Entry
Name
magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase;
Mg-protoporphyrin IX monomethyl ester (oxidative) cyclase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
magnesium-protoporphyrin-IX 13-monomethyl ester,NADPH:oxygen oxidoreductase (hydroxylating)
Reaction(IUBMB)
magnesium-protoporphyrin IX 13-monomethyl ester + 3 NADPH + 3 H+ + 3 O2 = 3,8-divinyl protochlorophyllide a + 3 NADP+ + 5 H2O (overall reaction) [RN:
R10068 ];
(1a) magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + H+ + O2 = 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + H2O [RN:
R06265 ];
(1b) 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + H+ + O2 = 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + 2 H2O [RN:
R06266 ];
(1c) 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + H+ + O2 = 3,8-divinyl protochlorophyllide a + NADP+ + 2 H2O [RN:
R06267 ]
Reaction(KEGG)
Substrate
magnesium-protoporphyrin IX 13-monomethyl ester [CPD:
C04536 ];
NADPH [CPD:
C00005 ];
H+ [CPD:
C00080 ];
O2 [CPD:
C00007 ];
131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester;
131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester
Product
3,8-divinyl protochlorophyllide a [CPD:
C11831 ];
NADP+ [CPD:
C00006 ];
H2O [CPD:
C00001 ];
131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester;
131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester
Comment
Requires iron(II) for activity. The enzyme participates in the biosynthesis of chlorophyllide a in aerobic organisms. The same transformation is achieved in anaerobic organisms by EC
1.21.98.3 , anaerobic magnesium-protoporphyrin IX monomethyl ester cyclase. Some facultative phototrophic bacteria, such as Rubrivivax gelatinosus, possess both enzymes.
History
EC 1.14.13.81 created 2003, modified 2017
Pathway
ec01110 Biosynthesis of secondary metabolites
Orthology
K04035 magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase
Genes
CSAT : 104712356 104781590 104791984
BNA : 106368040 106405693 106445907 111208747
HSYR : 120135502 120198142
LANG : 109337037 109357310 109358322
QSA : O6P43_006283 O6P43_009985
CMAX : 111466024 111478142
CMOS : 111432321 111453398
CPEP : 111781371 111803339
PALZ : 118033159 118052967
SIND : 105155783 105162815
SSPL : 121749452 121752943 121795794 121799459
SHIS : 125189047 125194239
AEW : 130751723 130772566 130772754
PSOM : 113309376 113322506
TAES : 100037649 123061131 123078196
MFLO : 136449835 136449853 136455943
NCOL : 116259868 116264152
SMO : SELMODRAFT_267431 SELMODRAFT_89989
CRE : CHLRE_07g346050v5 CHLRE_12g510050v5
VCN : VOLCADRAFT_80084 VOLCADRAFT_81575
CVR : CHLNCDRAFT_144710 CHLNCDRAFT_48731
MMES : MMSR116_22300(acsF)
RLI : RLO149_p940810(acsF)
SYN : sll1214 sll1874(AT103)
SYZ : MYO_116600(at103) MYO_150
SYY : SYNGTS_0005(sll1214) SYNGTS_1645(chlAII)
SYT : SYNGTI_0005(sll1214) SYNGTI_1645(chlAII)
SYS : SYNPCCN_0005(sll1214) SYNPCCN_1644(chlAII)
SYQ : SYNPCCP_0005(sll1214) SYNPCCP_1644(chlAII)
SYJ : D082_08250(AT103) D082_11950(ycf59)
SYO : C7I86_00025(acsF) C7I86_08535(acsF)
SYNH : HTZ78_06370(acsF) HTZ78_15560(acsF)
SYR : SynRCC307_1476(acsF)
SYX : SynWH7803_1102(acsF)
SYNP : Syn7502_00420 Syn7502_03308
SYNW : SynWH8103_01332(acsF)
SYNC : CB0101_00325(acsF) CB0101_01670(acsF)
SYNY : BM449_01900 BM449_05810
DSL : Dacsa_1976 Dacsa_3627
CGC : Cyagr_0706 Cyagr_1039
CYI : CBM981_0231(acsF) CBM981_1951(acsF)
PMC : P9515_09201(pnilL34)
PMJ : P9211_09191(pniIL34)
CYN : Cyan7425_4340 Cyan7425_4781
TVN : NIES2134_111220(ycf59) NIES2134_116400
TEL : tlr1426(ycf59) tlr1722
THN : NK55_02575(acsF1) NK55_05260(acsF2)
THEC : FFX45_07110(acsF) FFX45_08845(acsF)
THEY : JW907_04125(acsF) JW907_11175(acsF)
TSQ : D3A95_10075(acsF) D3A95_13065(acsF)
AMR : AM1_0465(acsF) AM1_2295(acsF)
TSZ : OOK60_01680(acsF) OOK60_15380(acsF)
LBO : LBWT_34030 LBWT_42950
LEPV : J4558_20220(acsF) J4558_20670(acsF)
KOV : K9N68_02375(acsF) K9N68_36290(acsF)
LSC : KIK02_06920(acsF) KIK02_21825(acsF)
LSK : J5X98_15830(acsF) J5X98_19820(acsF)
PSER : ABRG53_0108(acsF) ABRG53_3949(acsF)
PGX : OA858_12930(acsF) OA858_16800(acsF)
CHON : NIES4102_11510 NIES4102_38690
GEN : GM3709_1534 GM3709_1815
GEE : GM3708_585 GM3708_779
CAN : Cyan10605_1422 Cyan10605_2707
CSN : Cyast_0064 Cyast_2869
CYL : AA637_00120(acsF) AA637_14735(acsF-2)
SYP : SYNPCC7002_A0707(acsF) SYNPCC7002_A1992(acsF)
SYV : AWQ23_03685 AWQ23_09990
SYL : AWQ21_03545 AWQ21_08625
LEP : Lepto7376_1355 Lepto7376_4048
SYNN : NIES970_02170(acsF_1) NIES970_03320(acsF_2)
PIP : FEK30_07640(acsF) FEK30_14270(acsF)
MVZ : myaer102_13940 myaer102_20250
HAO : PCC7418_1706 PCC7418_3522
ENN : FRE64_04905(acsF) FRE64_09065(acsF)
CYU : UCYN_08810 UCYN_11800
CWA : CwatDRAFT_3460 CwatDRAFT_6122
CYP : PCC8801_2261 PCC8801_3705
CYH : Cyan8802_2312 Cyan8802_3758
CYC : PCC7424_2605 PCC7424_3134
CYJ : Cyan7822_4263 Cyan7822_5395
PPSU : NO713_00620(acsF3) NO713_03023(acsF2)
PRUN : PCC7821_02993(acsF3) PCC7821_03584(acsF2)
OXY : HCG48_01810(acsF) HCG48_10825(acsF)
OAC : Oscil6304_5538 Oscil6304_5882
ONI : Osc7112_1426 Osc7112_2921
PHOR : JWS08_05890(acsF) JWS08_18920(acsF)
PYH : NEA10_06635(acsF) NEA10_13910(acsF)
MIC : Mic7113_1589 Mic7113_5321
GEI : GEI7407_1972 GEI7407_2883
ANA : all1880 alr1358 alr3300
NPU : Npun_F1653 Npun_F6446
NOS : Nos7107_1361 Nos7107_5431
NOP : Nos7524_0896 Nos7524_3425 Nos7524_3949
NON : NOS3756_16090 NOS3756_44890
NFL : COO91_01779 COO91_03121
NOE : CLI64_10670(acsF) CLI64_21635(acsF) CLI64_28005(acsF)
NED : HUN01_03560(acsF) HUN01_10900(acsF)
NPZ : ACX27_17390 ACX27_21730 ACX27_30385
NLK : NIES25_01850 NIES25_38480
NCW : NIES2107_19620 NIES2107_65760 NIES2107_67350
NCF : ANSO36C_58420(acsF1) ANSO36C_61400(acsF2)
ANB : ANA_C10475(acsF) ANA_C11197(acsF)
ACY : Anacy_0238 Anacy_3447 Anacy_5348
AWA : AA650_00580 AA650_05545
ANN : EH233_00590(acsF) EH233_12300(acsF)
CSG : Cylst_1264 Cylst_3224
RSIN : B6N60_00143 B6N60_03087 B6N60_05088
HBQ : QI031_11460(acsF) QI031_28550(acsF)
CALT : Cal6303_0508 Cal6303_0540 Cal6303_3632
CALH : IJ00_00755 IJ00_01475
CALN : NIES2098_22160 NIES2098_52250
CALS : NIES3974_23050 NIES3974_47770
RIV : Riv7116_2518 Riv7116_3605
MDP : NIES3275_21140 NIES3275_39540 NIES3275_49990
FIS : FIS3754_05810 FIS3754_12410
NSP : BMF81_00211(acsF) BMF81_04265(acsF_2)
NSPH : BDGGKGIB_01525 BDGGKGIB_03679 BDGGKGIB_04480
DOU : BMF77_02276 BMF77_02422
DFS : HGD76_05435(acsF) HGD76_06245(acsF)
DHT : NG743_14545(acsF) NG743_21430(acsF)
DCM : NIES806_40090 NIES806_44210
DOT : EZY12_09305(acsF) EZY12_11375(acsF)
DOZ : HEP80_02640(acsF) HEP80_24665(acsF)
DOC : HEQ26_04655(acsF) HEQ26_13900(acsF)
AFLO : HEQ12_04350(acsF) HEQ12_08955(acsF)
STOQ : K2F26_04720(acsF) K2F26_22955(acsF)
SKV : NIES73_17080 NIES73_46550
AEE : IM676_02755(acsF) IM676_09755(acsF) IM676_13220(acsF) IM676_15170(acsF)
OCF : WJM97_05805(acsF) WJM97_08810(acsF)
TOQ : HCG51_06915(acsF) HCG51_14440(acsF) HCG51_32660(acsF)
TTQ : NIES37_40840 NIES37_61450 NIES37_66480
ALAX : NIES50_20090 NIES50_25120 NIES50_45750
SCYT : SAMD_00710 SAMD_74470
SCYS : NIES4073_16100 NIES4073_24700
BSEN : DP114_24750(acsF) DP114_25630(acsF)
BOX : DP113_24665(acsF) DP113_25540(acsF)
NCN : BZZ01_00630 BZZ01_14365
PLP : Ple7327_2340 Ple7327_3805
STAN : STA3757_30850 STA3757_37100
PSPC : Strain318_000130(acsF)
» show all
Taxonomy
Reference
Authors
Walker CJ, Mansfield KE, Rezzano IN, Hanamoto CM, Smith KM, Castelfranco PA
Title
The magnesium-protoporphyrin IX (oxidative) cyclase system. Studies on the mechanism and specificity of the reaction sequence.
Journal
Biochem J 255:685-92 (1988)
Reference
Authors
Bollivar DW, Beale SI.
Title
The Chlorophyll Biosynthetic Enzyme Mg-Protoporphyrin IX Monomethyl Ester (Oxidative) Cyclase (Characterization and Partial Purification from Chlamydomonas reinhardtii and Synechocystis sp. PCC 6803).
Journal
Reference
Authors
Pinta V, Picaud M, Reiss-Husson F, Astier C
Title
Rubrivivax gelatinosus acsF (previously orf358) codes for a conserved, putative binuclear-iron-cluster-containing protein involved in aerobic oxidative cyclization of Mg-protoporphyrin IX monomethylester.
Journal
Reference
Authors
Tottey S, Block MA, Allen M, Westergren T, Albrieux C, Scheller HV, Merchant S, Jensen PE
Title
Arabidopsis CHL27, located in both envelope and thylakoid membranes, is required for the synthesis of protochlorophyllide.
Journal
Sequence
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