KEGG   ENZYME: 1.5.4.1Help
Entry
EC 1.5.4.1                  Enzyme                                 

Name
pyrimidodiazepine synthase;
PDA synthase;
pyrimidodiazepine:oxidized-glutathione oxidoreductase (ring-opening, cyclizing);
pyrimidodiazepine:glutathione-disulfide oxidoreductase (ring-opening, cyclizing)
Class
Oxidoreductases;
Acting on the CH-NH group of donors;
With a disulfide as acceptor
BRITE hierarchy
Sysname
2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido[4,5-b][1,4]diazepin-4-one:glutathione-disulfide oxidoreductase (ring-opening, cyclizing)
Reaction(IUBMB)
2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido[4,5-b][1,4]diazepin-4-one + glutathione disulfide + H2O = 6-pyruvoyltetrahydropterin + 2 glutathione [RN:R03984]
Reaction(KEGG)
Substrate
2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido[4,5-b][1,4]diazepin-4-one [CPD:C02587];
glutathione disulfide [CPD:C00127];
H2O [CPD:C00001]
Product
6-pyruvoyltetrahydropterin [CPD:C03684];
glutathione [CPD:C00051]
Comment
In the reverse direction, the reduction of 6-pyruvoyl-tetrahydropterin is accompanied by the opening of the 6-membered pyrazine ring and the formation of the 7-membered diazepine ring. The pyrimidodiazepine formed is an acetyldihydro derivative. Involved in the formation of the eye pigment drosopterin in Drosophila melanogaster.
History
EC 1.5.4.1 created 1990, modified 2014
Pathway
ec00480  Glutathione metabolism
ec00790  Folate biosynthesis
Orthology
K00310  pyrimidodiazepine synthase
Genes
DME: Dmel_CG6673(GstO2) Dmel_CG6776(GstO3) Dmel_CG6781(se)
DPO: Dpse_GA19859
DAN: Dana_GF24331
DER: Dere_GG14321
DPE: 6601435
DSE: Dsec_GM25062
DSI: Dsimw501_GD14100(Dsim_GD14100)
DWI: 6645945
DYA: Dyak_GE20749
DGR: Dgri_GH15265
MDE: 101891696
AGA: AgaP_AGAP005749(GSTO1)
AAG: 23687505
AME: 552118
BIM: 100745560
SOC: 105200535
AEC: 105147922
HST: 105185767
CFO: 105256274
NVI: 100116224(GstO1)
TCA: 655757
BMOR: 692819(GSTo1) 732973(GSTo3)
 » show all
Taxonomy
Reference
1  [PMID:6438092]
  Authors
Wiederrecht GJ, Brown GM.
  Title
Purification and properties of the enzymes from Drosophila melanogaster that catalyze the conversion of dihydroneopterin triphosphate to the pyrimidodiazepine precursor of the drosopterins.
  Journal
J Biol Chem 259:14121-7 (1984)
Reference
2  [PMID:16712527]
  Authors
Kim J, Suh H, Kim S, Kim K, Ahn C, Yim J
  Title
Identification and characteristics of the structural gene for the Drosophila eye  colour mutant sepia, encoding PDA synthase, a member of the omega class glutathione S-transferases.
  Journal
Biochem J 398:451-60 (2006)
DOI:10.1042/BJ20060424
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.5.4.1
IUBMB Enzyme Nomenclature: 1.5.4.1
ExPASy - ENZYME nomenclature database: 1.5.4.1
BRENDA, the Enzyme Database: 1.5.4.1
CAS: 93586-06-2
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