KEGG   ENZYME: 1.1.1.356
Entry
EC 1.1.1.356                Enzyme                                 
Name
GDP-L-colitose synthase;
ColC
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
GDP-beta-L-colitose:NAD(P)+ 4-oxidoreductase (5-epimerizing)
Reaction(IUBMB)
GDP-beta-L-colitose + NAD(P)+ = GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose + NAD(P)H + H+ [RN:R10420 R10421]
Reaction(KEGG)
R10420 R10421
Substrate
GDP-beta-L-colitose [CPD:C20613];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006]
Product
GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose [CPD:C20612];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
The enzyme is involved in biosynthesis of L-colitose, a 3,6-dideoxyhexose found in the O-antigen of Gram-negative lipopolysaccharides, where it catalyses the reaction in the reverse direction. The enzyme also performs the NAD(P)H-dependent epimerisation at C-5 of the sugar. The enzyme from Yersinia pseudotuberculosis is Si-specific with respect to NAD(P)H [1].
History
EC 1.1.1.356 created 2013
Pathway
ec00541  O-Antigen nucleotide sugar biosynthesis
ec01100  Metabolic pathways
Orthology
K21130  GDP-L-colitose synthase
Genes
YSI: BF17_13440
AAPC: QG404_01575
VGI: MID13_00930
VOX: LCH97_15630
PANX: OYT13_23730
SNOS: K8P63_01935
PAUN: MJA45_22845
MSIL: METEAL_02500(fcl)
CFAS: Cfast33896_17320(rfbD_3)
FBQ: D1817_12925
Reference
1  [PMID:15610039]
  Authors
Alam J, Beyer N, Liu HW
  Title
Biosynthesis of colitose: expression, purification, and mechanistic characterization of GDP-4-keto-6-deoxy-D-mannose-3-dehydrase (ColD) and GDP-L-colitose synthase (ColC).
  Journal
Biochemistry 43:16450-60 (2004)
DOI:10.1021/bi0483763
  Sequence
[ag:AEP25495]
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.356
IUBMB Enzyme Nomenclature: 1.1.1.356
ExPASy - ENZYME nomenclature database: 1.1.1.356
BRENDA, the Enzyme Database: 1.1.1.356
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