KEGG   PATHWAY: ppsc03030
ppsc03030                   Pathway                                

DNA replication - Paenibacillus psychroresistens
A complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (DnaB or MCM complex) precedes the DNA synthetic machinery and unwinds the duplex parental DNA in cooperation with the SSB or RPA. On the leading strand, replication occurs continuously in a 5 to 3 direction, whereas on the lagging strand, DNA replication occurs discontinuously by synthesis and joining of short Okazaki fragments. In prokaryotes, the leading strand replication apparatus consists of a DNA polymerase (pol III core), a sliding clamp (beta), and a clamp loader (gamma delta complex). The DNA primase (DnaG) is needed to form RNA primers. Normally, during replication of the lagging-strand DNA template, an RNA primer is removed either by an RNase H or by the 5 to 3 exonuclease activity of DNA pol I, and the DNA ligase joins the Okazaki fragments. In eukaryotes, three DNA polymerases (alpha, delta, and epsilon) have been identified. DNA primase forms a permanent complex with DNA polymerase alpha. PCNA and RFC function as a clamp and a clamp loader. FEN 1 and RNase H1 remove the RNA from the Okazaki fragments and DNA ligase I joins the DNA.
Genetic Information Processing; Replication and repair
Pathway map
ppsc03030  DNA replication

Paenibacillus psychroresistens [GN:ppsc]
EHS13_20045  3'-5' exonuclease [KO:K02342] [EC:]
EHS13_03955  3'-5' exonuclease [KO:K02342] [EC:]
EHS13_22365  3'-5' exonuclease [KO:K02342] [EC:]
EHS13_24405  DNA polymerase III subunit alpha [KO:K02337] [EC:]
EHS13_15960  PolC-type DNA polymerase III [KO:K03763] [EC:]
EHS13_32365  dnaX; DNA polymerase III subunit gamma/tau [KO:K02343] [EC:]
EHS13_01385  holB; DNA polymerase III subunit delta' [KO:K02341] [EC:]
EHS13_12780  holA; DNA polymerase III subunit delta [KO:K02340] [EC:]
EHS13_00010  dnaN; DNA polymerase III subunit beta [KO:K02338] [EC:]
EHS13_20075  replicative DNA helicase [KO:K02314] [EC:]
EHS13_13625  dnaB; replicative DNA helicase [KO:K02314] [EC:]
EHS13_35770  dnaB; replicative DNA helicase [KO:K02314] [EC:]
EHS13_13010  DNA primase [KO:K02316] [EC:]
EHS13_35910  ssb; single-stranded DNA-binding protein [KO:K03111]
EHS13_03425  ribonuclease H [KO:K03469] [EC:]
EHS13_15640  ribonuclease HII [KO:K03470] [EC:]
EHS13_23160  polA; DNA polymerase I [KO:K02335] [EC:]
EHS13_00935  ligA; NAD-dependent DNA ligase LigA [KO:K01972] [EC:]
Stillman B.
Smart machines at the DNA replication fork.
Cell 78:725-8 (1994)
Waga S, Stillman B.
The DNA replication fork in eukaryotic cells.
Annu Rev Biochem 67:721-51 (1998)
KO pathway

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