KEGG ENZYME: 1.14.13.212

ENZYME: 1.14.13.212

Entry

EC 1.14.13.212              Enzyme

Name

1,3,7-trimethyluric acid 5-monooxygenase;
tmuM (gene name)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

1,3,7-trimethylurate,NADH:oxygen oxidoreductase (1,3,7-trimethyl-5-hydroxyisourate-forming)

Reaction(IUBMB)

1,3,7-trimethylurate + NADH + H+ + O2 = 1,3,7-trimethyl-5-hydroxyisourate + NAD+ + H2O [RN:R11153]

Reaction(KEGG)

R11153

Substrate

1,3,7-trimethylurate [CPD:C16361];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]

Product

1,3,7-trimethyl-5-hydroxyisourate [CPD:C21144];
NAD+ [CPD:C00003];
H2O [CPD:C00001]

Comment

The enzyme, characterized from the bacterium Pseudomonas sp. CBB1, is part of the bacterial C-8 oxidation-based caffeine degradation pathway. The product decomposes spontaneously to a racemic mixture of 3,6,8-trimethylallantoin. The enzyme shows no acitivity with urate. cf. EC 1.14.13.113, FAD-dependent urate hydroxylase.

History

EC 1.14.13.212 created 2016

Pathway

ec00232

Caffeine metabolism

ec01120

Microbial metabolism in diverse environments

Orthology

K00492

1,3,7-trimethyluric acid 5-monooxygenase

Reference

1 [PMID:22609920]

Authors

Mohanty SK, Yu CL, Das S, Louie TM, Gakhar L, Subramanian M

Title

Delineation of the caffeine C-8 oxidation pathway in Pseudomonas sp. strain CBB1 via characterization of a new trimethyluric acid monooxygenase and genes involved in trimethyluric acid metabolism.

Journal

J Bacteriol 194:3872-82 (2012)
DOI:10.1128/JB.00597-12

Sequence

[ag:AFO10115]

Reference

2 [PMID:25678373]

Authors

Summers RM, Mohanty SK, Gopishetty S, Subramanian M

Title

Genetic characterization of caffeine degradation by bacteria and its potential applications.

Journal

Microb Biotechnol 8:369-78 (2015)
DOI:10.1111/1751-7915.12262