KEGG   ENZYME: 6.5.1.5
Entry
EC 6.5.1.5                  Enzyme                                 
Name
RNA 3'-terminal-phosphate cyclase (GTP);
Pf-Rtc;
RNA-3'-phosphate cyclase (GTP)
Class
Ligases;
Forming phosphoric-ester bonds;
Ligases that form phosphoric-ester bonds (only sub-subclass identified to date)
Sysname
RNA-3'-phosphate:RNA ligase (cyclizing, GMP-forming)
Reaction(IUBMB)
GTP + [RNA]-3'-(3'-phospho-ribonucleoside) = GMP + diphosphate + [RNA]-3'-(2',3'-cyclophospho)-ribonucleoside (overall reaction) [RN:R10537];
(1a) GTP + [RNA 3'-phosphate cyclase]-L-histidine = 5'-guanosyl [RNA 3'-phosphate cyclase]-Ntau-phosphono-L-histidine + diphosphate;
(1b) 5'-guanosyl [RNA 3'-phosphate cyclase]-Ntau-phosphono-L-histidine + [RNA]-3'-(3'-phospho-ribonucleoside) = [RNA 3'-phosphate cyclase]-L-histidine + [RNA]-3'-ribonucleoside-3'-(5'-diphosphoguanosine);
(1c) [RNA]-3'-ribonucleoside-3'-(5'-diphosphoguanosine) = [RNA]-3'-(2',3'-cyclophospho)-ribonucleoside + GMP
Reaction(KEGG)
R10537
Substrate
GTP [CPD:C00044];
[RNA]-3'-(3'-phospho-ribonucleoside);
[RNA 3'-phosphate cyclase]-L-histidine;
5'-guanosyl [RNA 3'-phosphate cyclase]-Ntau-phosphono-L-histidine;
[RNA]-3'-ribonucleoside-3'-(5'-diphosphoguanosine)
Product
GMP [CPD:C00144];
diphosphate [CPD:C00013];
[RNA]-3'-(2',3'-cyclophospho)-ribonucleoside;
5'-guanosyl [RNA 3'-phosphate cyclase]-Ntau-phosphono-L-histidine;
[RNA 3'-phosphate cyclase]-L-histidine;
[RNA]-3'-ribonucleoside-3'-(5'-diphosphoguanosine)
Comment
The enzyme, which is specific for GTP, was characterized from the archaeon Pyrococcus furiosus. The enzyme converts the 3'-terminal phosphate of various RNA substrates into the 2',3'-cyclic phosphodiester in a GTP-dependent reaction. Catalysis occurs by a three-step mechanism, starting with the activation of the enzyme by GTP, forming a phosphoramide bond between guanylate and a histidine residue. The guanylate group is then transferred to the 3'-phosphate terminus of the substrate, forming the capped structure [RNA]-3'-(5'-diphosphoguanosine). Finally, the enzyme catalyses an attack of the vicinal O-2' on the 3'-phosphorus, which results in formation of cyclic phosphate and release of the guanylate. cf. EC 6.5.1.4, RNA-3'-phosphate cyclase (ATP).
History
EC 6.5.1.5 created 2013, modified 2016
Orthology
K18105  RNA 3'-terminal phosphate cyclase (GTP)
Genes
THUGKNN16_13000(rtcA)
NPYNPRO_05470
TMAIFVE67_06470(rtcA)
MTHMFZP57_02120
MKAMK0001(rcl1)
PFUPF1549
PFIPFC_06975
PHOPH1529(PH1529)
PABPAB1943
PYNPNA2_0113
PYAPYCH_05910
PYSPy04_1420
PYCTQ32_09235
TKOTK1615
TONTON_0048
TGATGAM_2020(rtcA)
TSITSIB_1528
TBATERMP_00074
THEGQS_05015
THATAM4_6
THMCL1_0647
TLTOCC_03117
THSTES1_0173
TNUBD01_2074
TEUTEU_04715
TGYX802_03300
THVADU37_CDS16220
TCHCHITON_1756
TPEPA0127_07130
TPIEA7C91_06170
TGGA3K92_00565
TCEA3L02_00995
TBSA3L01_00895
THHCDI07_00890
TSLA3L11_04640
TTDA3L14_02295
TPRFA3L09_02395
TRLA3L10_05680
TPAFA3L08_03950
THYA3L12_00545
TICFH039_02715
TCQTIRI35C_0394(rtcA)
THEMFPV09_06605
PPACPAP_08005
MEAMMU439_01765(rtcA)
THELIG193_06520
 » show all
Reference
1  [PMID:22074260]
  Authors
Sato A, Soga T, Igarashi K, Takesue K, Tomita M, Kanai A
  Title
GTP-dependent RNA 3'-terminal phosphate cyclase from the hyperthermophilic archaeon Pyrococcus furiosus.
  Journal
Genes Cells 16:1190-9 (2011)
DOI:10.1111/j.1365-2443.2011.01561.x
  Sequence
[pfu:PF1549]
Other DBs
ExplorEnz - The Enzyme Database: 6.5.1.5
IUBMB Enzyme Nomenclature: 6.5.1.5
ExPASy - ENZYME nomenclature database: 6.5.1.5
BRENDA, the Enzyme Database: 6.5.1.5
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