KEGG ENZYME: 1.10.3.15

ENZYME: 1.10.3.15

Entry

EC 1.10.3.15                Enzyme

Name

grixazone synthase;
GriF

Class

Oxidoreductases;
Acting on diphenols and related substances as donors;
With oxygen as acceptor

Sysname

3-amino-4-hydroxybenzoate:N-acetyl-L-cysteine:oxygen oxidoreductase

Reaction(IUBMB)

2 3-amino-4-hydroxybenzoate + N-acetyl-L-cysteine + 2 O2 = grixazone B + 4 H2O + CO2 [RN:R10720]

Reaction(KEGG)

R10720

Substrate

3-amino-4-hydroxybenzoate [CPD:C12115];
N-acetyl-L-cysteine [CPD:C06809];
O2 [CPD:C00007]

Product

grixazone B [CPD:C20799];
H2O [CPD:C00001];
CO2 [CPD:C00011]

Comment

A type 3 multi copper protein. The enzyme, isolated from the bacterium Streptomyces griseus, catalyses an 8 electron oxidation. Activation of the enzyme requires a copper chaperone (GriE). It also acts on 3-amino-4-hydroxybenzaldehyde, giving grixazone A. The second aldehyde group is presumably lost as formate. The enzyme also catalyses the reaction of EC 1.10.3.4 o-aminophenol oxidase.

History

EC 1.10.3.15 created 2014

Pathway

ec00997

Biosynthesis of various other secondary metabolites

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K20204

grixazone synthase / o-aminophenol oxidase