Entry
Name
erythromycin-D,NADPH:oxygen oxidoreductase (12-hydroxylating)
Definition
Erythromycin D + NADPH + H+ + Oxygen <=> Erythromycin C + NADP+ + H2O
Equation
Reaction class
Enzyme
Pathway
rn00522 Biosynthesis of 12-, 14- and 16-membered macrolides
rn01110 Biosynthesis of secondary metabolites
Module
M00774 Erythromycin biosynthesis, propanoyl-CoA + methylmalonyl-CoA => deoxyerythronolide B => erythromycin A/B
Brite
Enzymatic reactions [BR:br08201 ]
1. Oxidoreductase reactions
1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
1.14.13.154
R05522 Erythromycin D + NADPH + H+ + Oxygen <=> Erythromycin C + NADP+ + H2O
BRITE hierarchy
Orthology
Reference
Authors
Savino C, Montemiglio LC, Sciara G, Miele AE, Kendrew SG, Jemth P, Gianni S, Vallone B
Title
Investigating the structural plasticity of a cytochrome P450: three-dimensional structures of P450 EryK and binding to its physiological substrate.
Journal
Reference
Authors
Stassi D, Donadio S, Staver MJ, Katz L
Title
Identification of a Saccharopolyspora erythraea gene required for the final hydroxylation step in erythromycin biosynthesis.
Journal
Reference
Authors
Lambalot RH, Cane DE, Aparicio JJ, Katz L
Title
Overproduction and characterization of the erythromycin C-12 hydroxylase, EryK.
Journal
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