Ligases;
Forming carbon-oxygen bonds;
Ligases forming aminoacyl-tRNA and related compounds
BRITE hierarchy

L-pyrrolysine:tRNAPyl ligase (AMP-forming)

ATP + L-pyrrolysine + tRNAPyl = AMP + diphosphate + L-pyrrolysyl-tRNAPyl [RN:R08578]

R08578
Reaction

ATP [CPD:C00002];
L-pyrrolysine [CPD:C16138];
tRNA(Pyl) [CPD:C16139]

In organisms such as Methanosarcina barkeri that incorporate the modified amino acid pyrrolysine (Pyl) into certain methylamine methyltransferases, an unusual tRNAPyl, with a CUA anticodon, can be charged directly with pyrrolysine by this class II aminoacyl---tRNA ligase. The enzyme is specific for pyrrolysine as substrate as it cannot be replaced by lysine or any of the other natural amino acids [1].

EC 6.1.1.26 created 2007

1  [PMID:15329732]

Blight SK, Larue RC, Mahapatra A, Longstaff DG, Chang E, Zhao G, Kang PT, Green-Church KB, Chan MK, Krzycki JA.

Direct charging of tRNA(CUA) with pyrrolysine in vitro and in vivo.

Nature 431:333-5 (2004)
DOI:10.1038/nature02895

[mba:Mbar_A0839]

2  [PMID:15314242]

Polycarpo C, Ambrogelly A, Berube A, Winbush SM, McCloskey JA, Crain PF, Wood JL, Soll D

An aminoacyl-tRNA synthetase that specifically activates pyrrolysine.

Proc Natl Acad Sci U S A 101:12450-4 (2004)
DOI:10.1073/pnas.0405362101

[mba:Mbar_A0839]

3  [PMID:15372017]

Schimmel P, Beebe K.

Molecular biology: genetic code seizes pyrrolysine.

Nature 431:257-8 (2004)
DOI:10.1038/431257a