KEGG   ENZYME: 1.14.14.125
Entry
EC 1.14.14.125              Enzyme                                 
Name
monacolin L hydroxylase;
LovA (ambiguous)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
monacolin L acid,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (8-hydroxylating)
Reaction(IUBMB)
monacolin L acid + O2 + [reduced NADPH---hemoprotein reductase] = monacolin J acid + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R10794]
Reaction(KEGG)
R10794
Substrate
monacolin L acid [CPD:C20853];
O2 [CPD:C00007];
[reduced NADPH---hemoprotein reductase] [CPD:C03024]
Product
monacolin J acid [CPD:C20854];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein. The enzyme from fungi also catalyses the reaction of EC 1.14.14.124, dihydromonacolin L hydroxylase.
History
EC 1.14.14.125 created 2014 as EC 1.14.13.198, transferred 2018 to EC 1.14.14.125
Pathway
ec00997  Biosynthesis of various other secondary metabolites
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K20980  dihydromonacolin L/monacolin L hydroxylase
Reference
1  [PMID:21495633]
  Authors
Barriuso J, Nguyen DT, Li JW, Roberts JN, MacNevin G, Chaytor JL, Marcus SL, Vederas JC, Ro DK
  Title
Double oxidation of the cyclic nonaketide dihydromonacolin L to monacolin J by a single cytochrome P450 monooxygenase, LovA.
  Journal
J Am Chem Soc 133:8078-81 (2011)
DOI:10.1021/ja201138v
  Sequence
[ag:AAD34552]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.125
IUBMB Enzyme Nomenclature: 1.14.14.125
ExPASy - ENZYME nomenclature database: 1.14.14.125
BRENDA, the Enzyme Database: 1.14.14.125
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