KEGG   ENZYME: 1.14.11.60
Entry
EC 1.14.11.60               Enzyme                                 
Name
scopoletin 8-hydroxylase;
S8H (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
Sysname
scopoletin,2-oxoglutarate:oxygen oxidoreductase (8-hydroxylating)
Reaction(IUBMB)
scopoletin + 2-oxoglutarate + O2 = fraxetin + succinate + CO2 [RN:R12222]
Reaction(KEGG)
R12222
Substrate
scopoletin [CPD:C01752];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
fraxetin [CPD:C09265];
succinate [CPD:C00042];
CO2 [CPD:C00011]
Comment
Requires iron(II) and ascorbate. A protein involved in biosynthesis of iron(III)-chelating coumarins in higher plants.
History
EC 1.14.11.60 created 2018
Pathway
ec00999  Biosynthesis of various plant secondary metabolites
ec01110  Biosynthesis of secondary metabolites
Orthology
K23145  scopoletin 8-hydroxylase
Genes
ATH: AT3G12900
ALY: 9318860
CRB: 17892971
CSAT: 104745510 104748679 104765057 104777120
EUS: EUTSA_v10020841mg
BRP: 103849720 103859475 103870212
BNA: 106345421 106375087 106375542 106382146 106386876 106400351
BOE: 106293989 106301500 106333669
RSZ: 108810911 108835958 108861046
THJ: 104810262
CPAP: 110809857
CIT: 102607640
CIC: CICLE_v10006882mg
PVY: 116119197 116133563
MINC: 123206691 123226450
TCC: 18594550 18594551 18594553 18594556
GRA: 105761379 105775787 105775819 105784310 105784311 105787151 105787154
GHI: 107886197 107911338 107912932 107913465 107919665 107932640 107932661 107932662 107936166 107936167 107936168 107936170 121223450
GAB: 108452509 108461393 108461394 108480494 108480495 108480496 108480497 108482454 108482635
HSYR: 120123825 120127654 120127655 120127656 120129162 120155394 120155446 120161610
DZI: 111276015 111276016 111276018 111276020 111276597 111276599 111276600 111287890 111318382 111318388 111318392
GMX: 100803722 102659647
GSJ: 114423069 114423906
PVU: 137835086 137836233
VRA: 106761811
VAR: 108333781
VUN: 114181474
VUM: 124832952
CCAJ: 109790572 109790573 109790576 109818815
MTR: 11407206 11409249
TPRA: 123898291
CAM: 101492926
PSAT: 127128436
VVO: 131596845 131596847 131598917 131598918 131598919 131598922 131598923 131598924 131598926 131634121 131634122 131634123 131634124 131634125 131634128
LJA: 130743703 130745530
ADU: 107466129 107471942 107480135
AIP: 107619811 107630421
AHF: 112708410 112711180 112738254 112779478 112791023
LANG: 109333837 109355985
FVE: 105350415
AANS: 126788467
RCN: 112203388
PPER: 18778908
PMUM: 103325695
PAVI: 110768994
PDUL: 117626205
MDM: 103435419
MSYL: 126623735
PXB: 103952012
PCOX: 137724436
MNT: 21399318
CSAV: 115709070
RCU: 107260838 8272615
MEAN: 126655283 126656567 126656636 126671528 126671529 126671535 126679851 126681880
JCU: 105646195 105646196
HBR: 110638461 110662018 110662019 110662020
MESC: 110617017 110630845 122721679 122723798
POP: 7480442
PEU: 105123997
PALZ: 118054079
PNZ: 133693505
JRE: 109007025
CILL: 122311404
CAVE: 132174257
AGLU: 133876126 133879442
QSU: 111986809 112009979
QLO: 115984049 115984050
TWL: 120009221
VVI: 100259503 100854658 104877301
VRI: 117908689 117908690 117910864
SLY: 101262174
SPEN: 107004094
SOT: 102584589
SSTN: 125844705
SDUL: 129903706 129904688
LBB: 132644063
NTA: 107766774 107821681
NSY: 104226661
NTO: 104118671
NAU: 109238378
INI: 109160293
ITR: 116005047
OEU: 111396786
HAN: 110878125 110878126 110878127 110937900 110939629 110939645 110939654 110939655
ECAD: 122594033
LSV: 111890983 111890990 111891065 111902368
CCAV: 112522925
DCR: 108216893 108218127
CSIN: 114267034
RVL: 131321855
AEW: 130754918 130761620 130773728
NNU: 104603880 104609462
MING: 122059183 122063766 122063776 122067316 122092711
TSS: 122640930 122649187
PSOM: 113289572 113298261 113298269 113326671 113332375
MSIN: 131243293 131243300
ACAK: 137943763
ATR: 18424098 18424099 18424100 18424103
 » show all
Reference
1  [PMID:29361149]
  Authors
Siwinska J, Siatkowska K, Olry A, Grosjean J, Hehn A, Bourgaud F, Meharg AA, Carey M, Lojkowska E, Ihnatowicz A
  Title
Scopoletin 8-hydroxylase: a novel enzyme involved in coumarin biosynthesis and iron-deficiency responses in Arabidopsis.
  Journal
J Exp Bot 69:1735-1748 (2018)
DOI:10.1093/jxb/ery005
  Sequence
[ath:AT3G12900]
Reference
2  [PMID:29581584]
  Authors
Rajniak J, Giehl RFH, Chang E, Murgia I, von Wiren N, Sattely ES
  Title
Biosynthesis of redox-active metabolites in response to iron deficiency in plants.
  Journal
Nat Chem Biol 14:442-450 (2018)
DOI:10.1038/s41589-018-0019-2
  Sequence
[ath:AT3G12900]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.11.60
IUBMB Enzyme Nomenclature: 1.14.11.60
ExPASy - ENZYME nomenclature database: 1.14.11.60
BRENDA, the Enzyme Database: 1.14.11.60
LinkDB

DBGET integrated database retrieval system