KEGG   ENZYME: 1.14.11.57
Entry
EC 1.14.11.57               Enzyme                                 

Name
L-proline trans-4-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
Sysname
L-proline,2-oxoglutarate:oxygen oxidoreductase (trans-4-hydroxylating)
Reaction(IUBMB)
L-proline + 2-oxoglutarate + O2 = trans-4-hydroxy-L-proline + succinate + CO2 [RN:R01252]
Reaction(KEGG)
R01252
Substrate
L-proline [CPD:C00148];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
trans-4-hydroxy-L-proline [CPD:C01157];
succinate [CPD:C00042];
CO2 [CPD:C00011]
Comment
Requires Fe2+ and ascorbate. The enzyme, isolated from multiple bacterial species, only produces trans-4-hydroxy-L-proline (cf. EC 1.14.11.56, L-proline cis-4-hydroxylase).
History
EC 1.14.11.57 created 2017
Orthology
K21615  L-proline 4-hydroxylase
Genes
PAO: Pat9b_4264
PLZ: S4054249_22495
PSEN: PNC201_00730
AMAA: amad1_21538
AMAL: I607_20252
AMAE: I876_01340
AMAO: I634_01440
AMAI: I635_21544
BSTG: WT74_26330
BGM: CAL15_21815
AMIM: MIM_c00990(ectD1)
ODI: ODI_R4011
POL: Bpro_1714
PVAC: HC248_03375(ectD)
RTA: Rta_12710
BJA: blr7814(blr7814)
BRA: BRADO6432
BBT: BBta_1202
BRS: S23_63740
AOL: S58_65510
BRAD: BF49_3241
BARH: WN72_43850
RBM: TEF_17475
SMAZ: LH19_18060
BLAS: BSY18_3438
ACR: Acry_1558
SHUM: STHU_48060
TXI: TH3_14860
SFA: Sfla_0103
SBH: SBI_06435
STRP: F750_6922
SLD: T261_7676
SFK: KY5_3455
SAQ: Sare_4561
AVA: Ava_C0003
DOU: BMF77_01760(ectD)
CPI: Cpin_5186
 » show all
Reference
1  [PMID:8546682]
  Authors
Lawrence CC, Sobey WJ, Field RA, Baldwin JE, Schofield CJ
  Title
Purification and initial characterization of proline 4-hydroxylase from Streptomyces griseoviridus P8648: a 2-oxoacid, ferrous-dependent dioxygenase involved in etamycin biosynthesis.
  Journal
Biochem J 313 ( Pt 1):185-91 (1996)
DOI:10.1042/bj3130185
Reference
2  [PMID:10473412]
  Authors
Shibasaki T, Mori H, Chiba S, Ozaki A
  Title
Microbial proline 4-hydroxylase screening and gene cloning.
  Journal
Appl Environ Microbiol 65:4028-31 (1999)
DOI:10.1128/AEM.65.9.4028-4031.1999
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.11.57
IUBMB Enzyme Nomenclature: 1.14.11.57
ExPASy - ENZYME nomenclature database: 1.14.11.57
BRENDA, the Enzyme Database: 1.14.11.57
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