KEGG ENZYME: 1.14.12.7

ENZYME: 1.14.12.7

Entry

EC 1.14.12.7                Enzyme

Name

phthalate 4,5-dioxygenase;
PDO ;
phthalate dioxygenase

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor

Sysname

phthalate,NADH:oxygen oxidoreductase (4,5-hydroxylating)

Reaction(IUBMB)

phthalate + NADH + H+ + O2 = cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,2-dicarboxylate + NAD+ [RN:R03630]

Reaction(KEGG)

R03630

Substrate

phthalate [CPD:C01606];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]

Product

cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,2-dicarboxylate [CPD:C04783];
NAD+ [CPD:C00003]

Comment

A system, containing a reductase which is an iron-sulfur flavoprotein (FMN), an iron-sulfur oxygenase, and no independent ferredoxin. Requires Fe2+.

History

EC 1.14.12.7 created 1990

Pathway

ec00624

Polycyclic aromatic hydrocarbon degradation

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K18068

phthalate 4,5-dioxygenase

Genes

PLUL:

FOB45_02790

PHEF:

PSH57_18305

PSSX:

DCC84_11960

PAGB:

AWM79_07100 AWM79_17680

MBS:	MRBBS_1311(cbaA)

HARO:

R50071_44690

HAM:

HALO0380

HANY:

LG409_17180

HSPE:

IT895_17425

HSR:	HSBAA_48220

HMD:	CTT34_16730

VRS:	V8F66_15330 V8F66_21150

HSX:	HNO51_14950

HAMC:

HNO52_05225

MPRF:

J8N69_04550

MGEO:

CFI10_14950

GBI:	PG2T_14045

RPF:	Rpic12D_2779 Rpic12D_4958

CNC:	CNE_2c21230(pht1)

CUH:	BJN34_28850

REU:	Reut_B4795 Reut_B5781 Reut_C6324

RME:

Rmet_5548

CBW:	RR42_s1578

CCUP:

BKK81_29815

CUP:	BKK80_26540

CUU:	BKK79_32425

COX:	E0W60_20460

CUK:	KB879_16005

BVI:	Bcep1808_4273

BCEN:

DM39_1999

BMJ:	BMULJ_03712(ophA2)

BMU:

Bmul_4805

BMK:

DM80_4020

BUB:	BW23_5728 BW23_5729

BYI:	BYI23_B001650 BYI23_C002440 BYI23_D008300

BPLA:

bpln_1p1370

BURT:

BTHE68_67770

BXE:	Bxe_A3529 Bxe_A3537 Bxe_B2370

BXB:	DR64_1227 DR64_1235 DR64_4702

BPH:	Bphy_4881 Bphy_4882

BGE:	BC1002_3595 BC1002_4693

BPY:	Bphyt_7254

BUZ:	AYM40_12125 AYM40_32995 AYM40_33000

BCAI:

K788_0000443

PSPW:

BJG93_04590

PHS:	C2L64_49415

PTER:

C2L65_44680

PEW:	KZJ38_26705

PDIO:

PDMSB3_0711.1

PPAI:

E1956_41115 E1956_41120

PPUL:

RO07_06175

PSPU:

NA29_21445

POX:	MB84_08085

PFG:	AB870_18750

PNR:	AT302_05945 AT302_13310

PFIB:

PI93_011765 PI93_023915

PCOM:

NTU39_07750

PANN:

PanNE5_13500

PANX:

OYT13_00095

BUO:	BRPE64_DCDS03430 BRPE64_DCDS07110

CABK:

NK8_60880

BUE:	BRPE67_BCDS04040 BRPE67_CCDS09780 BRPE67_DCDS10220

BBH:	BN112_3649

BBR:

BB4773

BBM:	BN115_4450

BBX:	BBS798_4550

BPT:

Bpet0252

BHZ:	ACR54_04439(pht3)

BBRO:

BAU06_03620 BAU06_03750 BAU06_03780

BFZ:	BAU07_13845 BAU07_15835 BAU07_15865

BPDZ:

BBN53_00955

BOH:	AKI39_21300 AKI39_21330

BGM:	CAL15_17820

AAEG:

RA224_28120

AVH:	WHX56_15235

POLL:

OEG81_03545 OEG81_15670

PIG:	EGT29_10005 EGT29_10580 EGT29_22455 EGT29_23240 EGT29_25710 EGT29_27735 EGT29_27745

PARK:

LSG25_14025

PUD:	G9Q38_10670

RHY:	RD110_25740

RHOB:

HTY51_11680 HTY51_11745 HTY51_17795

POL:

Bpro_1910

PNA:

Pnap_4627

POS:	DT070_20425

POO:	F7R28_10150

ACID:

CBP33_05530

ACIP:

CBP36_05980 CBP36_20410

ACIN:

CBP34_05365

ACIS:

CBP35_12965 CBP35_20490

VEI:

Veis_2928

DEL:	DelCs14_1655 DelCs14_1661

DLA:	I6G47_32030 I6G47_32085 I6G47_32165 I6G47_32230 I6G47_33695

VPD:	VAPA_2c09710

VBO:	CKY39_21970

VAM:	C4F17_30665

CTT:	CtCNB1_1488

CTES:

O987_16325 O987_21865 O987_28200

CENP:

M9799_18560

OTK:	C6570_10585

LIM:	L103DPR2_00195(pht3_1) L103DPR2_00198(pht3_2) L103DPR2_02573(pht3_3)

LIH:	L63ED372_03070(pht3_2)

LIMI:

LINBF2_03510

HYR:	BSY239_2699 BSY239_3345 BSY239_3465

HYB:	Q5W_00860 Q5W_24655

HYN:	F9K07_00645 F9K07_00650 F9K07_00665 F9K07_06620 F9K07_07515 F9K07_11625 F9K07_11660 F9K07_11665 F9K07_11680 F9K07_22870 F9K07_27550 F9K07_30150 F9K07_30200 F9K07_30250 F9K07_30395 F9K07_30445

HTN:	KI616_03525 KI616_03615

HCZ:	G9Q37_00960 G9Q37_13970

HYY:	JNX00_11215

HYDA:

ABSY19_17145 ABSY19_21525

DIM:	K2L43_12460

SMIO:

CATMQ487_34500

MASW:

AM586_27410 AM586_27625 AM586_27690

THK:	CCZ27_15665

MESO:

BSQ44_11250

EMX:	FKV68_30120

STEG:

QA637_22935

SCHP:

RB548_27180

RHIW:

KQ933_32880 KQ933_33000

ABAW:

D5400_03680 D5400_20255

BJA:	blr0938(blr0938)

AOL:

S58_21510

BRAD:

BF49_7248

BIC:	LMTR13_01465

BRK:	CWS35_04530 CWS35_19800

BOT:	CIT37_09645

BGQ:	X265_35300

BVZ:	BRAD3257_6131

BEL:	BE61_11040 BE61_64940 BE61_87020

BDG:	LPJ38_20660

BSEP:

HAP48_0017415 HAP48_0032605

BQB:	J4P68_0020965 J4P68_0033840

BCAN:

BcanWSM471_36140

BAUT:

QA635_08390 QA635_40945

BBRA:

QA636_27065 QA636_42020

BGK:	IC762_02905 IC762_13670

BCOU:

IC761_35325

BPAH:

QA639_17945 QA639_41100 QA639_41225

BROS:

QUH67_02935

BRAY:

ML401_33380

BRAA:

NL528_44150

BLUP:

AB3L03_16240

BGEP:

ACP2YO_47295

BRAN:

RX330_35260

BRAB:

RX331_37260

RHOL:

RBJ75_26350

RHZ:	RHPLAN_00120 RHPLAN_01370 RHPLAN_59330

BVV:	BHK69_01760

BVES:

QO058_19460

BOI:	BLM15_16160

XDI:	EZH22_10365

PTAW:

DW352_11865 DW352_18640

MLG:	CWB41_10140

PNF:	V6617_14700

DSAL:

K1X15_01480

DEVD:

GHV40_19155

DPV:	ACE4RL_10940

HDI:	HDIA_4250(pht3)

MMED:

Mame_05096(pht3)

PSIN:

CAK95_02265 CAK95_05895

RBM:

TEF_13150

LABT:

FIU93_07195(pht1)

PHAE:

N1037_23055

CID:	P73_1053 P73_2875 P73_2968

CEH:	CEW89_13880

CBAK:

DA792_02400

CON:	TQ29_05745

TPRO:

Ga0080559_TMP5103

PABY:

Ga0080574_TMP2103

RID:	RIdsm_00905(pht3)

ROT:	FIV09_12260(pht5)

RMH:	LVO79_19250

MALU:

KU6B_35110

HDH:	G5B40_16605

LVR:	T8T21_19990

MANH:

LA6_006111(pht3_2)

NOR:	FA702_15515 FA702_19040

NDR:	HT578_08880

NOVB:

ACFFDA_03255 ACFFDA_11625 ACFFDA_11640

SPHI:

TS85_13270

SSAN:

NX02_22625

SBIN:

SBA_ch1_21310

SNAP:

PQ455_00695

SAAP:

E2E30_04350

SPCL:

F9288_01860

SPSO:

G4G27_23015 G4G27_23140

SPMS:

ACO227_17185 ACO227_17300

ACOB:

P0Y56_12150 P0Y56_16120

SPHP:

LH20_19475

RDI:	CMV14_06830 CMV14_06835

SSY:	SLG_07580 SLG_19120

SBD:	ATN00_07605

SPHR:

BSY17_2023 BSY17_4182

SHYD:

CJD35_19410 CJD35_19645

SPYG:

YGS_C1P1166

SPAG:

sphantq_03244(pht3_1) sphantq_04492(pht3_2)

SPCA:

GL174_20800 GL174_21630

SPJS:

NUH86_18185

SMIC:

SmB9_09290

AAY:	WYH_03341(pht3)

CMAN:

A9D14_14470

POT:	E2E27_18200

RMT:	IAI58_16250

TLU:	R1T41_00620

THGO:

M9H61_18410

THAD:

IT971_21445

MINW:

ACMVY4_18205

PALJ:

LZC94_30585

BFD:	NCTC4823_00630(kshA_1)

PERI:

RE409_28345 RE409_28410

SCIA:

HUG15_01335

NDT:	L1999_05680

NNA:	ACN6MT_23405

AIA:	AWH56_017995

PSAB:

PSAB_03650

PBJ:	VN24_18685

PNP:	IJ22_14360

ANEU:

PP175_12610 PP175_12630

AACO:

K1I37_05885 K1I37_05900

ADAU:

NZD86_13330 NZD86_15185 NZD86_15565 NZD86_15580

AFAS:

NZD89_07155 NZD89_12800 NZD89_17980

EFF:	skT53_12430 skT53_33890

SPOS:

DV702_11120

MGAU:

MGALJ_59780

MYW:	BVC93_29035

MRH:	MycrhN_3125

MGAD:

MGAD_32490

MGO:	AFA91_26505

MPAE:

K0O64_27755

MFRE:

EXE63_02915

NTP:	CRH09_08335

RKO:	JWS14_40240

RPSK:

JWS13_36735

RHOW:

A4U64_03195

RHOJ:

JVH1_38360

RHOS:

QWW67_11425

RHOQ:

QWW98_11435

RHJS:

OYT95_40220

RHZQ:

GO592_42140

RHPD:

U9J23_25590

RHWB:

FND50_32995

RHDM:

HFP48_30045

RHMM:

ISO16_11380

RHZP:

HYG77_33170

GOR:

KTR9_0709

GOQ:	ACH46_18515

SMAL:

SMALA_7807

SSOI:

I1A49_42875

STUI:

GCM10017668_58100

SSPN:

LXH13_02865

SLUO:

PS467_02515

STRL:

HEP84_46895

SRPZ:

HEP85_06545

ASUF:

MNQ99_18620

PUE:	FV140_21245

PHRZ:

QN084_10100

ARZ:	AUT26_02365 AUT26_19495

GMY:

XH9_09030

ACRY:

AC20117_22710

AFUV:

AADG42_01585

NPS:	KRR39_10780

NLB:	P5P86_01370

ACTQ:

OG417_26300

AACD:

LWP59_23325 LWP59_25695

ARHD:

VSH64_03930 VSH64_09950 VSH64_16450

ACAB:

QRX50_19585 QRX50_29820 QRX50_37835 QRX50_49170

ANW:	QP939_29135

AMYD:

K1T34_01090 K1T34_02405 K1T34_49805

PDX:	Psed_1414 Psed_3698 Psed_3921 Psed_3923

PSDS:

K1T35_19965 K1T35_28420

PMAD:

BAY61_17900

SAQ:

Sare_0574

PFLA:

Pflav_042940 Pflav_042960

PSUU:

Psuf_021450 Psuf_075920

DACT:

KZZ52_33355

RMAR:

GBA65_17255

LEPV:

J4558_17010(rpsC)

VAB:

WPS_26940

» show all

Reference

1 [PMID:3805038]

Authors

Batie CJ, LaHaie E, Ballou DP.

Title

Purification and characterization of phthalate oxygenase and phthalate oxygenase reductase from Pseudomonas cepacia.

Journal

J Biol Chem 262:1510-8 (1987)

Other DBs

ExplorEnz - The Enzyme Database:

1.14.12.7

IUBMB Enzyme Nomenclature:

1.14.12.7

ExPASy - ENZYME nomenclature database:

1.14.12.7

UM-BBD (Biocatalysis/Biodegradation Database):

1.14.12.7

BRENDA, the Enzyme Database:

1.14.12.7

CAS:	63626-44-8

LinkDB

DBGET integrated database retrieval system