KEGG   REACTION: R05522
Entry
R05522                      Reaction                               
Name
erythromycin-D,NADPH:oxygen oxidoreductase (12-hydroxylating)
Definition
Erythromycin D + NADPH + H+ + Oxygen <=> Erythromycin C + NADP+ + H2O
Equation
Reaction class
RC00001  C00005_C00006
RC01892  C06616_C06633
Enzyme
Pathway
rn00522  Biosynthesis of 12-, 14- and 16-membered macrolides
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00774  Erythromycin biosynthesis, propanoyl-CoA + methylmalonyl-CoA => deoxyerythronolide B => erythromycin A/B
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.154
     R05522  Erythromycin D + NADPH + H+ + Oxygen <=> Erythromycin C + NADP+ + H2O
Orthology
K14370  erythromycin 12 hydroxylase [EC:1.14.13.154]
Reference
1  [PMID:19625248]
  Authors
Savino C, Montemiglio LC, Sciara G, Miele AE, Kendrew SG, Jemth P, Gianni S, Vallone B
  Title
Investigating the structural plasticity of a cytochrome P450: three-dimensional structures of P450 EryK and binding to its physiological substrate.
  Journal
J Biol Chem 284:29170-9 (2009)
DOI:10.1074/jbc.M109.003590
Reference
2  [PMID:8416893]
  Authors
Stassi D, Donadio S, Staver MJ, Katz L
  Title
Identification of a Saccharopolyspora erythraea gene required for the final hydroxylation step in erythromycin biosynthesis.
  Journal
J Bacteriol 175:182-9 (1993)
DOI:10.1128/JB.175.1.182-189.1993
Reference
3  [PMID:7849045]
  Authors
Lambalot RH, Cane DE, Aparicio JJ, Katz L
  Title
Overproduction and characterization of the erythromycin C-12 hydroxylase, EryK.
  Journal
Biochemistry 34:1858-66 (1995)
DOI:10.1021/bi00006a006
Other DBs
RHEA: 32634
LinkDB

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