KEGG   ENZYME: 1.14.12.9
Entry
EC 1.14.12.9                Enzyme                                 
Name
4-chlorophenylacetate 3,4-dioxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
Sysname
4-chlorophenylacetate,NADH:oxygen oxidoreductase (3,4-hydroxylating, dechlorinating)
Reaction(IUBMB)
4-chlorophenylacetate + NADH + H+ + O2 = 3,4-dihydroxyphenylacetate + chloride + NAD+ [RN:R03306]
Reaction(KEGG)
R03306
Substrate
4-chlorophenylacetate [CPD:C03077];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
3,4-dihydroxyphenylacetate [CPD:C01161];
chloride [CPD:C00698];
NAD+ [CPD:C00003]
Comment
A system, containing a reductase and an iron-sulfur oxygenase, and no independent ferredoxin. Requires Fe2+. Also acts on 4-bromophenyl acetate.
History
EC 1.14.12.9 created 1989 as EC 1.13.99.4, transferred 1992 to EC 1.14.12.9
Pathway
ec00350  Tyrosine metabolism
ec01120  Microbial metabolism in diverse environments
Reference
1  [PMID:3745216]
  Authors
Markus A, Krekel D, Lingens F.
  Title
Purification and some properties of component A of the 4-chlorophenylacetate 3,4-dioxygenase from Pseudomonas species strain CBS.
  Journal
J Biol Chem 261:12883-8 (1986)
Other DBs
ExplorEnz - The Enzyme Database: 1.14.12.9
IUBMB Enzyme Nomenclature: 1.14.12.9
ExPASy - ENZYME nomenclature database: 1.14.12.9
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.12.9
BRENDA, the Enzyme Database: 1.14.12.9
CAS: 105006-00-6
LinkDB

KEGG   REACTION: R03306
Entry
R03306                      Reaction                               
Name
4-chlorophenylacetate,NADH:oxygen oxidoreductase (3,4-hydroxylating, dechlorinating)
Definition
4-Chlorophenylacetate + Oxygen + NADH + H+ <=> 3,4-Dihydroxyphenylacetate + Hydrochloric acid + NAD+
Equation
C03077 + C00007 + C00004 + C00080 <=> C01161 + C01327 + C00003
Reaction class
RC00001  C00003_C00004
RC00901  C01161_C03077
Enzyme
1.14.12.9
Pathway
rn00350  Tyrosine metabolism
rn01120  Microbial metabolism in diverse environments
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.12  With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
    1.14.12.9
     R03306  4-Chlorophenylacetate + Oxygen + NADH + H+ <=> 3,4-Dihydroxyphenylacetate + Hydrochloric acid + NAD+
Other DBs
RHEA: 14692
LinkDB

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