KEGG   ENZYME: 1.14.13.111
Entry
EC 1.14.13.111              Enzyme                                 
Name
methanesulfonate monooxygenase (NADH);
mesylate monooxygenase;
mesylate,reduced-FMN:oxygen oxidoreductase;
MsmABC;
methanesulfonic acid monooxygenase;
MSA monooxygenase;
MSAMO
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
methanesulfonate,NADH:oxygen oxidoreductase
Reaction(IUBMB)
methanesulfonate + NADH + H+ + O2 = formaldehyde + NAD+ + sulfite + H2O [RN:R09513]
Reaction(KEGG)
R09513
Substrate
methanesulfonate [CPD:C11145];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
formaldehyde [CPD:C00067];
NAD+ [CPD:C00003];
sulfite [CPD:C00094];
H2O [CPD:C00001]
Comment
A flavoprotein. Methanesulfonate is the simplest of the sulfonates and is a substrate for the growth of certain methylotrophic microorganisms. Compared with EC 1.14.14.5, alkanesulfonate monooxygenase, this enzyme has a restricted substrate range that includes only the short-chain aliphatic sulfonates (methanesulfonate to butanesulfonate) and excludes all larger molecules, such as arylsulfonates [1]. The enzyme from the bacterium Methylosulfonomonas methylovora is a multicomponent system comprising a hydroxylase, a reductase (MsmD) and a ferredoxin (MsmC). The hydroxylase has both large (MsmA) and small (MsmB) subunits, with each large subunit containing a Rieske-type [2Fe-2S] cluster. cf. EC 1.14.14.34, methanesulfonate monooxygenase (FMNH2).
History
EC 1.14.13.111 created 2009 as EC 1.14.14.6, transferred 2010 to EC 1.14.13.111, modified 2016
Pathway
ec00920  Sulfur metabolism
ec01100  Metabolic pathways
Orthology
K16968  methanesulfonate monooxygenase subunit alpha
K16969  methanesulfonate monooxygenase subunit beta
Genes
PQIKH389_17550 KH389_17555
CUHBJN34_09325 BJN34_09330 BJN34_22320
BCTGEM_5811 GEM_5812
BUEBRPE67_FCDS01800
PPKU875_05270 U875_05275
PPNODA70_22340 DA70_22345
PRBX636_04320 X636_04325
BPABPP0512 BPP0513
BBHBN112_2903 BN112_2904
BBRBB0517 BB0518
BBMBN115_0496 BN115_0497
BBXBBS798_0510 BBS798_0511
BTRMSAMEA390648701717(antA)
PUTPT7_2465 PT7_2466
PUDG9Q38_01125 G9Q38_01130
MPTMpe_A0919(msmS2) Mpe_A0920(msmB)
METPC1M51_13795
SPLBSFPGR_26190 SFPGR_26200
MNOMnod_2707 Mnod_2708 Mnod_8364 Mnod_8365
FILBN1229_v1_3548(msmB) BN1229_v1_3549(msmA)
FIYBN1229_v1_2374(msmA) BN1229_v1_2375(msmB)
APBSAR116_2102 SAR116_2103 SAR116_2109
 » show all
Reference
1  [PMID:10094704]
  Authors
de Marco P, Moradas-Ferreira P, Higgins TP, McDonald I, Kenna EM, Murrell JC
  Title
Molecular analysis of a novel methanesulfonic acid monooxygenase from the methylotroph Methylosulfonomonas methylovora.
  Journal
J Bacteriol 181:2244-51 (1999)
DOI:10.1128/JB.181.7.2244-2251.1999
  Sequence
Reference
2  [PMID:8932698]
  Authors
Higgins TP, Davey M, Trickett J, Kelly DP, Murrell JC
  Title
Metabolism of methanesulfonic acid involves a multicomponent monooxygenase enzyme.
  Journal
Microbiology 142 ( Pt 2):251-60 (1996)
DOI:10.1099/13500872-142-2-251
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.111
IUBMB Enzyme Nomenclature: 1.14.13.111
ExPASy - ENZYME nomenclature database: 1.14.13.111
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.13.111
BRENDA, the Enzyme Database: 1.14.13.111
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