 AN480_06750 AN480_07710

MMAL:

CKJ54_02920 CKJ54_05425 CKJ54_05520 CKJ54_06510

MLP:	MLM_0827 MLM_1173 MLM_1377

MSA:	Mycsm_05045 Mycsm_05075 Mycsm_05686

MUL:	MUL_0172 MUL_0195 MUL_0468

MMC:	Mmcs_4109 Mmcs_4139 Mmcs_4586

MKM:	Mkms_4185 Mkms_4214 Mkms_4674

MJL:	Mjls_4340 Mjls_4370 Mjls_4969

MMI:	MMAR_4359 MMAR_4382 MMAR_4937

MMAE:

MMARE11_41790 MMARE11_42020 MMARE11_47470

MMM:	W7S_02930 W7S_05455 W7S_05545 W7S_06520

MLI:	MULP_04570 MULP_04597 MULP_05181

MKN:	MKAN_07985 MKAN_08100 MKAN_11300

MYV:	G155_04240 G155_05080 G155_22700 G155_22900

MYE:	AB431_06300 AB431_06460 AB431_26000

MHAD:

B586_05030 B586_07090

MYN:	MyAD_21005 MyAD_21100 MyAD_23425

MDX:	BTO20_08205 BTO20_08365 BTO20_30850

MSHG:

MSG_03923 MSG_03940 MSG_04437

MSM:	MSMEG_5228 MSMEG_5258 MSMEG_5867

MSG:	MSMEI_5091 MSMEI_5120 MSMEI_5708

MSB:	LJ00_25860 LJ00_26005 LJ00_29015

MSN:	LI99_25865 LI99_26010 LI99_29020

MSH:	LI98_25870 LI98_26015 LI98_29025

MVA:	Mvan_4635 Mvan_4664 Mvan_5165

MGI:	Mflv_1592 Mflv_2053 Mflv_2075

MSP:	Mspyr1_09780 Mspyr1_14770 Mspyr1_14990

MCB:	Mycch_4050 Mycch_4076 Mycch_4526

MNE:	D174_21420 D174_21515 D174_23880

MGO:	AFA91_11385 AFA91_14335 AFA91_14490

MFT:	XA26_08050 XA26_09800 XA26_46570 XA26_46970

MPHL:

MPHLCCUG_00647 MPHLCCUG_04229 MPHLCCUG_04255(ksi)

MVQ:	MYVA_4444 MYVA_4490 MYVA_5047

MLL:	B1R94_06275 B1R94_06430 B1R94_24625

MRH:	MycrhN_2406 MycrhN_3525 MycrhN_3550

MTHN:

4412656_03537 4412656_03575(ksi_2) 4412656_03992

MHAS:

MHAS_03213 MHAS_03236(ksi) MHAS_03651

MAB:

MAB_1206

MMV:	MYCMA_0644

MABB:

MASS_1204

MABL:

MMASJCM_1233

MCHE:

BB28_05990

MIZ:	BAB75_06575

MSTE:

MSTE_01173

MSAO:

MYCSP_05320

MSAL:

DSM43276_01073(ksi)

MJD:	JDM601_1031 JDM601_1056

MTER:

4434518_01017(ksi_1) 4434518_01040

ASD:

AS9A_0954

NFA:	NFA_13370 NFA_22430

NFR:	ERS450000_01854(ksi_1) ERS450000_02770(ksi_2)

NCY:	NOCYR_1399(ksdI) NOCYR_2411

NBR:	O3I_008870 O3I_012175

NNO:	NONO_c36120 NONO_c59430(ksdI)

NSL:	BOX37_11780

NSR:	NS506_06879

NOZ:	DMB37_29655

NOD:	FOH10_22975

RHA:	RHA1_ro03609 RHA1_ro09001

RER:	RER_13740(ksi) RER_59940

REY:	O5Y_06470 O5Y_28730

REB:	XU06_06730 XU06_08140 XU06_29060

ROP:	ROP_34080(ksi)

ROA:	Pd630_LPD08001

REQ:	REQ_00120 REQ_46990

RPY:	Y013_10750 Y013_14870 Y013_16310

RAV:	AAT18_20785 AAT18_26480

RHW:	BFN03_18225

RRZ:	CS378_10130 CS378_15850

RQI:	C1M55_06975 C1M55_30540

RHQ:	IM25_02490 IM25_06195

RHOD:

AOT96_10190 AOT96_17370

RRT:	4535765_04040(ksi_1) 4535765_04891(ksi_2)

RBY:	CEJ39_02755 CEJ39_21545

GBR:

Gbro_4817

GPO:	GPOL_c41280(ksdI) GPOL_c49840

GOR:

KTR9_4702

GOQ:	ACH46_03070 ACH46_20535

GTA:	BCM27_04880 BCM27_24895

GOC:	CXX93_03165

GIT:	C6V83_01325

GRU:	GCWB2_07285(ksi1) GCWB2_23650(ksi2)

GOM:	D7316_02575(ksi_1) D7316_03579(ksi_2)

GAV:	C5O27_04875 C5O27_20520

DTM:	BJL86_3299

DIT:	C3V38_06125

DIZ:	CT688_16895

DPC:	A6048_17485

DLU:	A6035_16835

TOY:	FO059_05695 FO059_17810

SGR:

SGR_6072

SVL:	Strvi_3968

SALS:

SLNWT_0271

SRC:	M271_05600 M271_05605

STRC:

AA958_00655

SAUO:

BV401_39990

SMAL:

SMALA_7276 SMALA_7277

NCA:

Noca_3954

NDK:	I601_0503(ksi)

PSIM:

KR76_23530 KR76_27120

FAL:

SEN:

AMYC:

SAQ:

CYN:	Cyan7425_3259

MIC:	Mic7113_3670

MPRO:

BJP34_04745

NON:	NOS3756_37870

NOE:	CLI64_13960

CALO:

Cal7507_5935

RIV:	Riv7116_4576 Riv7116_5342

FIS:	FIS3754_26380

TRS:	Terro_1090

NMV:	NITMOv2_2424

» show all

Reference

1 [PMID:5867327]

Authors

Ewald W, Werbin H, Chaikoff IL.

Title

Evidence for the presence of 17-hydroxypregnenedione isomerase in beef adrenal cortex.

Journal

Biochim Biophys Acta 111:306-12 (1965)
DOI:10.1016/0304-4165(65)90497-6

Reference

2 [PMID:14404954]

Authors

KAWAHARA FS, TALALAY P.

Title

Crystalline Delta 5-3-ketosteroid isomerase.

Journal

J Biol Chem 235:PC1-2 (1960)

Reference

3 [PMID:13276386]

Authors

TALALAY P, WANG VS.

Title

Enzymic isomerization of delta5-3-ketosteroids.

Journal

Biochim Biophys Acta 18:300-1 (1955)

Reference

4 [PMID:10822008]

Authors

MacLachlan J, Wotherspoon AT, Ansell RO, Brooks CJ

Title

Cholesterol oxidase: sources, physical properties and analytical applications.

Journal

J Steroid Biochem Mol Biol 72:169-95 (2000)
DOI:10.1016/S0960-0760(00)00044-3

Other DBs

ExplorEnz - The Enzyme Database:

5.3.3.1

IUBMB Enzyme Nomenclature:

5.3.3.1

ExPASy - ENZYME nomenclature database:

5.3.3.1

BRENDA, the Enzyme Database:

5.3.3.1

CAS:

9031-36-1

LinkDB

DBGET integrated database retrieval system