KEGG ENZYME: 1.14.12.7

ENZYME: 1.14.12.7

Entry

EC 1.14.12.7                Enzyme

Name

phthalate 4,5-dioxygenase;
PDO ;
phthalate dioxygenase

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor

Sysname

phthalate,NADH:oxygen oxidoreductase (4,5-hydroxylating)

Reaction(IUBMB)

phthalate + NADH + H+ + O2 = cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,2-dicarboxylate + NAD+ [RN:R03630]

Reaction(KEGG)

R03630

Substrate

phthalate [CPD:C01606];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]

Product

cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,2-dicarboxylate [CPD:C04783];
NAD+ [CPD:C00003]

Comment

A system, containing a reductase which is an iron-sulfur flavoprotein (FMN), an iron-sulfur oxygenase, and no independent ferredoxin. Requires Fe2+.

History

EC 1.14.12.7 created 1990

Pathway

ec00624

Polycyclic aromatic hydrocarbon degradation

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K18068

phthalate 4,5-dioxygenase

Genes

PLUL:

FOB45_02790

MBS:	MRBBS_1311(cbaA)

HAM:

HALO0380

HSR:	HSBAA_48220

HMD:	CTT34_16730

HSX:	HNO51_14950

MPRF:

J8N69_04550

MGEO:

CFI10_14950

GBI:	PG2T_14045

RPF:	Rpic12D_2779 Rpic12D_4958

RPJ:	N234_12835

CNC:	CNE_2c21230(pht1)

CUH:	BJN34_09610 BJN34_28850

REU:	Reut_B4795 Reut_B5781

RME:

Rmet_5548

CBW:	RR42_s1578

CCUP:

BKK81_29815

CUP:	BKK80_26540

CUU:	BKK79_32425

COX:	E0W60_20460

CUK:	KB879_16005

BVI:	Bcep1808_4273

BCEN:

DM39_1999

BMJ:	BMULJ_03712(ophA2)

BMU:

Bmul_4805

BMK:

DM80_4020

BUB:	BW23_5728 BW23_5729

BUG:	BC1001_4039

BGO:

BM43_4032

BYI:	BYI23_B001650 BYI23_C002440 BYI23_D003170 BYI23_D008300

BUE:	BRPE67_BCDS04040 BRPE67_CCDS09780 BRPE67_DCDS04970 BRPE67_DCDS10220

BPLA:

bpln_1p1370

BURT:

BTHE68_67770

BXB:	DR64_1235 DR64_4702

BPH:	Bphy_3201 Bphy_4881 Bphy_4882 Bphy_6982

BGE:	BC1002_3595 BC1002_4693

BPX:	BUPH_00622 BUPH_02685

BPY:	Bphyt_7254

BUZ:	AYM40_12125 AYM40_32995 AYM40_33000

BCAI:

K788_0000443 K788_0005439

PSPW:

BJG93_04590

PARA:

BTO02_23060

PHS:	C2L64_49415

PTER:

C2L65_19050 C2L65_44680

PCJ:	CUJ87_29360

PEW:	KZJ38_26705

PPAI:

E1956_41115 E1956_41120

PPUL:

RO07_06175

PSPU:

NA29_21445

POX:	MB84_08085

PTX:	ABW99_00725

PFG:	AB870_18750

PNR:	AT302_05945 AT302_13310

PFIB:

PI93_011765 PI93_023915

PCOM:

NTU39_07750

PANN:

PanNE5_13500

PANX:

OYT13_00095

BUO:	BRPE64_DCDS03430 BRPE64_DCDS07110

CABK:

NK8_60880

BBH:	BN112_3649

BBM:	BN115_4450

BBX:	BBS798_4550

BPT:

Bpet0252

BHZ:	ACR54_04439(pht3)

BBRO:

BAU06_03620 BAU06_03750 BAU06_03780

BFZ:	BAU07_13845 BAU07_15835 BAU07_15865

BPDZ:

BBN53_00955

BOH:	AKI39_21300 AKI39_21330

BGM:	CAL15_17820

BOJ:	CBF45_02355

PUD:	G9Q38_00025 G9Q38_10670

POLL:

OEG81_03545

PHN:	PAEH1_01175

PIG:	EGT29_10005 EGT29_10580 EGT29_22455 EGT29_23240 EGT29_23705 EGT29_25710 EGT29_27735 EGT29_27745

KGY:	EHF36_02745

RHY:	RD110_25740

RHOB:

HTY51_11745 HTY51_17795

POL:	Bpro_1910 Bpro_2133 Bpro_5154

PNA:

Pnap_4627

POS:	DT070_19560 DT070_20425

POO:	F7R28_10150 F7R28_11010

ACID:

CBP33_05530

ACIP:

CBP36_05980 CBP36_20410

ACIN:

CBP34_05365

ACIS:

CBP35_12965 CBP35_20490

VEI:

Veis_2928

DEL:	DelCs14_1655 DelCs14_1661

DLA:	I6G47_32030 I6G47_32085 I6G47_32165 I6G47_32230 I6G47_33695

VPE:	Varpa_0165

VPD:	VAPA_1c01740 VAPA_2c09710

VBO:	CKY39_00895 CKY39_21970

VAM:	C4F17_18170 C4F17_30665

VAZ:	H7F35_12815

VAV:	IG196_31660

VAE:	RZE79_00920

CTT:	CtCNB1_1488

CTES:

O987_16325 O987_21865 O987_28200

CENP:

M9799_07590

RTA:

Rta_18000

OTK:	C6570_10585

LIM:	L103DPR2_00195(pht3_1) L103DPR2_00198(pht3_2) L103DPR2_02573(pht3_3) L103DPR2_02721(pht3_4)

LIH:	L63ED372_02473(pht3_1) L63ED372_03070(pht3_2)

LIMI:

LINBF2_03510

HYR:	BSY239_1474 BSY239_2699 BSY239_3345 BSY239_3465

HYB:	Q5W_00860 Q5W_13295 Q5W_24655

HYL:	LPB072_09690

HYC:	E5678_06755

HPSE:

HPF_14820(pht3)

HYN:	F9K07_00645 F9K07_00650 F9K07_00665 F9K07_06620 F9K07_11625 F9K07_11660 F9K07_11665 F9K07_11680 F9K07_13635 F9K07_22870 F9K07_27550 F9K07_30150 F9K07_30200 F9K07_30250 F9K07_30395 F9K07_30445

HTN:	KI616_03525 KI616_14275

HCZ:	G9Q37_05785

HYY:	JNX00_14915

DIM:	K2L43_12460

HGR:	DW355_02630

RGU:	A4W93_01640

MASW:

AM586_27410 AM586_27625 AM586_27690

XYK:	GT347_06320

RBU:	PG1C_09135

AZR:	CJ010_07345

THK:	CCZ27_15665

MESO:

BSQ44_11250

EMX:	FKV68_30120

STEG:

QA637_22935

ABAW:

D5400_03680 D5400_20255

AOL:

S58_21510

BRAD:

BF49_7248

BIC:	LMTR13_01465

BRK:	CWS35_04530 CWS35_19800

BOT:	CIT37_09645

BGQ:	X265_35300

BVZ:	BRAD3257_6131

BEL:	BE61_11040 BE61_64940 BE61_87020

BPAH:

QA639_41225

BVV:	BHK69_01760

BVES:

QO058_19460

BOI:	BLM15_16160

MLG:	CWB41_10140

RHZ:	RHPLAN_00120 RHPLAN_01370 RHPLAN_59330

DSAL:

K1X15_01480

HDI:	HDIA_4250(pht3)

MMED:

Mame_05096(pht3)

PSIN:

CAK95_02265 CAK95_05895

RBM:

TEF_13150

LABT:

FIU93_07195(pht1)

PHAE:

N1037_23055

CID:	P73_1053 P73_2968

CEH:	CEW89_13880

CBAK:

DA792_02400

CON:	TQ29_05745

TPRO:

Ga0080559_TMP5103

PABY:

Ga0080574_TMP2103

SUAM:

BOO69_02335

RID:	RIdsm_00905(pht3)

ROT:	FIV09_12260(pht5)

RMH:	LVO79_19250

MALU:

KU6B_35110

TGL:	HFZ77_01120

PALW:

PSAL_037190(pht3)

HDH:	G5B40_16605

LVR:	T8T21_19990

MANH:

LA6_006111(pht3_2)

NOR:	FA702_15515 FA702_19040

NDR:	HT578_08880

SPHP:

LH20_19475

SMAZ:

LH19_17785

RDI:	CMV14_06830 CMV14_06835

SPHI:

TS85_13270

SSY:	SLG_19120 SLG_34970

SPHR:

BSY17_4182

SHYD:

CJD35_19410 CJD35_19645

SPAG:

sphantq_04492(pht3_2)

SPCA:

GL174_20800 GL174_21630

PALG:

HFP57_16190

ACOB:

P0Y56_16120

SMIC:

SmB9_09290

POT:	E2E27_18200

RMT:	IAI58_16250

NAO:	Y958_23895

FER:	FNB15_09005

TLU:	R1T41_00620

THGO:

M9H61_18410

BFD:	NCTC4823_00630(kshA_1)

AIA:	AWH56_017995

PSAB:

PSAB_03650

PBJ:	VN24_18685

PNP:	IJ22_14360

EFF:	skT53_12430 skT53_33890

SPOS:

DV702_11120

MGAU:

MGALJ_59780

MRH:	MycrhN_3125

MGAD:

MGAD_32490

MGO:	AFA91_26505

MPAE:

K0O64_27755

MFRE:

EXE63_02915

NTP:	CRH09_08335

RRT:	4535765_03823(kshA_3)

RKO:	JWS14_40240

RHOW:

A4U64_03195

RHOJ:

JVH1_38360

RHOS:

QWW67_11425

RHOQ:

QWW98_11435

GOR:

KTR9_0709

GOQ:	ACH46_18515

SMAL:

SMALA_7807

SSOI:

I1A49_42875

SGM:	GCM10017557_02470

SPAD:

DVK44_00575

STUI:

GCM10017668_58100

SSPN:

LXH13_02865

SLUO:

PS467_02515

STRL:

HEP84_46895

SRPZ:

HEP85_06545

SALC:

C2138_02790

ACRY:

AC20117_22710

ASUF:

MNQ99_18620

PUE:	FV140_21245

ARZ:	AUT26_02365 AUT26_19495

GMY:

XH9_09030

NPS:	KRR39_10780

ACTQ:

OG417_26300

AACD:

LWP59_23325 LWP59_25695

ARHD:

VSH64_09950 VSH64_16450

ACAB:

QRX50_19585 QRX50_37835 QRX50_49170

AMYD:

K1T34_01090 K1T34_02405

PDX:	Psed_1414 Psed_3698 Psed_3921

PSDS:

K1T35_28420

PMAD:

BAY61_17900

SAQ:

Sare_0574

PFLA:

Pflav_042940 Pflav_042960

PSUU:

Psuf_003970 Psuf_021450 Psuf_075920

DACT:

KZZ52_33355

LEPV:

J4558_17010(rpsC)

VAB:

WPS_26940

» show all

Reference

1 [PMID:3805038]

Authors

Batie CJ, LaHaie E, Ballou DP.

Title

Purification and characterization of phthalate oxygenase and phthalate oxygenase reductase from Pseudomonas cepacia.

Journal

J Biol Chem 262:1510-8 (1987)

Other DBs

ExplorEnz - The Enzyme Database:

1.14.12.7

IUBMB Enzyme Nomenclature:

1.14.12.7

ExPASy - ENZYME nomenclature database:

1.14.12.7

UM-BBD (Biocatalysis/Biodegradation Database):

1.14.12.7

BRENDA, the Enzyme Database:

1.14.12.7

CAS:	63626-44-8

LinkDB

DBGET integrated database retrieval system