KEGG   PATHWAY: azd03030
azd03030                    Pathway                                

DNA replication - Azoarcus sp. DN11
A complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (DnaB or MCM complex) precedes the DNA synthetic machinery and unwinds the duplex parental DNA in cooperation with the SSB or RPA. On the leading strand, replication occurs continuously in a 5 to 3 direction, whereas on the lagging strand, DNA replication occurs discontinuously by synthesis and joining of short Okazaki fragments. In prokaryotes, the leading strand replication apparatus consists of a DNA polymerase (pol III core), a sliding clamp (beta), and a clamp loader (gamma delta complex). The DNA primase (DnaG) is needed to form RNA primers. Normally, during replication of the lagging-strand DNA template, an RNA primer is removed either by an RNase H or by the 5 to 3 exonuclease activity of DNA pol I, and the DNA ligase joins the Okazaki fragments. In eukaryotes, three DNA polymerases (alpha, delta, and epsilon) have been identified. DNA primase forms a permanent complex with DNA polymerase alpha. PCNA and RFC function as a clamp and a clamp loader. FEN 1 and RNase H1 remove the RNA from the Okazaki fragments and DNA ligase I joins the DNA.
Genetic Information Processing; Replication and repair
Pathway map
azd03030  DNA replication

Azoarcus sp. DN11 [GN:azd]
CDA09_15835  DNA polymerase III subunit epsilon [KO:K02342] [EC:]
CDA09_11925  DNA polymerase III subunit epsilon [KO:K02342] [EC:]
CDA09_08950  DNA polymerase III subunit epsilon [KO:K02342] [EC:]
CDA09_01235  ethanolamine utilization protein [KO:K02342] [EC:]
CDA09_03780  DNA polymerase III subunit alpha [KO:K02337] [EC:]
CDA09_04610  DNA polymerase III subunit gamma/tau [KO:K02343] [EC:]
CDA09_15420  holB; DNA polymerase III subunit delta' [KO:K02341] [EC:]
CDA09_03730  DNA polymerase III subunit delta [KO:K02340] [EC:]
CDA09_08150  DNA polymerase III subunit chi [KO:K02339] [EC:]
CDA09_00015  DNA polymerase III subunit beta [KO:K02338] [EC:]
CDA09_02835  replicative DNA helicase [KO:K02314] [EC:]
CDA09_03675  DNA primase [KO:K02316] [EC:]
CDA09_19370  single-stranded DNA-binding protein [KO:K03111]
CDA09_13945  hypothetical protein [KO:K03111]
CDA09_22160  single-stranded DNA-binding protein [KO:K03111]
CDA09_11920  ribonuclease HI [KO:K03469] [EC:]
CDA09_07120  ribonuclease HII [KO:K03470] [EC:]
CDA09_02515  DNA polymerase I [KO:K02335] [EC:]
CDA09_14895  DNA ligase (NAD(+)) LigA [KO:K01972] [EC:]
Stillman B.
Smart machines at the DNA replication fork.
Cell 78:725-8 (1994)
Waga S, Stillman B.
The DNA replication fork in eukaryotic cells.
Annu Rev Biochem 67:721-51 (1998)
KO pathway

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