KEGG   ENZYME: 1.14.14.147
Entry
EC 1.14.14.147              Enzyme                                 
Name
22alpha-hydroxysteroid 23-monooxygenase;
cytochrome P450 90C1;
CYP90D1;
CYP90C1;
3-epi-6-deoxocathasterone,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (C-23-hydroxylating);
3-epi-6-deoxocathasterone 23-monooxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
22alpha-hydroxysteroid,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (C-23-hydroxylating)
Reaction(IUBMB)
(1) 3-epi-6-deoxocathasterone + [reduced NADPH---hemoprotein reductase] + O2 = 6-deoxotyphasterol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R07792];
(2) (22S,24R)-22-hydroxy-5alpha-ergostan-3-one + [reduced NADPH---hemoprotein reductase] + O2 = 3-dehydro-6-deoxoteasterone + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R07791]
Reaction(KEGG)
R07791 R07792;
(other) R07786 R07787
Substrate
3-epi-6-deoxocathasterone [CPD:C16251];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007];
(22S,24R)-22-hydroxy-5alpha-ergostan-3-one [CPD:C15797]
Product
6-deoxotyphasterol [CPD:C15801];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001];
3-dehydro-6-deoxoteasterone [CPD:C15800]
Comment
A cytochrome P-450 (heme-thiolate) protein involved in brassinosteroid biosynthesis in plants. The enzyme has a relaxed substrate specificity, and C-23 hydroxylation can occur at different stages in the pathway. In Arabidopsis thaliana two isozymes, encoded by the CYP90C1 and CYP90D1 genes, have redundant activities.
History
EC 1.14.14.147 created 2010 as EC 1.14.13.112, transferred 2018 to EC 1.14.14.147, modified 2022
Pathway
ec00905  Brassinosteroid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K12637  3-epi-6-deoxocathasterone 23-monooxygenase
K12638  3-epi-6-deoxocathasterone 23-monooxygenase
Genes
ATH: AT3G13730(CYP90D1) AT4G36380(ROT3)
ALY: 9298835 9303091 9321051
CRB: 17877976 17893533
CSAT: 104716496(ROT3) 104721136 104733119 104745632 104765165
EUS: EUTSA_v10020591mg EUTSA_v10024901mg
BRP: 103842602 103862467 103868633 103870101
BNA: 106350972 106351089 106369862 106409581 106426522 106440615 106451959 106452300
BOE: 106302041 106307279 106327160 106344076
RSZ: 108805921 108839589 108849049 108857636
THJ: 104807476 104810577
CPAP: 110818395 110822476
CIT: 102610008 102623590
CIC: CICLE_v10025448mg CICLE_v10025479mg
PVY: 116106176 116118949
MINC: 123198912 123205796 123219507 123219819
TCC: 18596114 18610843
GRA: 105769910 105787502 105803759
GHI: 107906110 107913036 107936555 107943285 121209822
GAB: 108451933 108458395 108472765
DZI: 111274623 111280433 111293281 111301082 111316248
EGR: 104422732 104449963
GMX: 100782687 100797868 100799030 100812489
GSJ: 114375691 114381782 114417944 114420172 114422309
PVU: PHAVU_002G167400g PHAVU_003G258400g PHAVU_010G139600g
VRA: 106768728 106773857 106777471
VAR: 108323332 108335338 108336674
VUN: 114166334 114174236 114179716
CCAJ: 109801110 109809062 109814118
APRC: 113851021 113856000 113858772 113870295
MTR: 11405666 11406632 25500440
CAM: 101488381 101505609 101509993
PSAT: 127074637 127076400 127076401 127120195
LJA: Lj2g3v1728770.1(Lj2g3v1728770.1) Lj2g3v1728770.2(Lj2g3v1728770.2) Lj3g3v2693130.1(Lj3g3v2693130.1) Lj3g3v2693130.2(Lj3g3v2693130.2)
ADU: 107461434 107464245 107488015
AIP: 107612705 107618558 107642922
AHF: 112706501 112710103 112749491 112772650 112777343 112800770
LANG: 109348710 109358278
FVE: 101297721 101302700
RCN: 112187971 112188662
PPER: 18771246 18784338
PMUM: 103327717 103340160
PAVI: 110767804 110773923
PDUL: 117622280 117636128
MDM: 103400874 103431557 103453669
PXB: 103934102 103957186
ZJU: 107403831 107424559
MNT: 112094164 21388039
CSV: 101203911 101212581 101217830
CMO: 103485087 103490016 103500734
BHJ: 120073232 120086051 120091319
MCHA: 111005423 111016959
CMAX: 111479936 111496682
CMOS: 111458064 111463512
CPEP: 111782027 111790258
RCU: 8266971 8279118
JCU: 105631477 105639077
HBR: 110639585 110648648 110668522
MESC: 110608590 110626427
POP: 18096744 7465155 7495513
PEU: 105114341 105118255 105118442 105126648
PALZ: 118030995 118040884 118063091
JRE: 108991288 108992064 109007914
CILL: 122281481 122300963 122317271
QSU: 111995943 112000874 112003250
QLO: 115962687 115989007 115994450
TWL: 120013070 120015527
VVI: 100259060 100259945
VRI: 117912322 117921515
SLY: 101245605 101256170
SPEN: 107009038 107014078
SOT: 102582597 102598106
SSTN: 125849542 125859968
CANN: 107860019 107861744
NTA: 107771928 107795325 107795780 107816861
NSY: 104213362 104213597
NTO: 104118978 104121493
NAU: 109210485 109231818
INI: 109177767 109190236
ITR: 116000340 116023130
SIND: 105157742 105165748
OEU: 111367697
EGT: 105965330 105975773
SSPL: 121767418 121768407 121771701 121771730
APAN: 127244242 127262812
HAN: 110886538 110894209 110915370
ECAD: 122579553 122596266
LSV: 111897310 111910313 122194394 128125930 128132646 128132647 128132648 128132649 128132650
CCAV: 112513182 112517254
DCR: 108219828 108220552
CSIN: 114270454 114279029 114309759
BVG: 104895094 104900369
SOE: 110785866 110798894
CQI: 110682556 110684296 110685861 110719834
NNU: 104586612 104595594 104610557
MING: 122074479 122078744 122089610
TSS: 122653626 122661980 122667105
PSOM: 113288714 113288788 113347873 113349511
PDA: 103712873 103718648 103723970
EGU: 105040406 105042258 105047955 105059996
MUS: 103969391 103975346 103991264 103995745 103999307
ZOF: 121976062 122044032
DCT: 110094797 110107619
PEQ: 110020222 110022604
AOF: 109828140 109835455
NCOL: 116256449 116256903
ATR: 18429333 18448141
SMO: SELMODRAFT_151754(ROT3B-1) SELMODRAFT_157387(CYP90E3) SELMODRAFT_182839(ROT3A-2)
 » show all
Reference
1  [PMID:15703058]
  Authors
Kim GT, Fujioka S, Kozuka T, Tax FE, Takatsuto S, Yoshida S, Tsukaya H.
  Title
CYP90C1 and CYP90D1 are involved in different steps in the brassinosteroid biosynthesis pathway in Arabidopsis thaliana.
  Journal
Plant J 41:710-21 (2005)
DOI:10.1111/j.1365-313X.2004.02330.x
Reference
2  [PMID:17138693]
  Authors
Ohnishi T, Szatmari AM, Watanabe B, Fujita S, Bancos S, Koncz C, Lafos M, Shibata K, Yokota T, Sakata K, Szekeres M, Mizutani M
  Title
C-23 hydroxylation by Arabidopsis CYP90C1 and CYP90D1 reveals a novel shortcut in brassinosteroid biosynthesis.
  Journal
Plant Cell 18:3275-88 (2006)
DOI:10.1105/tpc.106.045443
  Sequence
[ath:AT4G36380 AT3G13730]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.147
IUBMB Enzyme Nomenclature: 1.14.14.147
ExPASy - ENZYME nomenclature database: 1.14.14.147
BRENDA, the Enzyme Database: 1.14.14.147
LinkDB

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