Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
erythromycin-D,NADPH:oxygen oxidoreductase (12-hydroxylating)
erythromycin D + NADPH + H+ + O2 = erythromycin C + NADP+ + H2O [RN:
The enzyme is responsible for the C-12 hydroxylation of the macrolactone ring, one of the last steps in erythromycin biosynthesis. It shows 1200-1900-fold preference for erythromycin D over the alternative substrate erythromycin B .
EC 22.214.171.124 created 2012
ec00522 Biosynthesis of 12-, 14- and 16-membered macrolides
ec01110 Biosynthesis of secondary metabolites
K14370 erythromycin 12 hydroxylase
Lambalot RH, Cane DE, Aparicio JJ, Katz L
Overproduction and characterization of the erythromycin C-12 hydroxylase, EryK.
Savino C, Montemiglio LC, Sciara G, Miele AE, Kendrew SG, Jemth P, Gianni S, Vallone B
Investigating the structural plasticity of a cytochrome P450: three-dimensional structures of P450 EryK and binding to its physiological substrate.
Montemiglio LC, Gianni S, Vallone B, Savino C
Azole drugs trap cytochrome P450 EryK in alternative conformational states.
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