KEGG   ENZYME: 1.14.11.55
Entry
EC 1.14.11.55               Enzyme                                 
Name
ectoine hydroxylase;
ectD (gene name);
ectoine dioxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
Sysname
ectoine,2-oxoglutarate:oxygen oxidoreductase (5-hydroxylating)
Reaction(IUBMB)
ectoine + 2-oxoglutarate + O2 = 5-hydroxyectoine + succinate + CO2 [RN:R08050]
Reaction(KEGG)
R08050
Substrate
ectoine [CPD:C06231];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
5-hydroxyectoine [CPD:C16432];
succinate [CPD:C00042];
CO2 [CPD:C00011]
Comment
Requires Fe2+ and ascorbate. The enzyme, found in bacteria, is specific for ectoine.
History
EC 1.14.11.55 created 2017
Pathway
ec00260  Glycine, serine and threonine metabolism
ec01100  Metabolic pathways
Orthology
K10674  ectoine hydroxylase
Genes
SALYE8E00_13000
SKSFCN78_00650
SCOTHBA18_00655
PSAPST_0178
PSZPSTAB_0243
PSRPSTAA_0241
PSCA458_20390
PSJPSJM300_01235
PSHPsest_4093
PSTUUIB01_20905
PSTTCH92_00965
PCHLLLJ08_19550(thpD)
PBMCL52_19825
PSEDDM292_17915(thpD)
PNNKEM63_04675(thpD)
HPEGEAO82_11175(thpD)
MAQMaqu_1849 Maqu_3892
MHCMARHY1452(ectD) MARHY3849(ectD)
MADHP15_3697
MSRAU15_11815
MSXAU14_15005
MARIACP86_09080
MLQASQ50_10860
AHLAHTJS_13325
HMIsoil367_13195(thpD) soil367_14180(thpD)
CELLCBR65_07935
SPOIIMCC21906_00102
SNANI6N98_03395(thpD)
ZALAZF00_16385
MCELLPW13_06595
KIMG3T16_12870
LIBE4T55_13830
LSSNCTC12082_02698
MAHMEALZ_3246(ectD)
CYQQ91_0890
CZACYCME_1712
CYYCPC19_08285(thpD)
NOCNoc_1560
NHLNhal_2706
NWANwat_1573
NWRE3U44_10635(thpD)
AEHMlg_0392
HCHHCH_01507
HAHEENC22_23015(thpD)
CSACsal_0542 Csal_3003
HELHELO_4008(ectD)
HCSFF32_02870
HAKKO116_00988
HAMHALO0171 HALO0980
HHUAR456_13315
HCOLOKO_00567 LOKO_03086
HSIBOX17_06195
HALOBWR19_12730 BWR19_17025
HHHCLM76_02840(thpD)
HBEBEI_0262(ectD1) BEI_2549(ectD2)
HAGBB497_06365
HAFC8233_10080(thpD)
HALKCUU95_09235(thpD)
HVNEI420_16905(thpD)
HOLHORIV_56850 HORIV_69520
HSRHSBAA_64280
HMDCTT34_15390(thpD)
HAXIHAALTHF_18370n(ectD)
HTTHZS52_05815(thpD) HZS52_09840(thpD)
HCAMI4484_14265(thpD) I4484_19325(thpD)
HPIZGYM47_14595(thpD)
HSXHNO51_14005(thpD) HNO51_19405(thpD)
HSVHNO53_14665(thpD) HNO53_19665(thpD)
HTXEKK97_15830(thpD) EKK97_21770(thpD)
CMAIBFX80_16135
CAMHLCW13_01160(thpD)
KUSB9G99_08515
KMAB9H00_06335
KUYFY550_02245(thpD)
ABOABO_0023
ADIB5T_04304(ectD)
APACS7S_04430
ALNAS19_00240
AXEP40_20965
MMWMmwyl1_1161
MPRIMP3633_2842(ectD)
MARDIBG28_04615(thpD)
MARSA8C75_01140
NCUF0U83_00805(thpD)
NJPNEJAP_1907(ectD)
GSNYC6258_03085
OCMCBP12_09500
SALNSALB1_2995
CHRBDK843_11415(thpD)
CHRMFYK34_14440(thpD)
PTXABW99_08495
LIMNHKT17_04675
BPARBN117_2970
BPABPP1891
BBHBN112_1019
BBRBB3217
BBMBN115_1934
BBXBBS798_3049
BPTBpet1984
BAVBAV2371(thpD)
BHOD560_0723(thpD)
BHMD558_0712(thpD)
BHZACR54_03468
BTRMSAMEA390648704056(thpD_2)
BBROBAU06_08670
BFZBAU07_17490
BPDZBBN53_05230
BOHAKI39_08445
BGMCAL15_10345
BOJCBF45_15570
AXYAXYL_02147(thpD)
AXONH44784_037291
AXNAX27061_1961
AXXERS451415_01957
ADTAPT56_09400
AISBUW96_01105
ASWCVS48_14530(thpD)
ACHRC2U31_25480(thpD)
ACHBDVB37_08455(thpD)
ADYHLG70_12720(thpD)
PUTPT7_1540
PUSCKA81_07515(thpD)
PUDG9Q38_12440(thpD)
AKATKWG_13545
AMIMMIM_c17790(ectD2)
CDNBN940_07421
AFAUZ73_02845
AFQAFA_12625(thpD)
AAQUD3M96_13255(thpD)
AAMMFE795_11605(thpD)
ODIODI_R1925
BOZDBV39_06675(thpD)
PIGEGT29_07190(thpD)
KGYEHF36_15620(thpD)
PARKLSG25_01030(thpD)
CSERCCO03_13305
HYLLPB072_16115
HYCE5678_08805(thpD)
HYNF9K07_04935(thpD)
HARHEAR3377(ectD)
MMSmma_3598
ASKEI285_00510
AELLAELL_2036(ectD)
AAQIAAQM_0635(ectD)
ACLOACLO_1891(ectD)
AVPAVENP_2280(ectD)
ADZADFLV_2193(ectD)
AHSAHALO_1556(ectD)
AANAAANAER_1799(ectD)
ARCABLL_2145
AAKAA2016_5701
NKIKW403_06160(thpD)
PMOBHG718_03635(thpD)
ABAWD5400_10860(thpD)
PHLKKY_3694
DEQXM25_02185
DEIC4375_19440(thpD)
DSALK1X15_04305(thpD)
MMYRMXMO3_01132
YTIFNA67_05985(thpD)
AUBLXB15_00010(thpD)
RBMTEF_18675
PZUPHZ_c1338
TPROGa0080559_TMP4044
SUAMBOO69_06190
RIDRIdsm_03265
ROHFIU89_12560
SAGUCDO87_20350(thpD)
OCDFHY55_00400(thpD)
TMDKUV46_07470(thpD)
PALXGQA70_07695(thpD)
LITFPZ52_06940(thpD)
HNEHNE_1642
NPPPP1Y_AT4611
NPNJI59_09550
NREBES08_28565
NOVTQ38_019225(thpD)
SALSala_2952
SPHKSKP52_15805(ectD)
SPHPLH20_15525
SMAGAN936_05685
SMAZLH19_20385
STERAOA14_07660
SGISGRAN_2348(ectD)
SPHOC3E99_07240(thpD)
SPHXE5675_00345(thpD)
SPHUSPPYR_3379(ectD)
SPHITS85_04680
SSAUH8M03_00155
SSUAFPZ54_02065(thpD)
SSYSLG_08070(ectD)
SYBTZ53_24890
SBDATN00_18615
SPMIK663_18946
SPHRBSY17_1172(thpD)
SPHTK426_26465
SHYDCJD35_04525(thpD)
SYAA6768_16850(thpD)
SCLOSCLO_1025180
SPPHKFK14_00900(thpD)
SPHJBSL82_01415
SMICSmB9_21720
ALBAEB_P3387
ACRAcry_3011
AMVACMV_33550(ectD)
NCBC0V82_13055(thpD)
TXITH3_05475
THACCSC3H3_05330(thpD)
BSHILGQ02_01520(thpD)
BCLABC3489
BPFBpOF4_12395(ectD)
HHDHBHAL_3406(ectD)
HMNHM131_04820
VIRX953_17730
VHLBME96_17215
VIMGWK91_05190(thpD)
LAOAOX59_07340
SJEAAV35_000930
GRCGI584_06005(thpD)
RUEDT065_11560(thpD)
SALEEPH95_10530(thpD) EPH95_17035(thpD)
SCIBHUG20_00440(thpD) HUG20_05415(thpD)
SCIAHUG15_00695(thpD) HUG15_07680(thpD)
SEDDERJ70_02585(thpD)
HYIK2M58_09915(thpD)
BCHSJNE38_24520(thpD)
GYMGYMC10_5662
PNPIJ22_35870
POWIJ21_09520
PVOPVOR_30643
PLWD5F53_01670(thpD)
PLYCGXP70_14615(thpD)
AFXJZ786_02055(thpD)
ACURJZ785_17555(thpD)
EFFskT53_16880
PABSJIR001_28580
MMCMmcs_4193
MKMMkms_4259
MJLMjls_4420
MYVG155_17680
MGOAFA91_20570
MDXBTO20_14020
MNMMNVM_33490(ectD)
MKRMKOR_19740(thpD)
MDFK0O62_14465(thpD)
MHOLK3U96_24235(thpD)
MHERK3U94_15445(thpD)
MSENK3U95_15080(thpD)
MVMMJO54_15400(thpD)
MCROMI149_06355(thpD)
MSMMSMEG_3898(thpD)
MSGMSMEI_3808
MSBLJ00_19365
MSNLI99_19370
MSHLI98_19375
MVAMvan_5271
MGIMflv_4835
MSPMspyr1_42450
MCBMycch_2331
MNED174_14290
MYNMyAD_14035
MFTXA26_36040
MPHLMPHLCCUG_01412
MTHN4412656_01027
MHASMHAS_00348(ectD)
MDUMDUV_49230(thpD)
MCHTMCHIJ_24920(thpD)
MAICMAIC_44760(thpD)
MALVMALV_03900(thpD)
MTYMTOK_05960(thpD)
MGADMGAD_30340(thpD)
MPOFMPOR_17990(thpD)
MMATMMAGJ_70990(thpD)
MBOKMBOE_38050(thpD)
MFGK6L26_14255(thpD)
MFLVNCTC10271_01042
MCEEMCEL_43410(thpD)
MABMAB_2264c
MMVMYCMA_1216
MABBMASS_2187
MABLMMASJCM_2212
MCHEBB28_11340
MIZBAB75_12095
MSTEMSTE_02193
MSAOMYCSP_09710
MSALDSM43276_01967
MJDJDM601_2700
MTER4434518_02648
MMINMMIN_04010(ectD)
MHIBMHIB_21320(ectD)
ASDAS9A_2077(ectD)
MPAKMIU77_13740(thpD)
NFANFA_27190(ectD)
NFRERS450000_01378
NCYNOCYR_3186(ectD)
NBRO3I_025725
NNONONO_c56240(ectD)
NSLBOX37_12430
NSRNS506_03741(ectD)
NTPCRH09_08825(thpD) CRH09_28080(thpD)
NOZDMB37_05120(thpD)
NODFOH10_10690(thpD)
NAHF5544_23680(thpD)
NADNCTC11293_04314
NWLNWFMUON74_21420(ectD)
NIEKV110_25420(thpD)
NTCKHQ06_19525(thpD)
NHUH0264_22730(thpD)
NYALTV02_16825(thpD)
NGPLTT66_28280(thpD)
RHARHA1_ro04580 RHA1_ro04934
REBXU06_31605
ROPROP_44930(ectD) ROP_50010(ectD)
ROAPd630_LPD01050 Pd630_LPD01448
REQREQ_07850
RPYY013_20325
RBYCEJ39_06835(thpD)
RAVAAT18_05970
RHWBFN03_13990
RRZCS378_21165(thpD)
RHQIM25_10150
RHODAOT96_31670
RRT4535765_00875
RCRNCTC10994_01835
RTMG4H71_06210(thpD)
RKOJWS14_24980(thpD) JWS14_27240(thpD)
RGOKYT97_12215
GORKTR9_4109
GOQACH46_01240
GTABCM27_21705
GOCCXX93_02050(thpD)
GITC6V83_11400(thpD)
GRUGCWB2_20775
GOMD7316_04338(ectD)
GAVC5O27_07265
GODGKZ92_19975
GAMGII34_03370(thpD)
GOILK459_12770(thpD)
GHNMVF96_20775(thpD)
GAMIIHQ52_00545
TPRTpau_3672
TSMASU32_20955
DTMBJL86_2147 BJL86_2615
DITC3V38_09130(thpD)
DIZCT688_03045
DLUA6035_03285
TOYFO059_13125(thpD)
SCOSCO1867(SCI39.14)
SALBXNR_4959
SMASAVERM_6395(ectD)
SGRSGR_5632(ectD)
SGBWQO_07155
SCBSCAB_70711(ectD)
SSXSACTE_1287
SVLStrvi_6959
SHYSHJG_3328 SHJG_8671
SHOSHJGH_3093 SHJGH_8431
SRCM271_36930
SMALSMALA_1826
SSOII1A49_12280(thpD)
SCTSCAT_1067(ectD)
SCYSCATT_10620
SFASfla_4955 Sfla_5672
SBHSBI_08130(ectD)
SVESVEN_0229
SDVBN159_6648(ectD)
SALSSLNWT_6006
STRPF750_0924 F750_1726
SFISFUL_1437
SCIB446_09715
SALUDC74_2369
SALLSAZ_13200
SLVSLIV_28380(ectD)
SGUSGLAU_08340(ectD)
SVTSVTN_02540
STREGZL_06637
SCWTU94_03765 TU94_07930
SLDT261_6279
SLCSL103_09830
SXISXIM_08370
STRCAA958_05315
SAMBSAM23877_1941(ectD)
SPRISPRI_5647
SCZABE83_28645
SCXAS200_33875
SRWTUE45_01704 TUE45_02348 TUE45_03036
STRFASR50_09085
SLEsle_52670(sle_52670)
SRNA4G23_01095
SPAVSpa2297_07450
STRTA8713_06900
SCLFBB341_22625
SGSAVL59_36165
STSIA4E84_09420 A4E84_36765
SKYD0C37_26805(thpD)
SALWCP975_07895(thpD)
SGMGCM10017557_63920(ectD)
SCADDN051_29180(thpD)
SLSSLINC_2135
SNRSNOUR_30890(ectD)
SPLULK06_006325
STRDNI25_29875
SNWBBN63_27150
SAUOBV401_12600
SSIAA7J05_27590
SVUB1H20_06325
SPUNBFF78_31950
SGVB1H19_11430
SLAUSLA_7411
SALFSMD44_02016(ectD)
SALJSMD11_5132(ectD) SMD11_6819(ectD)
STROSTRMOE7_10265
SFKKY5_1780
SNZDC008_07570(thpD)
SGEDWG14_06535(ectD)
SRJSRO_5592
SLKSLUN_09020(thpD)
SDXC4B68_30980(thpD)
SGDELQ87_00200(thpD) ELQ87_30210(thpD)
SQZFQU76_06110(thpD)
SCYAEJ357_10705(thpD)
SASTCD934_26320(thpD)
SNQCP978_09005(thpD)
STIRDDW44_02720(thpD)
SKACP970_33760(thpD)
SGZC0216_09925(thpD)
SNKCP967_27015(thpD)
SHAWCEB94_09875(thpD)
SRKFGW37_07925(thpD)
SFICEIZ62_25770(thpD)
SGALCP966_07990(thpD)
SSPODDQ41_02140(thpD)
SFYGFH48_30770(thpD)
SAQUEJC51_12480(thpD)
SGFHEP81_05897(thpD)
SCAVCVT27_06035(thpD)
SSEOD0Z67_06295(thpD)
SBYH7H31_27965(thpD)
SRIMCP984_31635(thpD)
SSUBCP968_31760(thpD)
SPHVF9278_09355(thpD)
SCINCP977_02885(thpD)
SFUGCNQ36_08070(thpD)
SPACB1H29_28150
STSUB7R87_06095
SCHACP983_33390(thpD)
SGXH4W23_03090(thpD)
SCOECP976_10605(thpD)
SBATG4Z16_27210(thpD)
SSPBCP982_11010(thpD)
SPLACP981_09360(thpD)
SBROGQF42_12190(thpD)
STUIGCM10017668_14640(ectD)
SATAC5746_10230(thpD)
SCHFIPT68_08640(thpD)
SHUNDWB77_05853(ectD)
SHARHUT13_04745(thpD)
SCYGS1361_10250
SFEUIM697_12920(thpD)
SDWK7C20_08875(thpD)
SVDCP969_10380(thpD)
SCALI6J39_06495(thpD)
SAUHSU9_007375(thpD)
SFBCP974_05675(thpD)
SPRACP972_08460(thpD)
SPEUCGZ69_08005(thpD)
SROIIAG44_03305(thpD)
SXNIAG42_27490(thpD)
SGJIAG43_06855(thpD)
SHKJ2N69_07715(thpD) J2N69_33620(thpD)
SANUK7396_27460(thpD)
STRYEQG64_06120(thpD)
SDDD9753_27415(thpD)
SACTDMT42_08570(thpD)
SMAOCAG99_23570
SGOBtest1122_02425(thpD)
SINEKI385_11970(thpD)
SLFJEQ17_35600(thpD)
SDECL3078_10850(thpD)
KABB7C62_06140
YIALO772_19070(thpD)
MCHNHCR76_00590(thpD)
SALDFVA74_05325(thpD)
ACRYAC20117_07935
AULDCC27_009910(thpD)
BFABfae_05540
BRXBH708_16535
KSEKsed_19670
LMOIVV02_20580
BLINBLSMQ_1388
HALTIM660_06890(thpD)
NPCKUV85_10685(thpD)
THAONI17_022010(thpD)
TALXFOF52_19195(thpD)
NDANdas_0138
NALB005_2939(thpD)
NGVCDO52_05680(thpD)
STRREKD16_02615
ACTWF7P10_37530(thpD)
AGRAAGRA3207_003665(thpD) AGRA3207_006984(thpD)
SENSACE_0486(ectD)
SACGFDZ84_14085(thpD)
SVISvir_34780
SACCEYD13_19290
AMDAMED_8594(ectD)
AMNRAM_44110
AMMAMES_8463(ectD)
AMZB737_8464(ectD)
AOIAORI_7380(ectD)
AJAAJAP_02100(ectD)
AMQAMETH_6462(ectD)
AMYCCU254_35655(thpD)
AMYBBKN51_30305
AABA4R43_26270
AMYYYIM_03165
AORISD37_03570
AACDLWP59_35945(thpD)
PDXPsed_5823
PSEAWY02_14010
PSEEFRP1_28540
PSEQAD006_07715
PECQAD017_15530
PHHAFB00_13365
PAUTPdca_62800(ectD)
PBROHOP40_10395(thpD)
KALKALB_3094
KPHYAOZ06_02525
AHMTL08_02005
ACTIUA75_01850
ACADUA74_01845
AHGAHOG_01800
ACTAC1701_09155(thpD)
PMADBAY61_17755 BAY61_28350
SACEGIY23_02055(thpD)
ATLAthai_19340(ectD)
ASERAsera_55210(ectD)
SNASnas_5814
NAVJQS30_12445(thpD) JQS30_12450(thpD)
GLYK3N28_20635(thpD)
AEYCDG81_13645(thpD)
ATQGH723_16280(thpD)
BVOPan97_41970
PBSPlabr_3303
GMRGmarT_46250
GIMF1728_22025(thpD)
SDYNMal52_58560
SLRL21SP2_2138
LFILFML04_0402
LFPY981_02060
LEGABH19_02130
NLIG3M70_03590(thpD)
NMRNmar_1343
NIRNSED_02010
NKRNKOR_06015
NIPNsoK4_06430(thpD)
 » show all
Reference
1  [PMID:17636255]
  Authors
Bursy J, Pierik AJ, Pica N, Bremer E.
  Title
Osmotically induced synthesis of the compatible solute hydroxyectoine is mediated by an evolutionarily conserved ectoine hydroxylase.
  Journal
J Biol Chem 282:31147-55 (2007)
DOI:10.1074/jbc.M704023200
  Sequence
Reference
2  [PMID:18849444]
  Authors
Bursy J, Kuhlmann AU, Pittelkow M, Hartmann H, Jebbar M, Pierik AJ, Bremer E
  Title
Synthesis and uptake of the compatible solutes ectoine and 5-hydroxyectoine by Streptomyces coelicolor A3(2) in response to salt and heat stresses.
  Journal
Appl Environ Microbiol 74:7286-96 (2008)
DOI:10.1128/AEM.00768-08
  Sequence
[sco:SCO1867]
Reference
3  [PMID:20498719]
  Authors
Reuter K, Pittelkow M, Bursy J, Heine A, Craan T, Bremer E
  Title
Synthesis of 5-hydroxyectoine from ectoine: crystal structure of the non-heme iron(II) and 2-oxoglutarate-dependent dioxygenase EctD.
  Journal
PLoS One 5:e10647 (2010)
DOI:10.1371/journal.pone.0010647
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.11.55
IUBMB Enzyme Nomenclature: 1.14.11.55
ExPASy - ENZYME nomenclature database: 1.14.11.55
BRENDA, the Enzyme Database: 1.14.11.55
LinkDB

DBGET integrated database retrieval system