KEGG   ENZYME: 1.14.14.111
Entry
EC 1.14.14.111              Enzyme                                 
Name
9beta-pimara-7,15-diene oxidase;
CYP99A3;
9beta-pimara-7,15-diene monooxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
9beta-pimara-7,15-diene,[reduced NADPH---hemoprotein reductase]:oxygen 19-oxidoreductase
Reaction(IUBMB)
9beta-pimara-7,15-diene + 3 O2 + 3 [reduced NADPH---hemoprotein reductase] = 9beta-pimara-7,15-dien-19-oate + 3 [oxidized NADPH---hemoprotein reductase] + 4 H2O (overall reaction) [RN:R09865];
(1a) 9beta-pimara-7,15-diene + O2 + [reduced NADPH---hemoprotein reductase] = 9beta-pimara-7,15-dien-19-ol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R09862];
(1b) 9beta-pimara-7,15-dien-19-ol + O2 + [reduced NADPH---hemoprotein reductase] = 9beta-pimara-7,15-dien-19-al + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN:R09863];
(1c) 9beta-pimara-7,15-dien-19-al + O2 + [reduced NADPH---hemoprotein reductase] = 9beta-pimara-7,15-dien-19-oate + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R09864]
Reaction(KEGG)
R09862 R09863 R09864 R09865;
(other) R09921
Substrate
9beta-pimara-7,15-diene [CPD:C18225];
O2 [CPD:C00007];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
9beta-pimara-7,15-dien-19-ol [CPD:C20147];
9beta-pimara-7,15-dien-19-al [CPD:C20148]
Product
9beta-pimara-7,15-dien-19-oate [CPD:C20149];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001];
9beta-pimara-7,15-dien-19-ol [CPD:C20147];
9beta-pimara-7,15-dien-19-al [CPD:C20148]
Comment
A cytochrome P-450 (heme-thiolate) protein. The enzyme from rice (Oryza sativa) is involved in the biosynthesis of the phytoalexin momilactone. It also acts similarly on 9beta-stemod-13(17)-ene.
History
EC 1.14.14.111 created 2012 as EC 1.14.13.144, transferred 2018 to EC 1.14.14.111
Pathway
ec00904  Diterpenoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K16085  9beta-pimara-7,15-diene oxidase
Genes
OSA: 4335091(CYP99A3) 4335096(CYP99A2)
OBR: 102705642 102721444
OGL: 127769969 127770008
BDI: 100836875
ATS: 109731407 109734305 109743692 109750535 109773965 109773967 109773970 109774538 109776121 109780586 109780589
TDC: 119271910 119272669 119278132 119281969 119351866 119356226 119358650 119368198
TAES: 123041873 123041878 123043471 123046476 123048254 123048256 123048257 123048258 123049849 123049853 123054377 123055487 123057860 123063080 123064868 123071979 123074029 123075233 123100267 123126305 123169592 123184294 123184298 123184300 123184301 123184302 123185921 123185949 123190208
TUA: 125528121 125533911 125533928 125540541 125540674 125547908 125549032
LPER: 127292356 127318419 127336229 127336234 127337247 127338821 127345412
LRD: 124651229 124667729 124667960 124668042 124672078 124672080 124676243 124677570 124679047 124706022 124706208
SBI: 8069842 8080269 8081807 8085991
ZMA: 100273010 103641402 103652787
MFLO: 136472920 136482447 136483129 136485655 136494638 136495602 136545587
SITA: 101767767 101772065 101772739 101776139 101776959
SVS: 117841995 117842511 117859811 117860031 117860109 117865140
PVIR: 120651251 120660277 120674828 120690916
PHAI: 112879668 112896256
 » show all
Reference
1  [PMID:21175892]
  Authors
Wang Q, Hillwig ML, Peters RJ
  Title
CYP99A3: functional identification of a diterpene oxidase from the momilactone biosynthetic gene cluster in rice.
  Journal
Plant J 65:87-95 (2011)
DOI:10.1111/j.1365-313X.2010.04408.x
  Sequence
[osa:4335091]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.111
IUBMB Enzyme Nomenclature: 1.14.14.111
ExPASy - ENZYME nomenclature database: 1.14.14.111
BRENDA, the Enzyme Database: 1.14.14.111
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